Biomedical subjects

M Carnine

Publications and source records attributed to M Carnine.

7 recordsLinked to original sources

PubMed1999

Characterization of tyrosine sulfate residues in antihemophilic recombinant factor VIII by liquid chromatography electrospray ionization tandem mass spectrometry and amino acid analysis.

Recombinant Factor VIII (rFVIII) is involved in the cascade of biochemical reactions leading to blood coagulation and is used for the treatment of haemophilia A. Plasma-derived FVIII (pdFVIII) has been reported to be post-translationally modified by sulfation of tyrosine residues at positions 346, 1664, 1680, 718, 719 and 723. This report describes the quantitation of tyrosine sulfate residues in BHK-derived, human rFVIII by amino acid composition analysis and the identification of their positions in the polypeptide sequence using a combination of liquid chromatography and electrospray ionization mass spectrometry in the analysis of proteolytic digests of the protein.

Amino Acid Sequence↗

PubMed1990

Liquid chromatographic determination of 2',3'-dideoxyguanosine in human plasma.

A rapid assay for 2',3'-dideoxyguanosine (ddG) in human plasma has been developed. The ddG was first extracted from human plasma using solid phase extraction. The extract containing ddG was then assayed with liquid chromatography using an ODS column and a mobile phase of 9% methanol in pH 6.8 phosphate buffer. The overall method had good accuracy (within 2%), linearity (r = 0.9998), sensitivity (LOD = 1.8 ng, S/N = 3) and recovery (greater than 99% for 5-50 micrograms ddG per ml plasma).

Chromatography, Liquid↗

PubMed1977