Search PubMed⌕ Search

Biomedical subjects

M Chvapil

Publications and source records attributed to M Chvapil.

240 records · Page 14Linked to original sources
PubMedDate not supplied

Lanolin and epidermal growth factor in healing of partial-thickness pig wounds.

A total of 80 partial-thickness wounds (4.4 cm2 size, 400 micron deep) was inflicted by electrokeratome in the dermal skin layer of four piglets, 15 kg body weight. The wounds were treated with gauze (control), lanolin cream (Lanolor or Lanolin with emulsifiers, Squibb) or with human epidermal growth factor (EGF) delivered in lanolin cream (10 micrograms EGF/mL cream). The treatment was applied every 12 hours for 12 to 120 hours after wounding. The reepithelization rate of the wound was determined by standardized morphometric method. In addition, we measured the thickness of the dermis and cell counts in the dermis. We found that most of the statistically significant enhancement of the epithelization rate, thickness of the dermis, and higher cell count in the dermis were attributed to the effect of lanolin cream alone. The additional significant enhancement of healing by EGF over that of lanolin alone was documented in one of our experiments, but was only marginal. In another experiment using another commercial formulation of lanolin, we found no difference between the effect of EGF and lanolin. Several hypotheses were suggested to explain the effect of the two tested lanolin cream formulations, which induced strong inflammatory reaction in the wound.

Administration, Topical↗
PubMedDate not supplied

Studies on nonoxynol-9. IV. Biochemical and morphological effects in the liver, kidneys and lungs of rats following intravaginal and intraperitoneal administration.

The content of DNA and collagen was significantly increased in the liver of young adult rats injected intraperitoneally for five consecutive days with Nonoxynol-9 (N-9; 5 mg/100 g). No changes were observed in the lung. A single dose of N-9 increased the activity of SGOT, reaching a maximum 4 to 8 hours after injection. In transmission electron microscopy the hepatocytes showed a dramatic increase of the rough endoplasmic reticulum. Rats injected intravaginally 5 mg/100 g of N-9 daily demonstrated after 15 injections a significant increase of collagen in the liver and kidney. After 15 days, DNA was significantly increased in the kidney. SGOT increased after 5 applications of N-9. Light and electron microscopy of the liver showed signs of nonspecific inflammation. We conclude that N-9 administered to young adult rats intravaginally or intraperitoneally induces biochemical and morphological changes in organs involved in the excretion of this detergent.

Animals↗
PubMedDate not supplied

The importance of liver in normal and silicotic lung-lipid homeostasis. 1. Phospholipids.

We studied the total content, concentration and specific activity of phospholipids (PL) in the lung, liver and serum of rats intratracheally injected with silica or saline. Intact rats served as controls. The animals were sacrificed at four time intervals ranging from 6 to 144 hours. The presented data are based on analyses of 87 rat lungs. Instillation of silica into rat lungs results in a significant increase of PL content in the lung 72 and 144 hours later. These changes, when correlated with PL changes in the serum and liver, indicate that the lung signals the liver to mobilize its PL which are then deposited in the lung. Injections of silica into the lung promote phospholipidgenesis in the liver. The de novo formed PL are then mobilized to the lung via the serum. Saline injection into rat lungs results in increased phospholipidgenesis only in the lung while liver PL content as well as concentration is significantly decreased; however, the synthesis of liver PL is not significantly increased. The lung thus communicates with the liver, causing the liver to respond with phospholipidgenesis and the mobilization of liver phospholipids.

Animals↗