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Biomedical subjects

M Bergholz

Publications and source records attributed to M Bergholz.

At least 37 records · Page 2Linked to original sources
PubMed1989

Vaginal sonographic visualisation of a cervical carcinoma.

In the present case, the vaginal sonographic picture of a cervical (collum) carcinoma, which was clinically non-apparent, but was histologically established through a fractionated curettage, led to a change in the surgical procedure in the sense that a primary operation according to Wertheim--Meigs instead of a knife conisation was performed. The postoperative histology showed a good correlation between sonographic tumor boundaries and the actual expansion of the carcinoma.

Adult↗
PubMed1989

Regulation of tumor necrosis factor gene expression in colorectal adenocarcinoma: in vivo analysis by in situ hybridization.

Tumor necrosis factor (TNF) produced by macrophages is thought to contribute to the host defense against development of cancer. However, since tumor cells themselves are able to produce TNF, it is conceivable that TNF may also play an adverse pathological role in carcinogenesis. To better understand the functional significance of TNF in neoplastic disease, we have determined the cellular source of TNF activity produced in 10 patients with colorectal cancer. Northern blot analysis of RNAs extracted from fresh biopsy specimens revealed detectable TNF mRNA levels in all instances. By using in situ hybridization of frozen sections, scattered cells expressing TNF mRNA could be discerned. Based on morphological criteria, these TNF-positive cells most likely belong to the macrophage lineage. Macrophages in normal tissue surrounding the tumor did not express TNF mRNA, suggesting that macrophage activation occurs locally at the site of neoplastic transformation. Immunohistochemistry using anti-TNF monoclonal antibodies revealed that less than 1% of tumor-infiltrating macrophages synthesize TNF protein. Thus we present evidence that in colorectal cancer only a small proportion of tumor-infiltrating macrophages produces TNF, indicating that the microenvironment of the tumor provides adequate, yet suboptimal, conditions for macrophage activation.

Adenocarcinoma↗
PubMed1988

[Vaginal tumor--an initial manifestation of malignant histiocytosis].

An anaplastic vaginal tumour was diagnosed in a 29-year-old woman. Before the planned operation leukocytosis and thrombocytopenia developed and further diagnostic tests revealed malignant histiocytosis with bone-marrow infiltration, leukaemic wash-out and tumour formation in the lower pelvis. Intensive chemotherapy, as for acute myeloid leukaemia, achieved full remission. The case demonstrates that, although rare, tumorous organ infiltration may be the initial manifestation of malignant histiocytosis. Its early diagnosis and treatment decisively affects the prognosis.

Adult↗
PubMed1987

CFU-F circulating in cord blood.

CFU-F (colony forming units-fibroblast) were studied from cord blood and, as controls, from normal bone marrow of older children and adults. Numbers of CFU-F in cord blood buffy coat cells are lower by a factor of 10 in comparison to bone marrow CFU-F. Cytomorphology and staining with monoclonal antibody identify the progeny cells of CFU-F as fibroblasts. Cord blood CFU-F derived fibroblasts have properties supporting hematopoiesis: They produce CSF (colony stimulating factor) to which fresh cord blood CFU-GM (colony forming units-granulocytic, monocytic) react by colony formation in a dose-response manner. In addition, fibroblast colonies discharge clonogenic round cells into the medium forming CFU-GM and CFU-F colonies in secondary methyl cellulose cultures. We conclude that fetal blood contains clonogenic stromal cells (CFU-F) that give rise to fibroblasts with properties of hematopoietic support.

Adult↗
PubMed1986

[Immunohistochemical studies of the determination of the hormone receptor status of breast cancer].

Immunohistochemical examinations of 89 breast cancer specimens were performed using two different monoclonal antibodies. One marked the nuclear estrogen-receptor (ER) protein (anti-ER, Abbott), whereas the other marked an ER-related cytoplasmic protein (ER-D5 Amersham). Comparison of the results of the biochemical assay with those of the immunohistochemical markers revealed the following correlations: 75% anti ER and 71% ER-D5. Clinical follow-up studies are necessary to specify the relevance of these new immunohistochemical techniques in the anti-hormonal therapeutic management of breast cancer patients.

Antibodies, Monoclonal↗
PubMed1986

[Morphologic criteria in breast-saving surgery].

Efficiency of radiation therapy is dependent on the tumor mass on the one hand and on "radiosensitivity" on the other hand. Thus the locoregional acribic surgery is an urgently required presupposition regarding therapeutic success. In cases of early breast cancer (pT 1 N0 M0) unfavorable prognostic factors may initiate a discussion on adjuvant therapy. From the curative point of view at present breast preserving surgery referring tumors bigger that 2 cm (pT2) should be performed in cases with more advantageous prognostic parameters (low grade malignancy up to grade II a, low degree of tumor cell dissociation, favorable subtype, no relevant lymphangiosis carcinomatosa detectable, etc.). Additional immunohistochemical analysis of the receptor status and the proliferative compartment can be helpful for later therapeutic decisions. The possibility of breast preservation in only palliatively treated cases must be discussed separately.

Breast↗
PubMed1983

Overwhelming infection after splenectomy in spite of some spleen remaining and splenosis. A case report.

A fatal case of overwhelming postsplenectomy pneumococcal sepsis is presented occurring in a 37-year-old female 11 years after removal of the spleen because of traumatic rupture. The patient died 11 h after admission to hospital and about 32 h after sudden onset of illness. At necropsy splenic tissue, splenosis, disseminated intravascular coagulation, and thrombi within the arterioles consisting of gram-positive cocci and adrenal hemorrhage were found. The clinical, laboratory, and postmortem findings are described. Reports had been published of 41 other cases of overwhelming postsplenectomy infection (OPSI) in patients aged 20 years or more, but only three of these cases of OPSI syndrome occurred in spite of remaining splenic tissue. The longest interval between extirpation of spleen and subsequent sepsis was 42 years, indicating a small but lifelong risk of severe infection in asplenic patients. In view of the literature, the role of spleen in infection defence, the splenic function in blood clearance, and the prevention of postsplenectomy infections by antibiotic prophylaxis, pneumococcal vaccine, and reimplantation of autochthonous splenic tissue or infrared contact coagulation are discussed.

Adult↗
PubMed1981

[Granulomatous histiocytotic proliferation in the spleen of a two-year old girl (author's transl)].

After remittent fever for several weeks a two-year old girl developed a severe hypochromic microcytic anaemia, rapidly increasing splenomegaly and extensive leftsided pleural effusions. After splenectomy the girl recovered very fast. Elevation of erythrocyte sedimentation rate, sideropenia and microcytosis needed several months for normalisation. Histopathological examination of the enlarged spleen (weight 530 g) and regional lymph nodes revealed a marked granulomatous histiocytotic proliferation with erythrophagocytosis and siderosis. Considering morphological and clinical aspects the association of the reported case with histocytosis X seems to be justified.

Anemia, Hypochromic↗