Biomedical subjects

M C Berlinghieri

Publications and source records attributed to M C Berlinghieri.

25 records · Page 2Linked to original sources

PubMed1985

Effect of oral administration of different combinations of killed bacteria on some depressed macrophage functions in tumor-bearing rats.

In the present study, we compared the ability of different bacterial species administered orally in various combinations to restore some depressed peritoneal macrophage functions in tumor-bearing rats. Phagocytosis, killing of Candida albicans and chemotactic response of resident peritoneal cells from treated tumor-bearing rats were influenced by different associations of bacteria. In particular, when Staphylococcus aureus was administered together with other bacterial species, the phagocytic activity of peritoneal cells was restored to normal values, and intracellular killing of C. albicans was enhanced. The results are discussed in relation to the possible influence of mucosal bacterial flora on the level of activation of peritoneal macrophages. The possibility that bacterial species can influence in various ways immunocompetent cells in relation to the different chemical composition of some common structures is also discussed.

Administration, Oral↗

PubMed1985

The restoration of impaired macrophage functions using as immunomodulator the Corynebacterium granulosum-derived P40 fraction.

Many microorganisms and compounds of microbial origin exhibit immunomodulatory activities and have been extensively used in immunotherapy of experimental animal tumors and in patients with neoplasia. In this paper we describe the effect of the C. granulosum-derived P40 fraction on the growth and metastatization of the transplantable epithelioma T8 of Guèrin. Moreover, we evaluated the effect of P40 treatment on several depressed macrophage functions of tumor-bearing rats. In particular, the phagocytic and chemotactic activities of such cells were studied, as well as the antiviral intrinsic and extrinsic activities against HSV-1 and the anti-Toxoplasma gondii activity. All these functions were depressed in untreated tumor-bearing rats. Administration of a single intravenous injection of P40 fraction led to the restoration of all depressed macrophage activities to normal values. In particular, the possibility of restoring the antimicrobial activity of macrophages from tumor-bearing rats by treatment with this immunomodulator is of great concern when one considers the increasing incidence of opportunistic infections in immunocompromised hosts. Results are discussed in terms of both the possible mechanism of action of P40 and of its possible target cells.

Adjuvants, Immunologic↗

PubMed1985

Effects of a new quinoline derivative, ciprofloxacin, on some professional phagocytic cell functions.

This study reports the effects of ciprofloxacin on in vitro chemotactic, phagocytic and bactericidal activity of human polymorphonuclear leukocytes, human mononuclear cells differentiated in vitro and rat peritoneal macrophages. After in vitro preincubation with different concentrations of ciprofloxacin, acridine orange vital staining for phagocytosis and killing and the Boyden chamber for chemotaxis evaluation were used. Results obtained with different cell types showed no negative effects on the different functions tested, even at concentrations ranging from about 100 micrograms/ml.

Animals↗

PubMed1984

Inhibition of normal rat macrophage functions by soluble tumor products. Effect of systemic treatment with bacterial immunomodulators.

The phagocytic and chemotactic activities of normal rat peritoneal macrophages were inhibited by sera from tumor-bearing rats (TBR) and 3 M KCl extracts of tumor mass. However, sera from Corynebacterium parvum- or Listeria monocytogenes-treated TBR did not inhibit phagocytosis. On the other hand, sera from C. parvum-treated, but not from L. monocytogenes-treated TBR still inhibited the chemotactic response of the normal macrophages. Furthermore, 3 M KCl extracts of tumors from C. parvum-treated TBR did not inhibit phagocytosis and chemotactic response of the same cells. Similar results were obtained with extracts of tumor masses from L. monocytogenes-treated rats. It is suggested that treatment with bacterial immunomodulators can influence the release from neoplastic cells of soluble products influencing normal macrophage functions.

Adjuvants, Immunologic↗

PubMedDate not supplied

Different effects of bacterial lipopolysaccharide on superoxide anion production by macrophages from normal and tumor-bearing rats.

Bacterial endotoxins or lipopolysaccharides (LPS) exhibit a wide range of modulatory activities on immunocompetent cells. Among the numerous effects of LPS on macrophages, an enhancement of superoxide anion (O2-) release has been reported. In previous studies carried out on tumor-bearing rats, it was found that several functions of peritoneal macrophages such as phagocytic, microbicidal and antiviral activities were depressed. In this paper we evaluated the spontaneous or phorbol myristate acetate (PMA)-induced production of superoxide anion by macrophages from tumor-bearing rats with respect to controls. Moreover, the effect of in vitro priming with LPS on O2- production by the same cells was studied. It was found that the pattern of superoxide release by macrophages from tumor-bearing rats is significantly different from controls. Preincubation of macrophages from normal rats with LPS enhanced the spontaneous and PMA-induced production of O2-. In contrast, the same concentrations of LPS did not prime macrophages from tumor-bearing rats.

Animals↗

PubMedDate not supplied

Effect of oral administration of a variety of bacteria on depressed macrophage functions in tumour-bearing rats.

In consideration of the well documented influence of normal microbial flora on the level of activation of macrophages, we evaluated the effects of oral administration of bacteria on rats with depressed macrophage functions. An oral, killed polyvalent vaccine (Diplococcus pneumoniae types I, II and III, Streptococcus haemolyticus, Staphylococcus aureus and Haemophilus influenzae), the live lyophilized Streptococcus faecium and spores of Bacillus subtilis, respectively, were administered orally to immuno-depressed rats. Results demonstrate the restoration of phagocytosis, intracellular killing and the chemotactic activity of macrophages. These experimental observations suggest that bacterial flora associated with mucosae can influence the level of activation of peritoneal macrophages.

Administration, Oral↗

PubMedDate not supplied

Human mononuclear phagocytic cell interaction with some Paramyxoviridae.

In consideration of the peculiar relationship between Paramyxoviridae and immunocompetent system, we studied interactions of measles virus, Newcastle-disease virus and respiratory syncytial virus with human monocytes and macrophages differentiated "in vitro". Mononuclear cells were challenged with three different Paramyxovirus at different multiplicities of infection. Infected cells were then checked for expression of specific viral antigens by immunofluorescent test (FA). Moreover, we have evaluated the intrinsic antiviral activity and single cycle growth curves.

Cell Differentiation↗