Biomedical subjects

M Colecchia

Publications and source records attributed to M Colecchia.

23 records · Page 2Linked to original sources

PubMedDate not supplied

[Evaluation of sensitivity and specificity of various staining methods for the detection of myocardial fibrosis].

Fifty bioptic endomyocardial samples from 15 patients with idiopathic dilatative cardiomyopathy have been stained with PicroSirius, Masson-Goldner, Van Gieson Verhoeff, AFOG. The extension of stained areas has been evaluated by morphometry using a videoscan. As control sections samples have been taken from lower third of the right ventricle at autopsies of healthy subjects dying in road accidents. Morphometric analysis of subendocardial interstitial and perivascular fibrosis has been performed. For the authors the most useful stain in the evidentiation of fibrosis was PicroSirius both in the bioptic samples and the autoptic ones.

Adolescent↗

PubMedDate not supplied

[Utility of morphometry and nucleolar organizer regions count in the differentiation of reactive and malignant mesothelial cells in pleural effusions].

The authors have evaluated in eight reactive and eight malignant pleural effusions the number of intranucleolar dots representing the nucleolar associated proteins stained with silver colloid technique (interphase nucleolar organizer regions-AgNOR). The mean number per nucleus in benign reactive mesothelium was significantly lower than in mesothelioma effusions. Other morphometric measurements of nuclear diameters and areas were effected. The authors evaluate the variability of the AgNOR counts and morphology in reactive and mesothelioma cells of pleural fluids.

Humans↗

PubMedDate not supplied

[Meningeal carcinosis: early clinical manifestations of inflammatory cancer of the breast].

Inflammatory carcinoma of the breast accounts for only 1-6% of mammary cancer in Caucasian women and is characterized by a poor prognosis; distant metastases frequently appear in fact in an early stage of disease and moreover metastatic spreading follows unpredictable ways. In this study we report on a case of a female patient in whom persistent signs of increased intracranial pressure, following the diagnosis of inflammatory carcinoma of the breast, have been referable to the tumour seeding the meninges in the absence of systemic disease. This peculiar and unusual form of neoplasia is up today a challenge for the clinician, both because of therapeutic difficulty and of unexpected metastases which, in turn, worsen the prognosis. Particularly, in our opinion, meningeal localization must be suspected even in the absence of distant metastases.

Adenocarcinoma↗

PubMedDate not supplied

[Morphometric evaluation and AgNOR number in c-erbB-2 positive and negative breast carcinoma: absence of correlation].

A total of 722 nuclei from 22 fine needle aspirates (FNA) of breast carcinomas have been subjected to quantitative nuclear analysis. Form Factor, Nuclear Area and Nuclear Major Diameter were determined for each case. An equal number of nuclei from as many aspirates from benign breast nodules was used as control. The aim of the study was to evaluate the predictive value of these parameters on the result of the immunohistochemical determination of the c-erb B-2 antigen in the tumour mass, since 6 of the 22 carcinomas were positive to this antigen. None of these parameters singly or in combination proved of any value in predicting the positive or negative result of the c-erb B-2 antigen determination. Nuclei both positive and negative for the antigen were located within the large cell carcinomas. Nucleolar Organizer Regions were also determined on the histologic sections of both groups to assess any statistically significant difference not only between controls and carcinomas, but also between c-erb B-2 positive and negative cases. Both NOR mean numbers and NOR cytograms were of no use, since no difference was found between c-erb B-2 positive and negative cases.

Breast Neoplasms↗

PubMedDate not supplied

[Nucleolar organizer regions of benign pathology of the parathyroids].

Nucleolar organizer regions (NOR) have DNA loops containing genes that encode for ribosomal RNA; some proteins located near the NORs are argyrophilic and by Ploton's one step method we could quantify the NORs in sections of paraffin embedded tissues. We have evaluated the number of intranuclear dots in 46 parathyroid glands from 18 parathyroidectomies. The glands were weighed and the stromal fat was evaluated by a point-counting method. AgNORs were counted in 100 cells from each gland. The mean count was 3.03 in six adenomas (A), 2.39 in six cases of primary hyperparathyroidism (PH) and 2.63 in six cases of secondary hyperparathyroidism (SH). Statistical analysis performed by Mann-Whitney U test showed that differences between A and PH groups were significant. The difference between A and SH was also significant. The residual parathyroid tissue surrounding the adenoma had a significantly lower AgNOR mean count than adenomatous cells. The AgNOR mean number can be useful in assessing normal or suppressed parathyroid tissue.

Adenoma↗