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Biomedical subjects

M Cherchi

Publications and source records attributed to M Cherchi.

29 records · Page 2Linked to original sources
PubMed1980

Mouse RBC rosettes in chronic lymphocytic leukaemia: different expression in blood and tissues.

Mouse RBC (M) rosettes were investigated on lymphocytes from peripheral blood (PB) and various tissues in twenty patients with chronic lymphocytic leukaemia and one with follicular lymphoma. In all cases studied, the percentage of M rosettes was significantly higher in PB (median 64%) than in bone marrow (median 15%) and lymph node (median 12%). These differences were statistically significant (P less than 0.01). The possibility that these differences were related to technical factors was ruled out by a number of control procedures. It is suggested that the different expression of M rosette formation reflects the property which determines whether a lymphocyte enters the peripheral blood or remains fixed in the tissues.

Animals↗
PubMed1979

Clinical staging and immunological findings in chronic lymphocytic leukemia.

Several immunological markers were tested in 52 untreated cases of chronic lymphocyte leukemia (CLL) to see whether their frequency differed according to the clinical stage in Rai's system. The leukemic cells in all cases had B-cell features as shown by monoclonal immunoglobulins on the cell surface (SmIg) and/or a high percentage of mouse RBC (M)-rosettes. Of the two B-cell markers, the M-rosette test was the more consistently positive. The frequency of these markers did not correlate with clinical staging. The percentage of T-lymphocytes, low in all cases, was found to correlate inversely with the lymphocyte counts, which were higher in advanced stages. The absolute number of T-lymphocytes was above normal in most cases, but did not relat to staging. At least one of the serum Ig, most commonly IgA, was decreased in 87% of cases. Low Ig were slightly less common in Stages 0-I than in advanced stages (II-IV). The above features were also examined in two groups of CLL patients: with stable (9) or progressive (16) disease. The only difference observed between the two groups was that surface IgM only was present in 1 of the 9 stable cases as compared to 9 of the 16 progressive ones. Our findings do not support the suggestions that surface IgM is a feature of a benign form of CLL or that the absolute number of T-lymphocytes correlates with prognosis.

Adult↗
PubMed1979

'Prolymphocytoid' transformation of chronic lymphocytic leukaemia.

We report clinical, morphological and surface marker studies on seven patients with the common type of chronic lymphocytic leukaemia (CLL) whose disease underwent an insidious though progressive change in character with increasing refractoriness to treatment. This transformation was accompanied by the appearance of a population of immature-appearing cells in the peripheral blood which resembled prolymphocytes, both at light and electron microscopy. The characteristic morphological feature was the presence of two distinct populations of cells, the typical CLL lymphocytes and the 'prolymphocytoid' cells. These cells retained the surface characteristics of CLL, i.e. the information of mouse RBC rosettes and sparse surface-bound immunoglobulin. This transformation can be distinguished by morphological and surface marker criteria from acute leukaemia occurring in CCL, Richter's syndrome and prolymphocytic leukaemia. The recognition of this group of CLL patients may add a new prognostic index to CLL and may help plan subsequent trials for the treatment of the disease.

Aged↗
PubMed1978

Ultrastructural, cytochemical and surface marker analysis of cells during blast crisis of chronic granulocytic leukaemia.

The blast cells of 19 patients with Ph1-positive chronic granulocytic leukaemia (CGL) in blast crisis (BC) were studied by means of several techniques: morphology, cytochemistry, ultrastructure, surface markers and the enzyme terminal transferase. Cells of BC were, in most case, extremely undifferentiated by morphology and cytochemistry. Our data showed that in 80% of cases the cells in BC were myeloid and in 20% they were "lymphoblastic". The M1, M2 and M3 forms (FAB classification) were rare in CGL BC compared with acute myeloid leukaemia (AML). A megakaryoblastic type was seen in 15% of BC cases; the existence of this form could only be demonstrated by electron microscopy. The limphoblastic BC cells were, as in acute lymphoblastic leukaemia (ALL), positive with Greaves' anti-ALL serum and had elevated levels of terminal transferase. A case of a 17-year old boy presenting as ALL, reverting to chronic-phase CGL after complete remission and developing terminally a myeloid BC is described in detail. This case helps to illustrate a new form of natural history of CGL unveiled by the present study.

Adolescent↗
PubMed1978

Acid-phosphatase reaction in acute lymphoblastic leukaemia.

The diagnostic value of the acid-phosphatase reaction was assessed double-blind in 148 cases of acute lymphoblastic leukaemia (A.L.L.) classified by surface-membrane markers and entered into the M.R.C. U.K. A.L.L. trials. 90% of cases of T-A.L.L. showed a positive reaction in the majority of blast cells, while only 2% of common-A.L.L. and 10% of null-A.L.L. were positive. This cytochemical reaction distinguished the more aggressive form of A.L.L. any may aid the choice of therapy.

Acid Phosphatase↗
PubMed1977

Phagocytic potential of leukaemic B-lymphocytes.

Phagocytosis of small latex particles was demonstrated by light and electron microscopy in a proportion of cells from B-lymphoproliferative disorders including chronic lymphocytic, prolymphocytic and hairy cell leukaemia. It was not observed in a case of T-chronic lymphocytic leukaemia or in normal lymphocytes. A significant correlation was observed between the binding of latex to the lymphocyte surface and the degree of phagocytosis. It is suggested that the phagocytic activity of leukaemic B-lymphocytes is mediated through their Fc receptor and that this property may also be present in normal B-cells. Among mononuclear cells, therefore, phagocytosis can no longer be considered exclusively a property of monocytes.

B-Lymphocytes↗
PubMed1976

5' nucleotidase activity in leukaemic lymphocytes.

The enzyme 5' nucleotidase (E.C.3.1.3.5.) is present in lymphocytes isolated from the blood of normal subjects. The activity was extremely low in lymphocytes from 17 patients with B lymphocytic leukaemias. Removal of normal lymphocytes from the B-cell leukaemic samples decreased further the enzyme activity. Moderately low values were observed in three cases of T-ALL and in the cells from a case of Sézary syndrome. In contrast, normal or high values were observed in three cases of ALL in which T and B markers were not demonstrable and in two of T-CLL. No differences were observed in partially purified subpopulations of normal B and T cells.

B-Lymphocytes↗
PubMed1976

Mouse red-cell rosettes in B-lymphoproliferative disorders.

A high proportion of peripheral-blood lymphocytes formed spontaneous rosettes with mouse red cells in 22 out of 23 cases of chronic lymphocytic leukaemia (CLL); the proportion was significantly higher than in 19 cases of other B-lymphoproliferative disorders (non-CLL group) and in 19 normal controls. Intermediate findings were obtained in 10 cases of "hairy" cell leukaemia. Blast cells from various types of acute leukaemia did not bind mouse red cells. Pre-treatment of the lymphocytes with neuraminidase led to a significant increase in the proportion of rosettes in CLL only. This test may prove useful in distinguishing CLL from other B-lymphoproliferative disorders, particularly prolymphocytic leukaemia.

Animals↗