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Biomedical subjects

M Butler

Publications and source records attributed to M Butler.

At least 181 records · Page 10Linked to original sources
PubMed1975

Amino acids attached to transfer ribonucleic acid in vivo.

1. tRNA was extracted from rabbit liver by both the phenol and diethyl pyrocarbonate methods under conditions preventing deacylation of the amino acids attached in vivo. 2. After deacylation 12 amino acids were determined by gas-liquid chromatography, by using the flame-ionization and nitrogen-sensitive thermionic detectors. 3. Comparison of the distribution of 12 amino acids attached to tRNA with those contained in total tissue protein and in the free pool showed little correlation. 4. Results for the enzymic charging assay for tRNA in vitro did not correlate satisfactorily with the analysis of amino acids attached to tRNA in vivo. Marked differences were ntoed in comparison made between our own and other published results.

Amino Acids↗
PubMed1975

Assimilation of citric acid and adipic acid by the blue-green alga Anabaena variabilis.

The assimilation of [1,6-14C] citric acid and [1,6-14C] adipic acid by the blue-green alga Anabaena variabius was studied in the dark and in the light. Citric acid was assimilated in the dark and in the light but adipic acid showed only limited assimilation in the dark. In the light the assimilation of adipic acid did not enhance the growth of the alga at a concentration of 2.85 X 10(-8) M. Growth was inhibited at adipic acid concentrations greater than 10(-3) M. Analysis of the products of adipic acid metabolism showed the presence of aspartic acid, glutamic acid, leucine, proline, and threonine and threonine amongst other unidentified compounds. A mechanism of beta-oxidation is proposed.

Adipates↗
PubMed1975

Studies on the prostate glands of adult inbred LSH hamsters.

We have studied several parameters of prostate function in two inbred lines (2.4 and SsLak) of young and old LSH hamsters. These included weight, acid phosphatase, and [3H]testosterone uptake as influenced by age, castration, and androgen treatment. In the hamster, prostatic acid phosphatase concentration was found to vary inversely with androgen levels, contrary to the usual assumption that this enzyme is androgen dependent. Prostatic uptake of tritiated testosterone was enhanced by castration and by treatment of castrates with doses of androgen which induced a moderate increase in gland size. With advancing age, the prostates of LSH hamsters (both strains) became atrophic rather than hyperplastic, in contrast with a previous report (1). This atrophy appeared to be a consequence of decreased testicular function. The LSH hamsters appear to be a suitable model for the study of senescent changes in the male reproductive system.

Acid Phosphatase↗
PubMed1974

Flat gel polyacrylamide electrophoresis of porcine mycoplasmas.

The flat gel acrylamide electrophoresis technique was standardized and applied to the comparison of four species of porcine mycoplasmas. Clear differences were observed between these species, and differences were seen among the strains of Mycoplasma hyosynoviae. The clarity of the patterns and the number of bands developed was influenced by the amount of protein in the extract and the age of the culture. The technique allows the comparison of several protein extracts in parallel without the problems associated with the rearrangement of separate gel columns.

Animals↗
PubMed1972

An unusual plaque variant of rubella virus.

In a comparative study of 24 different rubella virus strains, all but three formed small plaques in RK 13 cell cultures; of these one was the vaccine strain HPV-77, one was isolated from a congenitally infected infant, and the third was recovered from an adult who contracted severe rubella while handling this congenital strain. Whereas the plaque size of the vaccine strain was stable after further passage in cell culture, the plaque size of the other two rapidly diminished when the virus was passed in monkey kidney cells, and one of them was also reduced by passage in RK 13 cells. Cell culture passage of the typical small plaque strains did not result in altered plaque size.

Adult↗