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Biomedical subjects

M Bussi

Publications and source records attributed to M Bussi.

81 records · Page 5Linked to original sources
PubMedDate not supplied

Evidence of suppressor activity mediated by concanavalin-A stimulated cells towards aspecific LIF production.

Since the T-suppressor subpopulation is known to have a peculiar sensitivity to the mitogenic activity of Concanavalin-A we decided to verify whether in normal subjects it was possible to induce, by using the Con-A as a mitogenic factor, the proliferation of cells--possibly T-suppressor--which: a) had the property of adherence and b) show inhibitory activity toward LIF production. The statistical analysis of the results of our experimental patterns show that even if a difference between the suppression exerted on LIF production by Con-A stimulated versus not stimulated cells is present at any dilution, the level of significance rises from P less than 0.005 to P less than 0.0001 for the range of dilution 1:1/4 to 1:132, thus revealing that these experimental conditions are optimal for eliciting the blocking effect. The researches that are currently in progress in our laboratory seem to suggest that this experimental approach is of some value in characterizing the immunity pattern of laryngeal cancer patients and its evolution through the course of disease.

Cell Adhesion↗
PubMedDate not supplied

Evaluation of the immune responsiveness in laryngeal cancer patients with specific and aspecific tests: first results of follow-up in 80 cases.

We have evaluated in 80 patients suffering with laryngeal carcinoma their specific and aspecific immune responsiveness. We used the E rosette-test, the PHA-induced in vitro stimulation and the in vitro specific LIF production. We chose to use the LIF production test both for its marked sensitivity and because the lymphokines--which are soluble mediators--act as a "bridge" between the different fields of the immune response. Furthermore in our previous research, we observed that the specific LIF production was influenced in a negative way by adherent cells of the peripheral blood that could behave as blocking factors. For what attains the autologous LIF production, before and after the removal of adherent cells, our results show that the patients suffering with a cancer staged as T3T4N1N2N3 exhibit much more marked blocking phenomena than the patients with a cancer staged as T1T2T3N0. In fact the results demonstrate that in this second group only 62% of the cases, after the removal of the adherent cells, showed an increase of LIF production, while this percentage rose to 83% for the patients with a more advanced disease. A similar pattern was found in those patients who showed a recurrence two of three years after the primary disease.

Humans↗
PubMedDate not supplied

[Long-term survival following chemotherapy for recurrent head and neck cancer: patient characteristics].

Eighty-one patients with recurrent squamous cell carcinoma of the head and neck (60 patients) or undifferentiated carcinoma of the nasopharyngeal type (21 patients) were treated with cisplatin (80-100 mg/m2iv d1) and 5-fluorouracil (750-1000 mg/m2Cl d1-5). In squamous cell carcinoma patients there were 7 complete responses (11.6%), 25 partial responses (41.7%), 20 stable disease situations (33.3%) and 8 progressions (13.3%). In nasopharyngeal carcinoma patients there were 6 complete responses (28.6%), 9 partial responses (42.8%), 3 stable disease situations (14.3%) and 3 progression (14.3%). There were 5/60 patients (8.3%) with disease-free survival > 18 months in the squamous cell carcinoma group and 2/21 (9%) in the nasopharyngeal carcinoma group. All long-term survival patients had the following characteristics: good performance status (0-1 ECOG scale), disease- free interval from the first treatment > 12 months, local non-bulky relapse (rT1-2). A re-irradiation with palliative doses (20-48 Gy) was conducted in 3/7 patients. This treatment may have improved the response to drugs with regard to length and quality.

Adult↗
PubMedDate not supplied

[Effects of total laryngectomy on the quality of life: a study on certain psychological aspects].

The importance of certain psychological aspects during post-surgical rehabilitation of laringectomized patients is being more and more emphasized in research on the subject. The aim of this study is to consider a group of 55 patients who had undergone a total laryngectomy 6 to 48 months previously. The patients studied spoke using an esophageal voice and were chosen from among a group of 400 operated in our Department. This investigation was carried out not only from an organic point of view but took into consideration the consequence of such an important disability in the personal psychological, social, domestic and relational sphere as well. The study was carried out through a partially guided interview whose aim was to obtain anamnestic information regarding psychological symptoms present at the moment of the interview, the amount of understanding of the patients and their relatives concerning the diagnosis and the signs of sinergy. The Zung scale for self-evaluation of depression and the STAI scale for state and trait anxiety were employed. Through a careful scrutiny of the data, it appeared that the quality of life was the main problem, above all because such a problem is an inevitable consequence of lost vocal functionality. We found that the overall average score of the Zung scale was 31.6% (A patient who obtains a score higher than 40% is considered depressed). Twelve patients among those we examined were above this threshold level. The score of the STAI scale partially overlapped those obtained by a control group made up of patients suffering from anxiety disorders.(ABSTRACT TRUNCATED AT 250 WORDS)

Adaptation, Psychological↗
PubMedDate not supplied

[Histological and immunohistochemical aspects of mucosal-epidermal junction following surgical reconstruction of upper aero-digestive tract].

As a preliminary of the study of the possibility of upper aerodigestive tract reconstruction using "myomucosal" flaps derived by the classical pectoralis major myocutaneous flap, the evaluation of histologic and immunohistochemical modifications of epidermidis implanted in oral cavity and oropharynx has been conducted in 10 patients. The patients were operated for cancer of oral cavity and oropharynx and then reconstructed using the pectoralis major flap and biopsies from the mucosal-cutaneous junction were taken at different times from operation. Histologically, epidermidis preserved its morphology (a keratinized layer thinner but still present at 24 months by the operation, all the cutaneous annexes). A progressive modification of basal characteristics at the cutaneous side and rapid repair of the basal membrane were observed both histologically and immunohistochemically. In addition, a rich inflammatory infiltrate was present in the derma of the cutaneous area. Histochemistry demonstrated that mucosa and skin keep their own cytokeratin production although they repair in the absence of a fibrotic scar.

Basement Membrane↗