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Biomedical subjects

M Cattoni

Publications and source records attributed to M Cattoni.

At least 19 recordsLinked to original sources
PubMed1975

Ultrastructural studies of human gingiva. V. Microfibrils of elastic nature and their direct penetration of the basal lamina in chronic periodontitis.

In this study, microfibrils widely distributed in the connective tissue of the gingival wall of periodontal pockets were examined by electron microscopy, Theirdistribution, ultrastructural characteristics, close association with fibroblasts and direct communication with the basal lamina were shown. The microfibrils in our specimens were observed in three different groupings (Types A, B, and C). They were believedto represent different stages of maturation of elastic fibera. There was a striking ultrastructural resemblance between oxytalan microfibrils, and the immature or imcompletetype of elastic fiber shown in this report.

Basement Membrane↗
PubMed1972

[Halitosis].

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Halitosis↗
PubMed1968

Electron microscopy of oral cells in vitro. II. Subsurface and intracytoplasmic confronting cisternae in strain KB cells.

Two special areas involving membranous components in strain KB cells were studied by electron microscopy. The first area described is that of the subsurface regions of two apposing cells in which flattened cisternae (one cisternae in each subsurface region) with membranes spaced 110-230 A apart were found in a confrontation alignment. The long dimension of the profiles of these cisternae ranges from 0.5 to 2 micro. At these intercellular contact areas, each cisterna is closely applied to the adjacent plasma membrane; the intervening space is 60-100 A. We have named the cisternae in these roughly symmetrical areas of cell contact the subsurface confronting cisternae. Communications between these cisternae and those of the rough-surfaced endoplasmic reticulum also were observed. The second area described is that of the intracytoplasmic confronting cisternae. These cisternae were observed as oval or round images about 0.3-1.4 micro in diameter, each image being composed of a pair of concentrically arranged confronting cisternae with membranes spaced 200-400 A apart. The apposing membranes of the two confronting cisternae are electron opaque, smooth, and free of ribosomes, whereas the unapposed membranes are less dense, scalloped, and associated with ribosomes. The spacing between the two intracytoplasmic confronting cisternae is 70-110 A.

Cell Membrane↗
PubMed1968

The circumfusion system for multipurposeculture chambers. II. The protracted maintenance of differentiation of fetal and newborn mouse liver in vitro.

The circumfusion system is a complex in vitro pumping unit incorporating 12 multipurpose culture chambers through which a serum-supplemented fluid nutrient is recirculated at a rate of 4.5 ml/min per chamber. This system was used to study the differentiative responses of fetal and newborn mouse liver explants placed in the serum-free environment formed between the sheets of unperforated cellophane and cover glasses of the chambers. Hepatocytes (parenchymal cells) were discernible in 3-5 days. They retained many of their features of differentiation in the circumfusion system for more than 120 days of cultivation. The living morphological characteristics of the hepatocytes were studied by phase-contrast microscopy (direct viewing and time-lapse cinemicrography) and by special cytochemical staining. Electron micrographs were made of both fresh liver specimens and the cultured cells. Comparisons of the cultured parenchymal cells with their in vivo progenitors showed a remarkable preservation of their differentiated state.

Animals↗