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Biomedical subjects

M Bibbo

Publications and source records attributed to M Bibbo.

209 records · Page 12Linked to original sources
PubMedDate not supplied

Chromatin appearance in intermediate cells from patients with uterine cancer.

This paper presents the differences found by the computer analysis of digitized images of visually apparently normal intermediate cells from patients with normal cytology and patients with uterine cancer. There exist differences in the absolute amount of nuclear staining, the average density of staining, cell shape and size and also differences in spectral contrasts of the nuclear chromatin. Visual cytologic detection of these changes became more conspicuous when the photomicrographs were arranged in order of their discriminant function scores.

Carcinoma in Situ↗
PubMedDate not supplied

Cytodiagnosis of Hodgkin's disease in sputum specimens.

In patients with histologically proven Hodgkin's disease, knowledge of the extent of involvement of lymph nodes and other organs has proven valuable in the determination of treatment and prognosis. One of the most common sites of involvement outside the hematopoietic system has been shown to be the lung parenchyma; in this study, six patients with a tissue-proven diagnosis of Hogdkin's disease and positive cytologic findings in the sputum were reviewed. Three cell types not found in normal sputum specimens were identified in these patients and were correlated with the histologic patterns of the tumors as seen in lung biopsies. Our results suggest the usefulness of sputum examination as an adjunctive or possibly a substitute diagnostic procedure in the evaluation of patients with Hodgkin's disease and possible lung involvement. They also suggest that in some cases the cytologic diagnosis can be quite specific in the identification of neoplastic cells as consistent with a diagnosis of Hodgkin's disease.

Adult↗
PubMedDate not supplied

Diagnostic marker displays for intermediate cells from the uterine cervix.

Studies have shown the existence of computer-recognizable subvisual markers in intermediate cells from different cervical conditions that show good potential for discriminating between such "normal" cells from patients with normal, severe dysplasia/carcinoma in situ or invasive cancer cytologies. This paper presents examples of computer graphic displays, based on these markers, that could provide recognizable diagnostic clues to the cytologist, with a discussion of some of the factors involved in the interpretation of such displays.

Cervix Uteri↗
PubMedDate not supplied

Diagnostic marker features in dysplastic cells from the uterine cervix.

A study of dysplastic cells, visually classified as non-keratinizing (DYSN), keratinizing (DYSK) or severely dysplastic from an area of metaplasia (DYSM), demonstrated statistically significant differences between the same type of cell depending on the diagnostic category of the patient in which it originated: cases with moderate dysplasia (MOD), severe dysplasia/carcinoma in situ (CSD) or invasive carcinoma (INV). The results suggest that the cell types should include an indication of their origin, that, e.g., DYSN is an insufficient designation and that such cells should be denoted as DYSN-MOD, DYSN-CSD or DYSN-INV; this has major implications for the collection of cell image data bases for quantitative cytology as well as for the effort to characterize patients' cytologic samples by "cytodiagnostic profiles." The results also indicate that the samples as a whole contain many clues to patient diagnosis; examples are given of diagnostic profiles and the related computergraphic displays for different patient categories.

Carcinoma in Situ↗
PubMedDate not supplied

Potential of the in situ polymerase chain reaction in diagnostic cytology.

Routine examination of cytologic material by light microscopy in our laboratory includes special stains and enzyme histochemistry, but molecular biology techniques are not generally employed. We examined the feasibility of utilizing the in situ polymerase chain reaction (PCR) for examination of archival cytologic specimens. Protocols were shortened in an effort to employ a technique that would be economical and have diagnostic relevance; a result would be obtained within two days of a request. Cases of transitional cell carcinoma were examined for the p53 tumor suppressor oncogene; preparation of direct incorporation PCR required eight hours of laboratory work, thermal cycling was performed overnight, and product visualization required three hours of laboratory work the following day. Amplification products were found in the cytoplasm and nuclear regions with an antidigoxigenin fluorescent and peroxidase probe. In situ PCR has enormous potential in the diagnostic cytology laboratory.

Base Sequence↗
PubMedDate not supplied

Fine needle aspiration of mammary carcinoma with features of a carcinoid tumor. A case report with immunohistochemical and ultrastructural studies.

We report a case of fine needle aspiration (FNA) of a mammary carcinoma with features of a carcinoid tumor from the right breast of an 87-year-old woman who had a history of a left mammary "carcinoid" tumor diagnosed histologically three years earlier. Aspiration cytology revealed extremely rich cellularity. The neoplastic cells were mostly dispersed; however, they also could be found in loose clusters, acinus-like formations and occasionally in small sheets. They were relatively small and regular and contained fairly uniform, mostly round and oval nuclei with moderately abundant cytoplasm. Many of the tumor cells had eccentric nuclei. Immunohistochemical study revealed that tumor cells from both the cytologic smear and histologic section were reactive with antibody against serotonin. Ultrastructural study performed on the simple right mastectomy specimen showed many membrane-bound, dense-core particles in the cytoplasm of neoplastic cells, consistent with neuroendocrine secretory granules.

Aged↗