Biomedical subjects
Publications and source records attributed to M C Barrett.
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Senescent rats were administered ethanol by gastric intubation at hourly intervals. After 1 h, there was a rapid increase in the specific activities of two isoenzymes of liver mitochondrial aldehyde dehydrogenase which was sustained for the next 2 h. No further changes were observed during the course of the following 2 h.
Brief exposure of rat liver mitochondria to hypo-osmotic sucrose media caused a decline in the rates of succinate and ascorbate-NNN'N'-tetramethyl-p-phenylenediamine oxidation without loss of cytochrome c when assayed in iso-osmotic media. Lowered respiration rates in mitochondria after brief exposure to hypo-osmotic media may reflect a modification of cytochrome c binding.
Aldehyde dehydrogenase activities in liver mitochondria isolated from rats given ethanol at hourly intervals by gastric intubation showed a brief lag period followed by a rapid increase in specific activities until a maximum was attained at about 3h.
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Rat liver mitochondria treated extensively with n-pentane are incapable of oxidizing choline. Choline oxidation is more sensitive than is succinate oxidation to serial n-pentane extraction of mitochondria. The ability to oxidize choline is restored by the addition of ubiquinone-2 or ubiquinone-10 to the oxidase assay medium.
1. The choline dehydrogenase (EC 1.1.99.1) WAS SOLUBILIZED FROM ACETONE-DRIED POWDERS OF RAT LIVER MITOCHONDRIA BY TREATMENT WITH Naja naja venom. 2. The kinetics of the reaction of enzyme with phenazine methosulphate and ubiquinone-2 as electron acceptors were investigated. 3. With both electron acceptors the reaction mechanism appears to involve a free, modified-enzyme intermediate. 4. With some electron acceptors the maximum velocity of the reaction is independent of the nature of the acceptor. With phenazine methosulphate and ubiquinone-2 as acceptors the Km value for choline is also independent of the nature of the acceptor molecule. 5. The mechanism of the Triton X-100-solubilized enzyme is apparently the smae as that for the snake venom solubilized enzyme.
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This study showed that nonsearchers and searchers are different from each other on almost every measure that was applied. Research on and experience with searching adult adoptees should not be applied to nonsearching adult adoptees. Likewise, research on and experience with nonsearching adult adoptees should not be generalized to searchers . The results of this study do not support the belief that adoptees, in general, have low self-concepts and identity conflicts, or that adoptees need information about their biological families and reunions to resolve their identity conflicts. Although the sample of pre- and post-reunion searchers was small, reunions did not make a significant difference in self-concept or identity conflicts, as measured by the TSCS . The results of this study do not support the theoretical bases often cited to justify open records. Specifically, adult adoptees in this study do not have negative self-concepts, did not experience poor adoptor - adoptee relationships, and the majority did not experience revelation of adoptive status as disruptive or late. Although significant differences exist between the comparison groups, an important finding is that of all the adult adoptees, the majority scored above the sixtieth percentile on the TSCS and had positive scores on the Attitude Toward Parents Scales. Furthermore, they were happy growing up, with only 12% reporting being unhappy. These findings support the conclusions of Day [1979] and Norvell and Guy [1977] as well as the belief that adoption is a legitimate way of building families.(ABSTRACT TRUNCATED AT 250 WORDS)