Search PubMed⌕ Search

Biomedical subjects

M Budai

Publications and source records attributed to M Budai.

16 recordsLinked to original sources
PubMed2004

Studies on molecular interactions between nalidixic acid and liposomes.

The interaction between nalidixic acid sodium salt (NANa) and liposomes prepared from alpha-L-dipalmitoyl-phosphatidylcholine (DPPC) or from its binary mixture with dioleoyl-phosphatidylcholine (DOPC) was studied with differential scanning calorimetry (DSC) and electron paramagnetic resonance (EPR) spectroscopy. We evaluated the role of broadband ultraviolet-B (UV-B) irradiation on the molecular interactions between the lipids and the NANa, and determined the decay-kinetics of the incorporated spin labeled fatty-acid free radicals. Multilamellar and unilamellar vesicles were prepared by sonication and extrusion. The entrapment efficiencies were determined spectrophotometrically. The size-distribution of the liposomes and its change in time was checked by dynamic light scattering (DLS). Our results indicate that NANa mainly interacts with lipid head groups. However, its effect and presumably the formation of the free radicals, induced by broadband ultraviolet-B, is not localized only to the head group region of the lipid molecules. Depending on DOPC content, interaction between the NANa and the lipids modifies the phase-transition parameters of the liposome dispersions.

1,2-Dipalmitoylphosphatidylcholine↗
PubMed2003

Molecular interactions between DPPC and morphine derivatives: a DSC and EPR study.

The interaction between different morphine derivatives (morphine, codeine, N-methyl-morphine, N-methyl-codeine) and alpha-L-dipalmitoyl phosphatidylcholine (DPPC) liposomes was studied with differential scanning calorimetry (DSC) and electron paramagnetic resonance (EPR) spectroscopy. Small unilamellar DPPC-liposomes with the given morphine-derivative were prepared by sonication. The size distribution of liposomes was checked by dynamic light scattering (DLS). The amount of entrapped morphine was determined spectrophotometrically. Our results indicate that the morphine and its derivatives principally interact with the lipid head groups, and this interaction leads to a decrease in the mobility of the polar head groups, especially in case of codeine and N-methyl-codeine.

1,2-Dipalmitoylphosphatidylcholine↗
PubMed2001

[Caries, gingivitis and dental abnormalities in patients with cleft lip and palate].

Oral health was studied in 31 children aged 10-12 years with cleft lip and palate and compared to 31 noncleft controls matched for sex and age. We found a difference in the prevalence and activity of caries with increase in cleft lip and palate patients. The unilateral crossbite and the congenitally missing teeth were more common in cleft palate children and the supernumerary teeth were fewer. Almost all children used fluoride tablets.

Case-Control Studies↗
PubMed2001

[Liposomes as drug carrier systems. Preparation, classification and therapeutic advantages of liposomes].

Bangham et al. (1965) created first the concept of the liposome as a microparticulate lipoidal vesicle separated from its aqueous environment by one or more lipid bilayers. Later Gregoriadis and Ryman (1972) suggested to use liposomes as drug carrier systems. Nowadays liposomes are under extensive investigation for improving the delivery of therapeutic agents, enzymes, vaccines and genetic materials. Liposomes offer an excellent opportunity to selective targeting of drugs which is expected to optimize the pharmacokinetical parameters, the pharmacological effect and to reduce the toxicity of the encapsulated drugs. To understand the system it is important to know the basic properties of these lipoidal vesicles. Our aim was to focus on the lipid composition and the method of liposome preparation what determine the liposomal membrane fluidity, permeability, vesicle size, charge density, steric hindrance and stability of the liposomes as principle factors those influence the fate of liposomes, their interactions with the blood components and other tissues after systemic administration or local use.

Drug Carriers↗
PubMed2001

[Preparations of liposomal morphine. Investigation of the interaction of morphine and its derivatives with the dipalmitoyl phosphatidylcholine membrane].

Liposomes composed of the lipids of biological membranes are suitable for drug delivery. To reach a better therapeutical effect it is important to know the properties of interactions between the drug and lipid molecules. From dipalmitoyl phosphatidylcholine (DPPC) using ultrasound small liposomes containing morphine were prepared. The amount of entrapped morphine was determined with spectrophotometry and luminescence spectroscopy. The interaction between the molecules of morphine and its derivates (codeine, N-methyl-morphine, N-methyl-codeine) and the DPPC lipid was studied with differential scanning calorimetry (DSC) and electron spin resonance (ESR) methods. Our studies indicated that the molecules of morphine and its derivates principally interact with the environment of DPPC lipid head groups. Due to the interaction the mobility of head groups decreases especially in case of codeine and N-methylcodeine.

1,2-Dipalmitoylphosphatidylcholine↗
PubMed1991

[Open bite and its management].

Morphological and functional symptoms of various varients of the open bite are reported on. The orthodontic treatment possibilities and, then, the surgical methods applicable after termination of jaw bone development are summarized. The functional disturbances have to be considered in case of both the conservative and the surgical treatment. A patient treated with a fixed device and an operated patient are presented.

Adolescent↗
PubMedDate not supplied

Experimental study on the effects of +Gz. Acceleration under gestational conditions. I. Ultrastructural myocardial lesions.

In the course of a complex experimental study concerning the effects of highly positive acceleration on gestation and development of the neonate, investigations were carried out of ultrastuctural myocardial lesions in the pregnant rat exposed to prolonged gravitational stress (+ 2G). The experimental animals presented various disseminated and partly irreversible myocardial lesions whose pathogeny is discussed.

Animals↗