Biomedical subjects
M Cowden
Publications and source records attributed to M Cowden.
Electron diffraction study of hydrated phospholipid single bilayers. Effects of temperature hydration and surface pressure of the "precursor" monolayer.
The molecular packing and phase transition of hydrated dipalmitoylphosphatidylcholine single bilayers are studied by electron diffraction, using an electron microscope equipped with a hydration stage. The phase transition and area per molecule are measured as functions of temperature, hydration and the surface pressure of the monolayer from which the bilayer is formed. The transition temperature of a bilayer agrees with calorimetric measurements on bulk lipid/water mixtures. The molecular packing of a bilayer corresponds to that of the precursor monolyer at a surface pressure of 47 dyne/cm.
Cellular binding of 3H-cytochalasin B.
Explore the source record for details and available documents.
Electron diffraction of wet phospholipid bilayers.
The structure of fully hydrated dipalmitoyl lecithin single bilayers, and monolayers deposited on Formvar substrates are studied by electron diffraction, using a hydration stage fitted to an electron microscope. Selective area diffraction patterns of these films indicate that there are domains consisting of mosaics of crystallites of hexagonally packed lipid chains. The size of these domains are typically several mum in diameter. The diffraction intensity agrees with that calculated from the electron scattering factor of the hydrocarbon chains of the lipid molecule.
Role of cholesterol in membranes. Effects on phospholipid-protein interactions, membrane permeability and enzymatic activity.
Explore the source record for details and available documents.