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M D Bishop

Publications and source records attributed to M D Bishop.

At least 37 records · Page 2Linked to original sources
PubMed1996

Development of a bovine X chromosome linkage group and painting probes to assess cattle, sheep, and goat X chromosome segment homologies.

The X chromosome linkage group is conserved in placental mammals. However, X chromosome morphological differences, due to internal chromosome rearrangements, exist among mammalian species. We have developed bovine chromosome painting probes for Xp and Xq to assess segment homologies between the submetacentric bovine X chromosome and the acrocentric sheep and goat X chromosomes. These painting probes and their corresponding DNA libraries were developed by chromosome micromanipulation, DNA micropurification, microcloning, and PCR amplification. The bovine Xp painting probe identified an interstitially located homologous segment in the sheep and goat Xq region, most probably resulting from chromosome inversion. Ten type II (microsatellite) markers obtained from the bovine Xq library and five other X chromosome assigned, but unlinked, markers were used to generate a linkage map for Xq spanning 89.4 centimorgans. The chromosome painting probes and molecular markers generated in this study would be useful for comparative mapping and tracing of internal X chromosome rearrangements in all ruminant species and would contribute to the understanding of mammalian sex chromosome evolution.

Animals↗
PubMed1995

Physical and linkage mapping of the bovine genome with cosmids.

We have initiated a mapping strategy using cosmid clones to chromosomally anchor a high-resolution bovine genetic linkage map. Ten cosmids containing microsatellites were assigned to bovine chromosomes by fluorescence in situ suppression hybridization (FISH). Four cosmid clones, three of which contain an informative microsatellite, were assigned to autosomes 5, 13, 24, and 28. The assignment to autosome 13 anchors bovine syntenic group U11. Two additional cosmid clones, each containing informative microsatellites, are assigned to autosomes 9 and 29, anchoring bovine linkage groups U2 and U8, respectively. Four cosmid clones, three of which contain informative microsatellites, also provide the first assignment to autosome 25, anchoring bovine syntenic group U7 and orienting the corresponding linkage group relative to the centromere.

Alleles↗
PubMed1995

Combined Q-banding and fluorescence in situ hybridization for the identification of bovine chromosomes 1 to 7.

Eleven probes were assigned to bovine chromosomes 1 to 7 by fluorescence in situ hybridization (FISH). The identification of chromosomes was based on QFQ-banding prior to in situ hybridization and comparison with the Reading Conference (1976) and ISCNDA (1989) standards. The probes used for FISH can now be utilized as identification and discrimination features for bovine chromosomes 1 to 7 and particularly for chromosomes 4 and 6, which are difficult to distinguish. Comparison of our mapping data with previous assignments and of the standard chromosome banding patterns prompt us to propose a change in the ISCNDA nomenclature: ISCNDA chromosome 4 should be named chromosome 6 and vice versa. Chromosome 4 is marked by the ribosomal RNA cluster RNR3, and chromosome 6 is characterized by the casein gene cluster and an anonymous satellite (D6Z1).

Animals↗
PubMed1995

Relationship of restriction fragment length polymorphisms (RFLP) at the bovine calpastatin locus to calpastatin activity and meat tenderness.

Restriction fragment length polymorphisms (RFLP) have been identified at the bovine calpastatin locus. The objective of the present study was to determine whether these polymorphisms are related to variations in calpastatin activity or beef tenderness in unrelated animals of mixed breeding. A sample of 83 crossbred steers from sires representing eight different breeds was examined to determine this relationship. A 2.2-kb cDNA coding for domains 2 through 4 plus a 3' untranslated region of bovine skeletal muscle calpastatin was used as a probe for calpastatin RFLP. Polymorphisms were found using the restriction enzymes BamHI and EcoRI. Polymorphic restriction fragments for BamHI were 9.0 and 5.0 kb and for EcoRI were 6.0 and 4.0 kb. Allelic frequencies for BamHI restriction fragments were .53 for the 9.0-kb allele and .47 for the 5.0-kb allele. Allelic frequencies for EcoRI restriction fragments were .43 for the 6.0-kb allele and >57 for the 4.0-kb allele. No polymorphisms were identified using the restriction enzymes BglII, DraI, or PstI. No associations between EcoRI and BamHI RFLP and 24-h calpastatin activity of Warner-Bratzler shear force at 14 d postmortem were detected. Therefore, the polymorphic EcoRI and BamHI restriction sites within the bovine calpastatin locus do not detect DNA sequence differences responsible for variation in calpastatin activity or tenderness of aged beef. Therefore, these polymorphisms cannot be used to predict tenderness of aged beef from unrelated animals of mixed breeding. These results do not exclude the possibility that other DNA sequences in or near the bovine calpastatin gene are responsible for variation in calpastatin activity or meat tenderness. The lack of a relationship between these calpastatin RFLP and meat tenderness must be distinguished from the well-documented relationship between calpastatin activity and meat tenderness. Therefore, further development of calpastatin-based methods for predicting beef tenderness in unrelated animals of mixed breeding should focus on basic factors influencing calpastatin activity at the molecular and cellular level.

Alleles↗
PubMed1995

Divergent selection for blood serum insulin-like growth factor I concentration in beef cattle: I. Nongenetic effects.

Data were obtained from an ongoing experiment involving divergent selection for blood serum IGF-I concentration in purebred Angus cattle. The experiment includes approximately 100 spring-calving (50 high and 50 low line) and 100 fall-calving (50 high and 50 low line) cows. The selection criterion is the mean of IGF-I concentrations measured on each animal at d 28, 42, and 56 of the postweaning performance test. Mean IGF-I values were available for a total of 526 bull and heifer calves. Pooled across years and breeding seasons, high line progeny averaged 21 +/- 6, 17 +/- 6, and 23 +/- 5 ng/mL more IGF-I (P < .01) than low line progeny at d 28, 42, and 56 of the postweaning test. Season effects on IGF-I approached statistical significance only at d 42 and 56 of the postweaning period. Pooled across years and selection lines, spring minus fall differences were 9 +/- 10 (P = .52), -17 +/- 10 (P = .10), -22 +/- 9 (P = .07), and -9 +/- 6 (P = .45) ng/mL for IGF-I concentrations at d 28, 42, and 56 of the postweaning test and for mean IGF-I, respectively. Sex effects on serum IGF-I concentration were highly significant, with greater IGF-I expression in bulls, but were confounded with diet and location. Age of dam effects were not significant. Regressions of IGF-I concentration on on-test age of calf ranged from .46 +/- .13 to 1.08 +/- .20 ng.mL-1.d-1 and were highly significant for all measures of IGF-I.(ABSTRACT TRUNCATED AT 250 WORDS)

Aging↗
PubMed1994

Relationship of air temperature, relative humidity, precipitation, photoperiod, wind speed and solar radiation with serum insulin-like growth factor I (IGF-I) concentration in Angus beef cattle.

Eight paternal half-sib Angus calves born in late April and early May, 1988 were used to investigate the potential relationship of serum IGF-I concentration with photoperiod and various weather variables including minimum, maximum and average air temperatures, relative humidity, precipitation, wind speed and solar radiation. To determine IGF-I concentration, blood samples were obtained at birth and then weekly until the calves reached 1 mo of age and bi-weekly thereafter. Blood sampling continued until the calves reached puberty as determined by progesterone and testosterone assays. Photoperiod and each weather variable were averaged over the 3 d prior to and including the day of blood sampling (4-d average). Data were divided into two periods: (1) birth through the end of the postweaning period and (2) postweaning period only. Serum IGF-I concentrations were analyzed using a model which included the fixed effects of sex and sample number, the random effect of calf nested within sex and the fixed interaction of sex x sample number, in addition to covariates for weight, photoperiod and weather variables. From birth through the end of the postweaning test, none of the weather variables or photoperiod had significant effects on serum IGF-I concentrations when each was fitted separately. For the postweaning period only, cubic regression coefficients for minimum and average temperatures were .0962 +/- .0325 ng/ml/degrees C3 and .0976 +/- .0272 ng/ml/degrees C3, respectively (P < .01). The quadratic regression coefficient for relative humidity during the postweaning period was -.2991 +/- .1142 ng/ml/%2 (P < .05). The quartic regression coefficient for wind speed during the postweaning period was -36.435 +/- 13.00 ng/ml/(km/hr)4 (P < .01). Maximum temperature, precipitation, solar radiation and photoperiod did not have significant effects on postweaning serum IGF-I concentrations. Based on these data, we conclude that temperature, humidity and wind speed were contributing factors to variation in serum IGF-I concentrations in postweaned beef calves.

Aging↗
PubMed1994

A conceptual database model for genomic research.

We describe a conceptual model for genome databases that facilitates the process of building, maintaining, and disseminating physically anchored genetic linkage maps. The model has been implemented as a relational database at the Roman L. Hruska U.S. Meat Animal Research Center (MARC). Development of consensus maps using disparate data from different reference pedigrees or laboratories is supported. The model is of use to quantitative and population geneticists interested in loci that affect phenotypes and marker-assisted selection, and it is sufficiently flexible for centralized, species genome databases facilitating comparative mapping. The MARC genome database is used to assemble, maintain, and disseminate physically anchored genetic linkage maps for cattle, swine, and sheep currently based on more than 100,000 genotypes from 1,000 markers. Integrated with linkage analysis software, this database permits frequent updates of physically anchored genetic linkage maps.

Algorithms↗
PubMed1994

A genetic linkage map for cattle.

We report the most extensive physically anchored linkage map for cattle produced to date. Three-hundred thirteen genetic markers ordered in 30 linkage groups, anchored to 24 autosomal chromosomes (n = 29), the X and Y chromosomes, four unanchored syntenic groups and two unassigned linkage groups spanning 2464 cM of the bovine genome are summarized. The map also assigns 19 type I loci to specific chromosomes and/or syntenic groups and four cosmid clones containing informative microsatellites to chromosomes 13, 25 and 29 anchoring syntenic groups U11, U7 and U8, respectively. This map provides the skeletal framework prerequisite to development of a comprehensive genetic map for cattle and analysis of economic trait loci (ETL).

Animals↗
PubMed1994

Linkage of bovine erythrocyte antigen loci B, C, L, S, Z, R' and T' and the serum protein loci post-transferrin 2 (PTF 2), vitamin D binding protein (GC) and albumin (ALB) to DNA microsatellite markers.

Seven bovine erythrocyte antigen loci and three serum protein loci were tentatively assigned to chromosomes or synteny groups by linkage analysis to previously assigned microsatellite DNA markers. The erythrocyte antigen locus EAB was mapped to synteny group U27; EAC to chromosome 18, synteny group U9; EAL to chromosome 3, synteny group U6; EAS to chromosome 21, synteny group U4; EAZ to chromosome 10, synteny group U5; EAR' to chromosome 16, synteny group U1; and EAT' to chromosome 19, synteny group U21. The vitamin D binding protein (GC) and albumin (ALB) loci were assigned to chromosome 6, synteny group U15 and post-transferrin 2 (PTF 2) to chromosome 19, synteny group U21.

Animals↗
PubMed1993

Divergent selection for postweaning feed conversion in Angus beef cattle: III. Linear body measurements of progeny.

Postweaning performance data were obtained on 401 group-fed, purebred Angus calves from 24 selected sires (12 high- and 12 low-feed-conversion sires) from 1983 through 1986 at the Northwestern Branch of the Ohio Agricultural Research and Development Center. Body measurements of progeny were taken from 1983 through 1986 for hip height and backfat, whereas others, except chest measurements (depth and width) that were available only in 1985, were taken from 183 progeny born in 1985 and 1986. The objective of this portion of the study was to determine whether differences in linear body measurements exist between progeny of high- and low-feed-conversion sires. Differences between progeny of high- and low-feed-conversion sires tended to exist (P < .10) for heart girth (176.3 +/- 1.2 vs 173.2 +/- 1.2 cm) and were significant for backfat thickness (.94 +/- .01 vs .87 +/- .02 cm). No significant differences were observed for hip height (115.3 +/- .5 vs 115.5 +/- .5 cm), chest depth (58.8 +/- .6 vs 57.4 +/- .6 cm), chest width (44.4 +/- .5 vs 44.0 +/- .5 cm), head width (21.2 +/- .2 vs 21.1 +/- .2 cm), head length (42.4 +/- .3 vs 41.6 +/- .3 cm), muzzle circumference (41.0 +/- .4 vs 41.6 +/- .3 cm), length between hooks and pins (46.7 +/- .5 vs 46.2 +/- .5 cm), length between shoulder and hooks (94.9 +/- 1.0 vs 93.1 +/- 1.0 cm), or volume (361,199 +/- 4,885 vs 352,718 +/- 5,026 cm3).

Animals↗
PubMed1993

Challenges and opportunities for integrating genetically modified animals into traditional animal breeding plans.

Techniques have been developed to introduce specific genes from one species into the germplasm of another, including livestock. This paper reviews reports on evaluation, selection, and breeding procedures for introduction and multiplication of transgenes in breeding populations of livestock. Before transgenes are introduced and multiplied in commercial breeding populations, it is necessary to test transgenics extensively for both favorable and unfavorable transgene effects. Parent stock used to produce transgenic founder animals should be selected to excel in polygenic breeding value for economically important traits, especially if founder animals or their sons are to be used directly by AI in commercial populations. However, polygenic breeding value of founder transgenic animals and inbreeding depression are of negligible importance if a transgene is introgressed into a selection nucleus population from three or four generations of backcrossing. Transgenic development should be economically viable for traits with major effects on net merit, especially in the dairy industry, where transgenic sires can be used extensively by AI. A minimum effect of about 10% of the mean (or one phenotypic standard deviation) is a reasonable approximation of the minimum transgene effect that would be necessary to justify introgression into a nucleus swine population. A transgene effect of 10 to 20% of the mean would be required for most economic traits in beef cattle.

Animals↗