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M Chevalier

Publications and source records attributed to M Chevalier.

47 records · Page 3Linked to original sources
PubMedDate not supplied

The mode of presentation and route of administration are critical for the induction of immune responses to p53 and antitumor immunity.

We have examined the immune response to full-length wild-type human p53 presented by a recombinant canarypox vector (ALVAC) and by plasmid DNA. For the ALVAC recombinant, intravenous, but not subcutaneous, intramuscular or intradermal administration, induced CD8+ CTLs that lysed tumor cells transfected with human mutant p53. Intrasplenic administration also induced CTLs. Biodistribution studies showed that intravenously injected ALVAC localized primarily in the lung, liver and spleen, whereas intramuscularly injected virus remained predominantly at the injection site. Intradermal and intramuscular immunization with naked plasmid DNA encoding human wild-type p53 also induced a specific CTL response. DNA immunization induced complete protection against challenge with a mouse embryo fibroblast transfected with human mutant p53 and partial, but significant, protection against a transfected mastocytoma. The ALVAC recombinant induced partial protection in both models. These results suggest that recombinant ALVAC and DNA might be interesting presentation platforms for p53 to be tested in clinical studies.

Animals↗
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Determination of chloramphenicol residues by reverse phase high performance liquid chromatography in foies gras.

A reverse phase liquid chromatographic (LC) method for the determination of chloramphenicol (CAP) residues in foies gras was tested. The method was based on acetonitrile-sodium chloride extraction, sample clean-up with hexane and purification on a Sep-Pak C18 cartridge. Acceptable chromatographic separation was obtained for thawed samples with two sample clean-up steps with hexane, a purification step with acetonitrile + water (87.5 + 12.5) (v/v) before the elution of CAP residues by a mixture of acetonitrile + water (70 + 30) (v/v) with a chromatographic separation on Spherisorb ODS-2 (5 microns) column using sodium acetate buffer (pH 4.3) + acetonitrile (78 + 22) (v/v) as mobile phase. The detection limit of the method was 2.5 micrograms/kg. The mean recovery from spiked samples at the 10 micrograms/kg level was 55.7 +/- 8.7%.

Animals↗
PubMedDate not supplied

[Experimental study on the survival of Vibrio cholerae (El-Tor biotype) in sea-water].

Experimental studies on survival of Vibrio cholerae in untreated sea-water reveal that they remain alive at least one week. A somewhat longer survival (up to 3 weeks) could be observed if these bacteria were kept in sea-water sterilized by filtration. But if sea-water is autoclaved, cholera vibrios may keep their vitality for periods at least 6 months. Our observations lead to the conclusion that untreated sea-water may contains some filtrable thermolabile factor killing cholera vibrios. Yet these facts should not create false feelings of security. It is known, from recent epidemiological data that sea-medium as a whole may be the origin of cholera outbreak.

Seawater↗