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Biomedical subjects

M Bravo

Publications and source records attributed to M Bravo.

At least 109 records · Page 6Linked to original sources

[The Diagnostic Interview Schedule in Spanish: its translation and adaptation in Puerto Rico].

The process and results of the Puerto Rico's DIS Spanish translation and adaptation are described in this paper. The process is presented in the context of cross cultural research on psychiatric diagnoses and the translation and adaptation of diagnostic instruments for use across cultural boundaries. Several translation techniques were employed (bilingual committee, back-translation and instrument testing) to obtain an instrument in correct Spanish, comprehensible for most Spanish-speaking people as well as linguistically and culturally adapted to the Puerto Rican population. Its semantic, technical, contents and criterion equivalence with the English DIS were considered. The resulting instrument is not only a translation and adaptation of the original but it also presents some additional features which aim to enhance its diagnostic accuracy. A descriptive and quantitative comparison with the Karno et al's Spanish DIS version is also included as well as a discussion of its possible contribution to DIS transcultural use, specially in Hispanic cultures.

Cross-Cultural Comparison↗

Effect of epinephrine and alprenolol on ethanol metabolism, liver cell respiration and mitochondrial function.

Chronic administration of epinephrine to adult male rats resulted in a significant increase in the rate of ethanol elimination, when given alone or together with the beta-adrenergic blocker alprenolol. This effect was observed concomitantly with an increased hepatic oxygen utilization and no changes in mitochondrial respiratory functions. Epinephrine given acutely did not modify the rate of ethanol metabolism. Blood glucose levels were enhanced in these conditions, but were unaffected in rats treated with epinephrine plus alprenolol. These results suggest that chronic epinephrine treatment induces an increased oxidative capacity in the liver characterized by enhanced rates of oxygen uptake and ethanol metabolism, which is not related to its beta-adrenergic actions.

Alprenolol↗

[Metabolic regulation of nutrition].

The mechanisms regulating nutrient metabolism and their adaptation and compensation during stress conditions are discussed. The stress conditions considered are fasting and refeeding, pregnancy, growth and development, and malnutrition.

Carbohydrate Metabolism↗

The significance of cytochrome c redistribution during the subcellular fractionation of rat liver.

1. The redistribution of mitochondrial cytochrome c during homogenization and subcellular fractionation of the liver was studied. Chromatographically homogeneous (14)C-labelled cytochrome c was added in different amounts to liver suspensions immediately before homogenization and the adsorption of radioactivity was determined in cytochrome c fractions extracted at pH4.0, first with water and then with 0.15m-sodium chloride. 2. The soluble cytochrome c remaining in the cell sap after subcellular fractionation was 7% of the calculated amount of cytochrome c passing through a soluble form during the whole process. The total amount of cytochrome c released in a soluble form and subsequently redistributed was 25-30% of the total liver cytochrome c. 3. In the standard microsomal fraction the cytochrome c extracted with water originated entirely from redistribution whereas that extracted with 0.15m-sodium chloride was 80% endogenous. In the mitochondrial fraction both cytochrome c pools were truly endogenous, so that practically none of the mitochondrial cytochrome c released to the soluble cell sap was readsorbed by the mitochondria. 4. These results support our former hypothesis that the cytochrome c extracted with 0.15m-sodium chloride at pH4.0 from the standard microsomes represents the cytochrome c newly synthesized in situ, since it does not originate from redistribution. However, the microsomal pool extracted with water cannot be an intermediate in the postulated transfer of cytochrome c from the microsomal particles to the mitochondria, since this pool arises from redistribution of mitochondrial cytochrome c.

Animals↗