Biomedical subjects

M Bier

Publications and source records attributed to M Bier.

47 records · Page 3Linked to original sources

PubMed1976

Serum protein fractionation by isotachophoresis using amino acid spacers.

Analytical and preparative isotachophoresis has been carried out using amino acids and peptides as discrete spacers in contrast to the usually employed continuous mobility spectrum Ampholine. Analytical isotachoresis in free solution, using the LKB Tachophor, demonstrated the separation of human serum into distinct mobility subgroups, n spacers giving rise to n + 1 protein subgroups. Preparative fractionation on polyacrylamide gel was carried out on the LKB Uniphor using threonine and glycine as spacers. Immunoelectrophoretic analysis showed that eight out of ten proteins assayed were clearly resolved in the three subgroups obtained, thus demonstrating the sharpness of isotachophoretic resolution.

Amino Acids↗

PubMed1975

Hypochlorite-induced alterations to canine serum complement.

Changes in the concentration of the components of complement produced by NaOC1 both in vitro and in vivo are recorded. C1, C4 and C7 are particularly sensitive to this oxidizing agent, although all components decrease at high concentrations of NaOC1. Following oxidation, complement componenets return rapidly to normal. Data are presented to indicate that part of this repair mechanism is due to the action of reducing agents such as ascorbic acid and part is due to the synthesis of the individual components. The unique sensitivity of complement components to oxidation make this treatment of potential value in suppressing the inflammatory response.

Animals↗

PubMed1973