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Biomedical subjects

M Bressel

Publications and source records attributed to M Bressel.

At least 37 records · Page 2Linked to original sources
PubMed1986

[Determination of steroid receptors in prostate cancer: possibilities and limits].

In most cases, the prostatic carcinoma (PCA) is a hormone-dependent malignant tumor. Since sexual steroids seem to act upon their target organs by means of specific steroid receptors the determination of receptor concentration in PCA is of great theoretical and practical interest. The state of art of receptor research in PCA can be summarized as follows: 1. The prognostic relevance of receptor determinations in PCA is controversial. The contradictory assessments of the value of receptor measurements may be mainly caused by methodical problems. 2. A possible heterogeneity of the receptor distribution in PCA tissue can not be detected by biochemical receptor determinations. 3. This heterogeneity could be one reason for a lack of response to endocrine therapy in cases which are biochemically receptor-rich (selection of receptor-poor cell clones). 4. The available (immuno)histochemical fluorescence techniques, which have been developed for direct detection of heterogeneous receptor distribution in the tissue, are neither sufficiently sensitive nor specific. 5. It is an open question whether the development of an appropriate (immuno)histochemical method will succeed.

Humans↗
PubMed1984

Differential calcium response of normal and adenomatous parathyroid glands.

In vitro parathyroid hormone secretion of normal (n = 10) and adenomatous (n = 20) human parathyroid glands was compared in response to different calcium (Ca++) concentrations. The glands, prepared for tissue culture immediately after surgical removal, were incubated under identical conditions for 6 h. The medium was changed hourly and analyzed for PTH and cAMP using radioimmunoassay. During the first 2 h of the experiment, the Ca++ concentrations of all preparations was kept constant at 1.2 mM equivalent to the normal Ca++ level of the intercellular space. The PTH level of the 2nd h was defined as reference value corresponding to 100%. After the 2nd h of incubation the Ca++ concentration in the medium was shifted either to low (0.9 or 0.6 mM) or to high (1.9 or 2.6 mM) values. In low Ca++ concentrations (0.6 mM) the normal parathyroid glands responded by stimulation of the PTH release up to 310% in relation to the reference value, whereas the adenomas enhanced the PTH release to 160% only. The incubations in 0.9 mM Ca++ resulted in a slightly lower degree of stimulation. During the incubation in high Ca++ the PTH secretion was reduced to 28% by normal glands and to 52% by adenomatous parathyroid glands. Movements of cAMP measurements paralleled PTH values. The study provides evidence for an abnormally low responsiveness of parathyroid adenomas to Ca++ when compared with normal glands. The reduction in Ca++ responsiveness of adenomatous cells appears to have a fundamental role in primary hyperparathyroidism since the tumour cells may obviously recognize normal Ca++ levels as 'hypocalcaemic' and react by a stimulated PTH secretion.

Adenoma↗
PubMed1980

[The incidence of regional lymph node metastases in operable prostatic carcinoma (author's transl)].

Operation specimens of 165 radical prostatectomies were examined systematically for their pathological anatomy. In 22 cases (13.3%) there were metastases in the obturator and (or) iliac lymph nodes, in 12 cases these were micro-metastases with a maximal diameter of 2 mm. Ten were classified as N1, 7 ad N2 and 5 as N4. Lymph node involvement increased with increasing tumour staging: from 10% in T2 to 26.3% in T3. All metastasizing tumours had invaded the capsule (P3), 77% had additional infiltration of the seminal vesicles and in 73% the tumour volume was at least 50% that of the prostate. Thus prostate carcinoma must reach a certain size and penetrate the capsule before lymph node seeding occurs. The rate of metastases increases ninefold when the seminal vesicles are infiltrated. All metastasizing prostate cancers were polymorphic and in 73% the tumour differentiation in the metastases was identical. The findings as regards localisation of the tumour in the prostate and the metastases correlated in 91%. This result leads one to suspect a regular course of laterally localised spread in which the group of obturator lymph nodes appears to lie in the primary lymph drainage area of the prostate. The prognostic significance of regional lymph node metastases cannot yet be evaluated.

Aged↗
PubMed1980

[Possibilities for the evaluation of receptor sites in benign prostatic hyperplasia and carcinoma of the prostate (author's transl)].

Cytoplasmic and nuclear receptor sites of the prostate will be determined for a qualitative information about the pattern of specific bindings. Their qualitative evaluation and their selective suppression will give an idea about the hormonal influence and regulation in prostatic tissue. These investigations are performed in some cases of prostatic cancer. The difficulty of tissue preparation is caused by the individual distribution of collagen tissue in prostatic cancer which will decide the yield of purified receptor sites. Besides the elemination of unspecific binding sites like SHBG and albumin is an evidence for the value of a method. According to high amount of binding sites the most effective preparation of patients before a tissue biopsy is discussed. For the clinical application the DCC test in case of cytoplasmic sites and the exchange test for nuclear sites seem to provide a suitable method. Performing gelfiltration and additional test with denatured material is necessary because of the similar size of SHBG and receptor complexes interfering the results.

Binding Sites↗
PubMed1978

[Diagnosis of primary hyperparathyroidism based on determination of parathormone in venous blood of the neck (author's transl)].

Experiences with 77 patients with primary hyperparathyroidism (HPT) are reported. Among the diagnostic parameters, the serum calcium level is the most significant; a definite diagnosis can be made through PTH-RIA. The problem of HPT diagnosis are discussed. For standardization, our own human PTH preparation, produced from tissue culture of operatively removed human adenoma of the parathyroid gland, has been used. For determination of parathormone, venous blood should be selectively extracted from the neck before every relapse-necessitated operation. The technically expensive and difficult examination methods do not excuse the surgeon from carefully exploring all of the parathyroid glands, though the general procedures to be applied before the first operation are still disputed.

Antigen-Antibody Complex↗
PubMed1976

Carcinoma and dysplastic lesions of the prostate. A histomorphological analysis of 50 total prostatectomies by step-section technique.

50 prostate carcinomas which were totally prostatectomized together with removal of the seminal vesicles in all cases and pelvic lymphadenectomy in 38 cases were studied histologically. The material was cut by step-section technique in 5 mm thick slices and "large area slides" were made. 4 of the 50 carcinomas were morphologically circumscribed (stage I), 6 tumors were limited to the organ (stage II) and 40 prostate carcinomas had already penetrated the capsule, i.e. fascia of Denonvillier (stage III). In 12 cases the seminal vesicles were involved, regional lymph node metastases were seen 8 times. The carcinomas were mainly localized in the peripheral part of the organ (28 X in the periphery, 21 X both peripherally and centrally and only 1 X in the centre). Multifocal tumor growth was found in 30 cases (60%). The main mass of tumor was mostly situated in the middle (25 X) and caudal (15 X) zone of the prostate. During the course of tumor growth the expansion was directed centrally but then mainly longitudinal and parallel to the urethra. By progressing tumor volume there was a noticeable increase in capsular penetration as well as infiltration of the seminal vesicles and lymph node metastases. Histologically 10 carcinomas showed a uniform pattern, a unique solid and/or cribriform tumor architecture was never observed. 90% of the pluriform carcinomas consisted of the morphological stage III.

Adenocarcinoma↗
PubMed1976

[Surgical treatment of erectile impotence using the AMS penile prosthesis].

First experiences with an AMS penile prosthesis which can hydraulically fill and empty is reported. Advantage of this prosthesis: Physiologic imitation of erection and handling this method. The best cosmetic solution of all previous types of penile prosthesis. Disadvantage: A relatively complicated system and thus liable to defects. However, repairing is, in any case, easily done. After 3 years of use, experience is showing that this method turned out to be a cosmetically and functionally satisfying operative procedure.

Erectile Dysfunction↗