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Biomedical subjects

M Carrera

Publications and source records attributed to M Carrera.

At least 73 records · Page 4Linked to original sources
PubMed1991

Quantitative and morphometric analysis of Langerhans cells in non-exposed skin in renal transplant patients.

Renal transplant recipients have a high incidence of cutaneous complications such as neoplasia and viral or fungal infections. Morphologic alterations of epidermal Langerhans cells (LC) have furthermore been described in these patients. Since these changes have been mainly found in sun-exposed skin, a direct effect of immunosuppressive therapy remains a matter of discussion. A quantitative and morphometric study of epidermal LC in non-exposed skin was performed in 28 renal transplant patients (RTP). RTP were divided in two groups according to immunosuppressive treatment: group A; azathioprine + prednisone (14 cases) and group B; cyclosporine + prednisone (14 cases). Twenty sex-age matched non-immunosuppressed patients acted as controls (group C). Epidermal sheets were obtained by incubation in EDTA and stained for ATPase activity and with the monoclonal antibody T6 (CD1) using the avidin-biotin peroxidase method. Langerhans cells were counted using a calibrated graticule (400x) and expressed as the mean number of LC/mm2. The mean area of the LC and the number of primary dendrites (pd) and secondary dendrites (sd) were determined with a morphometer adapted to an Apple II computer. The mean number of positive cells in controls was: ATPase, 677 +/- 157; T6, 695 +/- 164. Patients in group A had the maximum reduction in both ATPase and T6 LC density (ATPase, 339 +/- 142; T6, 402 +/- 194). Patients in group B had an intermediate reduction in the number of LC (ATPase, 494 +/- 121; T6, 529 +/- 112).(ABSTRACT TRUNCATED AT 250 WORDS)

Adenosine Triphosphatases↗
PubMed1988

IgA nephropathy and polycystic kidney disease.

We report a patient with polycystic kidney disease, advanced renal failure, and nephrotic-range proteinuria. Kidney biopsy revealed IgA nephropathy with lesions of focal and segmental glomerular sclerosis. This association had not been previously described and is probably coincidental. This case supports the assumption that the nephrotic-range proteinuria observed in some polycystic patients could be the consequence of another superimposed glomerular disease. This glomerulopathy can worsen the course of azotemia in these patients.

Aged↗
PubMed1988

A simple method for concentrating erythropoietin activity in plasma and in other body fluids.

The method here described is based on the property of erythropoietin to remain in water solution in the presence of up to 60% ethanol. More than 90% of inert proteins precipitate in the process and can be eliminated without significant loss of the activity. The addition of sulfuric ether produces the separation of the water phase and of its solutes which can be recovered in the solid material obtained by lyophilization. Using this method a marked erythropoietin activity can be demonstrated in extracts of the plasma from mice with a moderate reduction of the hematocrit.

Erythropoietin↗
PubMed1986

Sarcoidosis presenting as multiple pulmonary nodules and nephrotic syndrome.

A 56 year old man presented with thrombophlebitis, nephrotic range proteinuria and multiple pulmonary nodules. A renal biopsy showed membranous glomerulonephritis, and after a thoracotomy a diagnosis of sarcoidosis was established. A pulmonary nodular pattern is unusual in sarcoidosis and is often mistaken for malignant disease. The association of this type of pulmonary involvement and membranous glomerulonephritis as the presenting form of sarcoidosis has not previously been described.

Humans↗
PubMed1985

A bioassay method for studying factors stimulating erythropoiesis using as recipient the mouse with a functionally active erythron.

This novel bioassay method basically differs from the conventional plethoric mouse assay in the timing of the onset of induced polycythemia relative to the administration of the substances that are being tested. While the latter measures the regenerating action of erythropoietin at a time when erythropoiesis is virtually absent, the method here described evaluates the stimulatory action(s) required to maintain the normality of the process. This fact opens new approaches for studying factors involved in the quantitative govern of erythropoiesis in the steady state.

Animals↗
PubMed1985

Adrenal myelolipoma in a woman with congenital 17-hydroxylase deficiency.

A case of adrenal myelolipoma, which to our knowledge is the first case to be associated with 17-hydroxylase deficiency, is reported. This rare, benign lesion is known to occur in association with other endocrinopathies. Discussion focuses on the possible role of continued stimulation by corticotropin and/or steroids as pathogenic factors. The present case adds evidence supporting this view.

Adrenal Gland Neoplasms↗