Search PubMed⌕ Search

Biomedical subjects

M Boda

Publications and source records attributed to M Boda.

12 recordsLinked to original sources
PubMed1999

The caffeine metabolic ratio as an index of xanthine oxidase activity in clinically active and silent celiac patients.

BACKGROUND: The xanthine oxidoreductase system has been identified as one of the main sources of free radicals responsible for various forms of tissue injury. Because the intestinal villi are an important location of this enzyme, it was of interest to study the role of xanthine oxidase in gluten-sensitive celiac enteropathy, associated with characteristic villous atrophy. Measured by a noninvasive method, the ratio of caffeine metabolites excreted in the urine after a caffeine challenge had previously been shown to be indicative of the total xanthine oxidase activity of the patient. METHODS: The study involved 22 children with gluten-challenged celiac disease, exhibiting subtotal villous atrophy in specimens from the third intestinal biopsy in accordance with ESPGHAN criteria. Ten of the patients displayed overt clinical symptoms (active form), whereas 12 had no symptoms (silent form). Urinary caffeine metabolites were determined by high-pressure liquid chromatography. The total in vivo xanthine oxidase activity was expressed as the caffeine metabolite index. RESULTS: In patients with active celiac disease the xanthine oxidase activity index was considerably higher, whereas in those with silent disease it was significantly lower than the control value. A significant negative correlation was shown between the index indicative of xanthine oxidase activity and the serum iron level of the patients. CONCLUSIONS: Activation of xanthine oxidase may play a role in the pathogenesis of active celiac disease with definite malabsorption, gastrointestinal symptoms, and anemia. The caffeine test reflects the difference in the pathogenetic mechanism leading to the mucosal lesion and clinical symptoms of active and silent forms of celiac disease.

Caffeine↗
PubMed1998

Coeliac disease: always something to discover.

The authors present more than 20 years' experience with coeliac disease, with a summary of their published studies. Hair shaft characteristics were determined by scanning electron microscopy. Hair diameter was significantly lower and cuticular erosion scores higher in those who were not on gluten-free diets as compared to controls, showing a tendency towards normal values following start of gluten-free diets. Proton-induced X-ray emission showed significantly lower zinc content of the hair shaft in the group with acute coeliac disease and after a short-term diet, which approached the normal range only after a year-long diet. The serum prolactin levels in healthy controls and in coeliac patients on the diet were within normal limits, whereas in children with coeliac disease taking gluten in their meals, a significant hyperprolactinaemia was found. The erythrocyte glutathione content of coeliac children was elevated, and the glutathione disulfide level was significantly decreased, as compared to values in normal controls. The erythrocyte glutathione disulfide level and glutathione disulfide/erythrocyte glutathione ratio in coeliac children also differed from those in children with iron deficiency. With genotyping, the DQB1*0201/2 (p < 0.00001) and DR3 (p < 0.00001), DR7 (p < 0.01) alleles showed significant positive association with the disease.

Anemia, Iron-Deficiency↗
PubMed1992

Determination of hair trace elements in childhood celiac disease and in cystic fibrosis.

Applying proton-induced X-ray emission authors investigated the hair trace element contents in 10 children with acute celiac disease after 3 to 6 and 12 months long gluten-free diet; in 9 children with cystic fibrosis and in a control group (6 children) of the same age. There was no difference in Cu, Fe, Ca, Cl values between the examined groups. The Zn contents of the hair are significantly low in the group with acute celiac disease after a short-term diet and also in the group with cystic fibrosis, the data approach the normal range only after a year's diet. The significant rise of hair potassium contents is well indicated in patients with acute celiac disease and this rise may be due to the destruction of cell cuticles. In case of cystic fibrosis there is no significant rise of hair potassium value.

Calcium↗
PubMed1992

Decrease in the antioxidant capacity of red blood cells in children with celiac disease.

The erythrocyte glutathione metabolism of 11 children with acute celiac disease (CD), 11 children under gluten free diet with CD and 5 children with cow's milk allergy was compared to that of 11 children with nutritive iron deficiency and to 22 healthy children as controls. Erythrocyte glutathione (GSH) content of celiac children was elevated and the glutathione disulfide (GSSG) level was significantly decreased as compared to normal controls. Erythrocyte GSSG/GSH ratio in acute CD differed also from the one in iron deficiency. In vitro oxidative load of acetylphenylhydrazine proved the impaired glutathione stability of the erythrocytes in acute CD and cow's milk allergy. A parallel rise of methemoglobin and hemichrome level of blood cells was seen. Further on, the selenium content of the red blood cells of CD patients decreased. All alterations of the erythrocyte tended to normalize during the dietetic period. These data suggest a reduced protective capacity of erythrocytes in CD and in cow's milk allergy in childhood against oxidizing stresses.

Celiac Disease↗
PubMed1989

[Selenium levels in erythrocytes of children with celiac disease].

Selenium is the component of the glutationperoxidase one of the most important enzymes in the antioxidant protection of the organism. In preliminary studies the authors found in celiac children increased sensitivity to oxidative stress and biochemical changes referring to decreased activity of glutationperoxidase enzyme. Therefore the selenium content of washed erythrocytes has been determined with fluorimetry in 24 celiac children kept on gluten containing diet and 25 on gluten-free diet. In gluten loading the selenium level was significantly lower than in the healthy control group. The selenium value rose at a small degree in the erythrocytes of celiac children kept on gluten-free diet but did not reach the lower limit of the physiological value. On the basis of these results the malabsorption of selenium was found to play also an important role in the decreased protecting capacity against oxidative stress effects in celiac diseases of childhood. The necessity of supplementing the trace element is raised.

Celiac Disease↗
PubMed1983

The importance of cow's milk protein intolerance in chronic diarrhoea of children.

A survey is given of the cases diagnosed as cow's milk protein intolerance in the last 5 years. The diagnosis was established on the basis of the regression of clinical symptoms after elimination from the diet of cow's milk and their recurrence after milk challenge. In more than half of the cases intestinal biopsy was carried out; three patients were rebiopsied after milk challenge. Intestinal biopsy is indicated solely in cases when the exclusion of coeliac disease is necessary for the correct diagnosis.

Absorption↗
PubMed1981

[Incorporation of H3 uridine in chick embryo skin cultured in media containing histones].

In this paper the authors have carried out further research on the differentiation of six-day limb skin from chick embryos cultured in vitro in mediam containing histones. When 3H-uridine is added to this modified medium, a decrease of 3H-uridine uptake and incorporation is observed. The authors conclude that in their system the histones not only interfere with the regulation of genetic transcription by inhibiting DNA-dependent RNA synthesis, but they also change cellular permeability.

Animals↗
PubMed1979

Renal tubular ferritin-uptake, a consequence of the increased glomerular permeability, during the benign and malignant course of renal hypertension in rats.

Tubular uptake of ferritin given intravenously was studied in the right and left kidneys of 74 Goldblatt-hypertensive rats. Previous observations pointed out the pathologically enhanced permeability of glomerular barrier as the cause of the phenomenon. It was assumed, that the extent of tubular areas taking up ferritin, refers to the number of damaged glomeruli. The process was characterized semiquantitatively by planimetric measurements and determination of the non-hemin iron concentration in the renal cortical tissue. A more frequent and extensive tubular ferritin-uptake (and glomerular damage) was bilaterally recorded in the kidneys of malignant hypertensive rats in comparison to the benign ones. The development of the phenomenon in the clamped kidneys, being defended from high blood pressure, suggests a humoral factor behind the enhanced glomerular permeability. Saline intake has a beneficial effect on the glomerular damage similar to the hypertensive angiopathy.

Animals↗