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Biomedical subjects

M Chivu

Publications and source records attributed to M Chivu.

6 recordsLinked to original sources
PubMedDate not supplied

Detection of human papillomavirus gene sequences in cell lines derived from laryngeal tumors.

The role of Human Papillomaviruses (HPV) in laryngeal carcinomas has been studied with conflicting results. To evaluate the etiologic relationship between HPV infection and epithelial malignancy of the larynx we studied five laryngeal carcinoma cell lines obtained from patients undergoing surgery for laryngeal tumors. The paraffin embedded biopsy samples of the original tumor and different passages of the new established cell lines were investigated by PCR with consensus primers specific for HPV DNA. The findings indicate that HPV infection is associated with some larynx carcinomas. The positive association has been enhanced when a method of enrichment of epithelial cells from fresh tumor samples was used. All tumor cells enriched smears were positive for HPV DNA not only by PCR but also by in situ hybridization (ISH). Investigated by PCR, different passages of larynx tumor cell lines maintained expression of HPV DNA. At subsequent passages ISH gives constantly no signals suggesting a minimal amount of viral harbored sequences. In one cell line propagated more than 60 population doublings, the chromosomal frequency distribution shifted from modal number 46 at the 5(th) passage to 63 at the 60(th) passage. The mechanisms by which persistent HPV infection maintains continuous cell proliferation were discussed.

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PubMedDate not supplied

Metabolic investigations in persistent depressive states in the elderly.

In a group of 100 elderly patients with persistent depressive states, a series of investigations were carried out: hematologic, lipid, protein, energy metabolism hydroelectrolytic and acid-base balance, blood rheologic properties, immunologic, reactivity and certain humoral and regulation mechanisms. The results were compared with those obtained in two control groups, identical in number, the former in the third age without depressive states and the latter of healthy adults. The results were evaluated statistically. The results demonstrate that in depressive elderly subjects changes of the various parameters studied occur in the same direction as those in the group of elderly subjects without depression, but are far more accentuated.

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