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Biomedical subjects

M C Wu

Publications and source records attributed to M C Wu.

At least 127 records · Page 7Linked to original sources
PubMed1988

[Clinical assessment of body hair growth in Japanese women. The relationship between a grade of hirsutism and the menstrual status].

Three hundred and sixty-nine volunteer women and 308 outpatients were studied to clarify the criteria of hirsutism and the relationship between a grade of hirsutism and menstrual status in Japanese women. The body hair growth was assessed according to the modified score system described by Ferriman and Gallway. The menstrual status was classified into six groups based on basal body temperature. The hair growth score calculated in volunteers tended to be lower than that of western women in most sites. Therefore one criterion of hirsutism in Japanese women should be a score of more than 6 points in our system. There was a close correlation between body hair growth and menstrual status. As a result, it can be considered that the systematic assessment of body hair growth is useful in diagnosing and treating some irregular menstrual patients.

Adolescent↗
PubMed1987

Purification and characterization of human colony-stimulating factor 1 from human pancreatic carcinoma (MIA PaCa-2) cells.

Colony-stimulating factor 1 (CSF-1) was purified from the serum-free conditioned medium of a human pancreatic carcinoma cell line (MIA PaCa-2) by a combination of conventional chromatography and high-performance liquid chromatography. The purity of human CSF-1 was demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with a diffuse single band of Mr 42,000-50,000 and by N-terminal amino acid analysis of glutamate residue. The CSF-1 was stable at 50 degrees C for 30 min. It is sensitive to treatment with trypsin, chymotrypsin, and subtilisin but less sensitive to papain digestion. Treatment of CSF-1 with different glycosidases did not affect the biological activity. Sulfhydryl reagents such as dithiothreitol (DTT), iodoacetic acid, and N-ethylmaleimide did not affect the biological activity at the concentration of 1 mM. However, CSF-1 activity was inhibited totally by the combination of 10 mM DTT and 1 mM SDS. Under denaturing and reducing conditions, CSF-1 appeared on SDS-PAGE as a single protein band of Mr 21,000-25,000 and concurrently lost its activity, indicating that human CSF-1 possibly consists of two similar subunits and that the intact quaternary structure is essential for the biological activity. When treated with neuraminidase and endo-beta-D-N-acetylglucosaminidase D, the molecular weight of CSF-1 was reduced to 36,000-40,000, and to 18,000-20,000 in the presence of mercaptoethanol. Because of the specificity of endo-beta-D-N-acetylglucosaminidase D, it is suggested that the carbohydrate moieties are Asn-linked "complex-type" units.

Biological Assay↗
PubMed1987

Differentiation of cultured promyelocytic leukemia cells (HL-60) induced by endotoxin-treated human lung conditioned medium.

Serum-free conditioned medium prepared from endotoxin-treated human lung tissue contains potent differentiation activity (DA) which induces the differentiation of human promyelocytic leukemia cells (HL-60) to mature functioning granulocytes and macrophages. Upon fractionation, the DA can be separated from colony-stimulating factors (CSFs). The estimated molecular weight of DA is 23,000 d which is similar to CSF-II but is much smaller than CSF-I (50,000 d). The isoelectric point (PI) value obtained from preparative isoelectrofocusing is 5.2, which is higher than CSF-I of 4.1-4.8 but lower than CSF-II of 5.6. Using isopropanol as solvent and trifluoroacetic acid (0.2%) as ion-pair, the DA is eluted at 37% isopropanol from the C-3 column in comparison to 30% for CSF-II and 34% for CSF-I.

Cell Differentiation↗
PubMed1987

Relationships between uterine length and number of fetuses and prenatal mortality in pigs.

Relationships between the length of uterine horn and number of fetuses and prenatal mortality were characterized in 320 pregnant pigs at 3, 5, 7, 9, 11, 13 and 15 wk of gestation in a cross-sectional design. Genital tracts of all pregnant animals available on the days of collection were measured. Length of each uterine horn, numbers of fetuses and corpora lutea (CL) were recorded and prenatal mortality was calculated. With each additional fetus, length of the uterus increased 10 cm regardless of stage of gestation (P less than .001). The association of number of fetuses and uterine length was local and confined to that horn in which the fetus resided and did not extend to the opposite horn. As number of CL increased, number of fetuses also increased as did prenatal mortality. There was a significantly negative correlation between uterine length and prenatal mortality when animals were classified into four groups on the basis of number of CL; less than 10, 10 to 14, 15 to 18 and greater than 18. Results indicated that the number of fetuses and prenatal mortality were closely correlated with length of the uterus. Length of the uterus appeared to be an important limiting factor to litter size as number of CL increased.

Animals↗
PubMed1986

Induced rapid phospholipid methylation and arachidonic acid release by dimethyl sulfoxide-treated human promyelocytic leukemic HL-60 cells.

The incubation of undifferentiated promyelocytic HL-60 cells with DMSO resulted in the rapid transmethylation of phosphatidyl ethanolamine (PE) into phosphatidylcholine (PC) which was maximal at 60 secs. This rapid generation of PC was followed by a decrease of the methylated phospholipid and the release of arachidonic acid. Thus, the rapid DMSO-induced phospholipid methylation coupled with release of arachidonic acid (precursor for eicosanoids) prior to morphological evidence of cellular differentiation may represent early biochemical events which result in the generation of intracellular chemical signals which may program the promyelocytic cells into a differentiation mode.

Arachidonic Acid↗
PubMed1986

Determination of serum delta 5-3 beta-hydroxysteroid sulphates by combined high-performance liquid chromatography and immobilized 3 beta,17 beta-hydroxysteroid dehydrogenase in column form.

We studied the use of an immobilized enzyme, covalently bound to aminopropyl-CPG, in the analysis of individual delta 5-3 beta-hydroxysteroid sulphates. A microcolumn with immobilized 3 beta,17 beta-hydroxysteroid dehydrogenase was prepared and used together with high-performance liquid chromatography (HPLC). The reduced nicotinamide-adenine dinucleotide produced from delta 5-3 beta-hydroxysteroids by this enzyme was fluorimetrically determined. The immobilized enzyme was sufficiently stable for at least one month or for 180 tests when used repeatedly. A clinical trial demonstrated that this HPLC-immobilized enzyme method is superior to the soluble enzyme method, giving reliable and reproducible results at a low cost.

Adult↗
PubMed1986

Modulation of colony stimulating factor-induced murine myeloid colony formation by S-peptido-lipoxygenase products.

Prompted by the observation that the selective blockade of lipoxygenase pathway by known inhibitors of this pathway inhibited the colony stimulating factor (CSF)-induced colony formation in both mouse and human marrow cells and the fact that this inhibitory effect could be reversed by a crude total lipoxygenase extract from an incubation of rat peritoneal neutrophils and arachidonic acid, we investigated which of the two major classes of lipoxygenase products, mono- and dihydroxy fatty acids (5S-hydroxyeicosatetraenoic acid and 5S-, 12R-dihydroxyeicosatetraenoic acid) and the S-peptidohydroxy fatty acids (leukotrienes C4 and D4), is involved in the modulation of CSF-induced colony formation by mouse marrow cells. Data from incubations with synthetic lipoxygenase products revealed that (a) the culture of mouse marrow cells in the presence of varying amounts of purified CSF-I (Mia PaCa 2) with a low concentration (5 micrograms/ml) of nordihydroguaretic acid consistently suppressed CSF-induced colony formation by approximately 40-50%. This concentration of nordihydroguaretic acid selectively inhibits only the lipoxygenase pathway; (b) additions of two S-peptido-hydroxy fatty acids [5S-hydroxy-6R-S-glutathionyl-7,9,11,14-(E,E,Z,Z)-eicosatetraenoic acid and 5S-hydroxy-6R-S-cysteinglycyl-7,9,11,14-(E,E,Z,Z)-eicosatetraenoic acid] to these partially suppressed colonies restored the CSF-induced colony formation to control levels in a dose-dependent manner; and (c) additions of two mono- and dihydroxy acids, 5S-hydroxyeicosatetraenoic acid and 5S-12R-dihydroxyeicosatetraenoic acid [5S, 12R-dihydroxy-6,8,10,14-(Z,E,E,Z)-eicosatetraenoic acid], failed to restore the nordihydroguaretic acid inhibited colony formation. These novel observations underscore the importance of these peptido-5-hydroxy fatty acids and the lipoxygenase pathway in the mechanism of CSF-induced colony formation in mouse marrow cells.

Animals↗
PubMed1986

Lipoxygenation of arachidonic acid by differentiated and undifferentiated human promyelocytic HL-60 cells.

The human promyelocytic leukemia cell line HL-60 can be induced to differentiate into mature granulocytes by exposure to dimethyl sulfoxide (DMSO), as well as into cells of monocyte-macrophage lineage by exposure to 12-0-tetra-decanoylphorbol-13-acetate (TPA). Incubation of the promyelocytic leukemic HL-60 cells and the two chemically induced differentiated cells with carbon 14-labeled arachidonic acid overnight resulted in the incorporation of label mainly into cellular phospholipids. Stimulation of the labeled cells with Ca2+ ionophore (A23187) resulted in the generation of lipoxygenase products in all the cells. Notably, the DMSO-induced differentiated granulocytic cells biosynthesized predominantly the peptidoeicosatetraenoic acids LTC4 and LTD4 compared with the dihydroxyeicosatetraenoic acid LTB4. On the other hand, the TPA-induced differentiated monocytic-macrophage cells biosynthesized predominantly LTB4 compared with LTC4 and LTD4. The undifferentiated promyelocytic cells lacked the capacity to biosynthesize LTB4, as evidenced in the chemically induced differentiated cells. The monohydroxyeicosatetraenoic acid (HETE) 5-HETE, a metabolite of the 5-lipoxygenase pathway, was a minor component in these cells, and 12-HETE and 15-HETE were barely detectable. These results suggest that the two models of DMSO-induced and TPA-induced differentiated cells may be useful systems for further investigations of arachidonic acid metabolites in granulocytic and monocytic-macrophage functions.

Arachidonic Acid↗