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Biomedical subjects

M Burger

Publications and source records attributed to M Burger.

At least 91 records · Page 5Linked to original sources
PubMed1977

A serum-free medium for the Mishell-Dutton system.

A serum-free medium which provides high recoveries of plaque-forming cells in the primary immune response to sheep erythrocytes is described. In this medium the serum was replaced by heated fetuin and by excess of nonessential amino acids. Glycopeptides, obtained by excessive pronase treatment of fetuin were partially capable of replacing the nonhydrolyzed fetuin.

Amino Acids↗
PubMed1977

Marked instability of character of cloned epithelial cells.

Cell lines derived from 7,12-dimethylbenzanthracene-induced rat mammary carcinomas show a marked drift in properties when cultured in vitro. The following characteristics alter progressively with the number of passages: cloning efficiency, requirement for hydrocortisone, requirement for fetal sera, growth response to conditioning factor(s) and ease of detachment from the culture vessel by trypsinization.

9,10-Dimethyl-1,2-benzanthracene↗
PubMed1977

Stimulation of the in vitro immune response by factors present in the human serum.

Two distinct factors which stimulate the recovery of plaque-forming cells in the vitro immune response have been found in human serum. One acts in the presence of fetuin and the other augments the recovery of plaque-forming cells in its absence. The molecular weight of the former is higher than 1 X 10(5). The two factors could be separated from each other by ammonium sulphate fractionation. Large differences were found in the amount of these factors in sera obtained from different human donors.

Ammonium Sulfate↗
PubMed1971

Accumulation of untranslated lactose-specific messenger ribonucleic acid during catabolite repression in Escherichia coli.

When Escherichia coli K-12 Hfr.H was induced to synthesize beta-galactosidase in the presence of glucose, an untranslated lactose-specific mRNA (lac-mRNA), protected from decay, was found to accumulate progressively within the cells. The lac-mRNA accumulation was unaffected by the carbon source on which the cells had been grown before the induction. The amount of the lac-mRNA available for translation was affected by catabolite repression and 3':5'-cyclic AMP, but it remained unclear whether this was a direct effect on the formation of the lac-mRNA or a consequence of the effect on its translation.

Acridines↗