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Biomedical subjects

M Christen

Publications and source records attributed to M Christen.

10 recordsLinked to original sources
PubMed2004

Fast spike pattern detection using the correlation integral.

Conventional approaches to detect patterns in neuronal firing are template based. As the pattern length increases, the number of trial patterns to be tested leads to strongly divergent computational costs. To remedy this problem, we propose a different statistical approach, based on the correlation integral. Applications of our method to model and neuronal data demonstrate its reliability, even in the presence of noise. Additionally, our investigation provides interesting insights into the nature of correlation-integral anomalies.

Action Potentials↗
PubMed2003

The consequences of self-fertilization and outcrossing of the cestode Schistocephalus solidus in its second intermediate host.

Many hermaphroditic parasites reproduce by both cross-fertilization and self-fertilization. To understand the maintenance of such mixed mating systems it is necessary to compare the fitness consequences of the two reproductive modes. This has, however, almost never been done in the context of host-parasite coevolution. Here we show the consequences of outcrossing and selfing in an advanced life-stage of the cestode Schistocephalus solidus, i.e. in its second intermediate host, the three-spined stickleback (Gasterosteus aculeatus). Each juvenile stickleback was simultaneously exposed to 2 experimentally infected copepods, one harbouring outcrossed the other selfed parasites. At 60 days p.i. parasites were removed from the fish's body cavity and, with microsatellite markers, assigned to either outcrossed or selfed origin. Prevalence was not significantly higher in outcrossed parasites. However, those fish that were infected contained significantly more out-crossed than selfed parasites. Thus the probability of a selfed parasite to progress in the life-cycle is reduced in the second intermediate host. Furthermore, we found that even the multiply infected fish increased in weight during the experiment. Nevertheless, total worm weight in multiply infected fish was significantly lower than in singly infected ones, which thus might be a parasite life-history strategy.

Animals↗
PubMed2002

Effects of duodenal fat, protein or mixed-nutrient infusions on epigastric sensations during sustained gastric distension in healthy humans.

Duodenal fat modulates sensory and motor responses to gastric distension and raises plasma cholecystokinin compared with glucose. The effects of protein (also releasing cholecystokinin), or mixed nutrients (with a balanced macronutrient composition), on gastrointestinal sensations in relation to gastric relaxation and plasma cholecystokinin concentrations are not known. The aim of this study was therefore to compare the effects of duodenal infusion of fat, protein or mixed nutrients during sustained gastric distension (mimicking the intragastric presence of food) on these parameters. In 10 healthy subjects, gastric distension to fullness was maintained for 90 min, while gastric volume, sensations and plasma cholecystokinin were monitored during duodenal infusion of isotonic saline or nutrients (2 kcal min-1). During saline infusion, all parameters remained unchanged for 90 min. Initially, only lipid increased plasma cholecystokinin, gastric volume and scores for sensations. Cholecystokinin and gastric volume responses to protein and mixed nutrients were delayed and not associated with significant changes in sensations. In conclusion, the intensity of gastrointestinal sensations is related to, but not entirely explained by, the magnitude in intragastric volume and plasma cholecystokinin changes. Our results offer new insights into the role of dietary nutrient composition in gastrointestinal sensations, and may have implications for the dietary management of digestive symptoms.

Adult↗
PubMed2001

Magnetic field saturation in the Riga dynamo experiment.

After the dynamo experiment in November 1999 [A. Gailitis et al., Phys. Rev. Lett. 84, 4365 (2000)] had shown magnetic field self-excitation in a spiraling liquid metal flow, in a second series of experiments emphasis was placed on the magnetic field saturation regime as the next principal step in the dynamo process. The dependence of the strength of the magnetic field on the rotation rate is studied. Various features of the saturated magnetic field are outlined and possible saturation mechanisms are discussed.

Journal Article↗
PubMed2000

Detection of a flow induced magnetic field eigenmode in the riga dynamo facility

In a closed volume of molten sodium an intense single-vortex-like helical flow has been produced by an outside powered propeller. At a flow rate of 0.67 m(3)/s a slowly growing magnetic field eigenmode was detected. For a slightly lower flow, additional measurements showed a slow decay of this mode. The measured results correspond satisfactorily with numerical predictions for the growth rates and frequencies.

Journal Article↗
PubMed1998

Alteration of fibronectin affinity during differentiation modulates the in vitro migration velocities of Hydra nematocytes.

In the fresh water Cnidarian Hydra nematocytes differentiate from stem cells in the body column of the polyps and are functional in the tentacles to where they migrate as single cells in an amoeboid fashion. The fluorescent vital stain TROMI (tetramethyl-rhodamine-5/6-maleimide) allows to easily discriminate between cells located in the body column and cells mounted in the tentacles. The two cell populations were found to have different in vitro migration properties. These differences appear to be due largely to a differential attachment to fibronectin. Nematocytes from the tentacles show significantly lower in vitro migration velocities on isolated pieces of the organisms extracellular matrix (the mesoglea) and attach more firmly to fibronectin-coated substrates than cells from the body column. Pretreatment of the mesogleae with antibodies against the cell binding domain of fibronectin or addition of RGD-peptides results in an increase of the average migration velocity of cells from the tentacles and a decreased velocity of the cells from the body column. These findings suggest that (1) modulation of the attachment to fibronectin is decisive for the observed differential migration properties of the two cell populations and (2) the in vitro migration of nematocytes is dependent on subtle and transient interactions of cell surface receptors (most probably integrins) and fibronectin.

Animals↗
PubMed1994

Use of capillary affinity electrophoresis for the determination of lectin-sugar interactions.

Capillary affinity electrophoresis was used to study the interactions of lectins from Tetragonolobus purpuras with L-(-)-fucose 1-phosphate. The electrophoretic mobilities of three lectin peaks were influenced by the complexation with the negatively charged fucose derivative. A model that allows the calculation of the complex formation constant from the change in mobility at different concentrations of fucose 1-phosphate has been derived. Calcium ions added to the buffer solution promoted the complex formation of the three lectins. This technique required only minute amounts of the lectins and enabled the simultaneous study of the complex formation of several proteins.

Calcium↗