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Biomedical subjects

M C Liberto

Publications and source records attributed to M C Liberto.

24 records · Page 2Linked to original sources
PubMed1986

X-linkage of the early in vitro alpha/beta interferon response of mouse peritoneal macrophages to herpes simplex virus type 2.

The genetics of the early interferon response of mouse peritoneal cells to infection with herpes simplex virus type 2 (HSV-2) was studied in susceptible BALB/c and more resistant C57BL/6 mice and in reciprocal crosses between these mice. Wash-outs of the peritoneal cavity of normal C57BL/6 mice contained significantly more cells than wash-outs from BALB/c mice. Therefore, interferon induction with HSV-2 was studied under standardized conditions in vitro. Peritoneal cells reacted to HSV-2 infection by interferon production in a virus dose-dependent manner. Interferon was detected first after 2 h and peaked after 24 h. Cells from C57BL/6 mice of each sex produced significantly more early interferon than cells from BALB/c mice, and cells from female BALB/c mice produced more interferon than cells from males. This difference was not seen with C57BL/6 mice. Cultures of highly purified adherent cells yielded approximately 10 times as much interferon as cultures of non-adherent cells. Since treatment of cells with carbonyl iron and silica significantly reduced the amount of interferon produced, whereas 2000 rad of irradiation had no obvious effect, it is concluded that the main interferon-producing cell in the peritoneal cavity of mice in response to HSV-2 is of the monocyte/macrophage lineage. Interferon production in peritoneal cells was found to be quantitatively influenced by X-linked loci in that cells from male (BALB/c female X C57 male) F1 mice, which inherit the X chromosome from the low-responding BALB/c females, produced significantly lower amounts of interferon than cells from the other three F1 generation genotypes. All interferons were characterized as alpha/beta interferon. It is suggested that the early production of alpha/beta interferon in response to HSV-2 is influenced by X-linked loci, which might be involved in sex-linked differences in resistance to human herpesviruses.

Animals↗
PubMed1985

The restoration of impaired macrophage functions using as immunomodulator the Corynebacterium granulosum-derived P40 fraction.

Many microorganisms and compounds of microbial origin exhibit immunomodulatory activities and have been extensively used in immunotherapy of experimental animal tumors and in patients with neoplasia. In this paper we describe the effect of the C. granulosum-derived P40 fraction on the growth and metastatization of the transplantable epithelioma T8 of Guèrin. Moreover, we evaluated the effect of P40 treatment on several depressed macrophage functions of tumor-bearing rats. In particular, the phagocytic and chemotactic activities of such cells were studied, as well as the antiviral intrinsic and extrinsic activities against HSV-1 and the anti-Toxoplasma gondii activity. All these functions were depressed in untreated tumor-bearing rats. Administration of a single intravenous injection of P40 fraction led to the restoration of all depressed macrophage activities to normal values. In particular, the possibility of restoring the antimicrobial activity of macrophages from tumor-bearing rats by treatment with this immunomodulator is of great concern when one considers the increasing incidence of opportunistic infections in immunocompromised hosts. Results are discussed in terms of both the possible mechanism of action of P40 and of its possible target cells.

Adjuvants, Immunologic↗
PubMedDate not supplied

Impairment of macrophage antiviral activity by soluble tumor products. Effects of bacterial immunomodulators.

The antiviral activities of normal rat peritoneal macrophages versus Herpes Simplex Virus type 1 were inhibited by sera from tumor-bearing rats and 3M KCl extracts of tumor mass. The inhibitory activity was demonstrated on the extrinsic as well as on the intrinsic macrophage functions. Sera from Corynebacterium parvum and Listeria monocytogenes treated tumor bearing rats did not inhibit these macrophages functions. Furthermore the 3 M KCl extracts from the tumor mass of the above treated TBR show a decrease in the capability to impair these macrophage functions. On the other hand, the treatment with the oral polyvalent adjuvant "Buccalin" was not able to restore the compromised antiviral activity in tumor bearing rats.

Adjuvants, Immunologic↗
PubMedDate not supplied

"In vitro" study on human mononuclear phagocytic cells behaviour versus DNA and RNA viruses.

It is generally accepted that mononuclear phagocytic cells are of crucial importance in defence against viral infections. Using a method of "in vitro" differentiation from human blood monocytes to macrophages, the interactions between human monocyte-macrophage with herpes simplex virus type 1 and 2, adenovirus type 17, coxsackie virus B type 4, poliovirus type 1 and ECHO virus type 2, are reported. In all viruses tested on monocytes, the infection of the cells was very poor according to the data of specific immunofluorescence and of the residual infectivity. In the macrophage stage all viruses tested were able to infect the cells.

DNA Viruses↗