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Biomedical subjects

M Bruni

Publications and source records attributed to M Bruni.

34 records · Page 2Linked to original sources
PubMed1978

Changes in the lung alveolar proteins in some experimental conditions.

The Authors have directed their research to the alveolar lining (the surfactant) proteins and they have particularly studied its alterations in shock pathological conditions. Some rats have been subjected to haemorrhagic and toxic shock, by Escherichia Coli's lipopolysaccharide; then lung washing has been performed and on extracted liquid proteins have been studied by two-dimensional immunoelectrophoresis (TDI). The obtained data have been encouraging and very useful for studies process.

Animals↗
PubMed1978

The proteins of the lung alveolar lining.

The protein composition of the lung washing has been analysed by immunochemical methods: it has been possible to demonstrate the presence of many proteins, mainly albumin, immunoglobulins and beta-globulins, some of them seem laying in the alveolar lining and having the function of stabilizing the surfactant system.

Animals↗
PubMedDate not supplied

[Ovarian thyroid].

Explore the source record for details and available documents.

Adolescent↗
PubMedDate not supplied

[The presence of asbestos on board ships: optical microscopy as a research instrument].

Identification of asbestos in many different kinds of bulk materials was performed by means of optical microscopy and the results are reported in the present paper. Some hundreds of various samples taken aboard ships were analysed: panels, laggings, spray insulations, etc. These analyses were required to be not only reliable but also rapid. The results demonstrated the high reliability as well as the rapidity of the technique. Using both the well-known dispersion staining technique (with central stop objectives or with dark field condensers) and phase-contrast analyses on the same polarizing microscope, we carried out numerous checks on the optical properties of the fibres. Not only were dispersion staining colours detected but also refractive index, elongation and extinction signs, in order to obtain an absolutely certain identification. The Italian laws which deal with the asbestos detection discourage the use of the optical microscope because of its presumed unreliability. This paper tends to demonstrate that Italian laws underestimate the potential of the optical microscope. Optical microscopy is probably the only technique that is reliable, inexpensive and rapid at the same time.

Asbestos↗
PubMedDate not supplied

An alternative method of detecting dispersion staining colors for the determination of asbestos fibers in bulk materials.

Asbestiform mineral particles can be detected and identified by means of a polarized light microscope, making use of the dispersion staining technique. In order to obtain dispersion staining (central stop), che rays which pass undeviated through liquid and specimen must be stopped. A microscope objective can be fitted with a device which can insert a central stop on the objective back focal plane to prevent undeviated rays from reaching the objective. Alternatively, the sample can be observed in a conventional dark field set-up, that is, it can be illuminated by a hollow cone of light of greater aperture than the microscope objective so that only rays which are strongly deviated can enter the objective. The first method, proposed by McCrone and widely used in the USA, allows very bright and defined colors to be obtained but at the same time entails low resolution. The second method, recommended by italian regulations as a method that can be used in asbestos characterization, produces less defined and fainter colors. By suitably modifying the angular aperture of the illuminating cone and regulating the distance between condenser and specimen, good results were achieved: on the one hand a good image resolution were retained, on the other, the colors observed were bright and well defined. The detection of these colors can represent a fundamental step in the characterization of the specimen under analysis.

Asbestos↗