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Biomedical subjects

M Beran

Publications and source records attributed to M Beran.

At least 217 records · Page 12Linked to original sources
PubMed1979

Myelopoietic enhancement by immunoadjuvants: in vitro studies for their rational use in neutropenic patients.

Following marrow transplantation, the clinical course of the patients is invariably complicated by severe infections due to neutropenia and immuno-incompetence. With a view to explore means of alleviating these complications, Bacillus Calmette-Guerin (BCG), methanol extraction residue of BCG (MER), Corynebacterium parvum (C. parvum) and Pyran were examined for their myelopoietic activity on human marrow. Light density (less than or equal to 1.077 g/ml) unfractionated (UF) cells (2 X 10(6)/ml and 2ml/dish), and adherent (Ad) and non-adherent (N-Ad) cells alone (derived from 4 X 10(6) UF cells per dish) were incubated with and without the above agents. The conditioned media (CM) were harvested at 24, 72, 96, and 168 hours and colony stimulating activity (CSA) assayed against light density non-adherent human marrow cells using double layer agar-culture system. CSA was maximum at 72 and 96 hours followed by an invariable decline at 168 hours' incubation. The optimal concentrations releasing maximum CSA varied for each agent. CM prepared with higher concentrations were less active. CM prepared in the presence of Pyran had no CSA. Comparisons of the maximally released CSA by the optimal concentrations of these agents revealed BCG and MER to be the most active. The CSA elaborated by UF cells was more than the total combined activity from separately incubated Ad and N-Ad cells. Re-addition studies revealed that almost full CSA could be recovered when Ad and N-Ad cells were incubated together at a ratio of 1:3. Different immunoadjuvants have a differing capacity to elaborate CSA from human marrow cells and each has an optimal concentration for maximum CSA release. This may require a cell-cell and/or humoral interaction(s) between Ad and N-Ad cells.

Adjuvants, Immunologic↗
PubMed1978

Liquid chromatographic determination of daunorubicin and daunorubicinol in plasma from leukemic patients.

A method is given for the determination of daunorubicin and its main metabolite, daunorubicinol, in plasma from leukemic patients after administration of daunorubicin as the free drug or as a complex with DNA. Daunorubicin and daunorubicinol are extracted from 2 ml of plasma (pH 8.1) using a mixture of chloroform and 1-heptanol (9:1). After re-extraction into phosphoric acid (0.1 M), the separation is performed as reversed phase liquid chromatography on a LiChrosorb RP-2 (5 micrometer) column with a mobile phase of acetonitrile-water, acidified with phosphoric acid. The precision, by quantitation with a photometric detector, was better than 2% within the range 20 ng/ml to 200 ng/ml. Some determinations of plasma levels of daunorubicin and daunorubicinol are presented.

Chromatography, Gas↗
PubMed1977

Preoperative facial paralysis in malignant parotid tumours.

Preoperative paralysis of the facial nerve was found in 145 of 1,029 patients with malignant parotid tumours (14%) treated at nine university clinics in Scandinavia. The incidence of facial paralysis varied between the different clinics. A parellelism between the incidence of the facial paralysis and the impairment of the prognosis of the different tumour types is shown. The presence of preoperative facial nerve paralysis in malignant parotid tumours implies a very poor prognosis but the situation is not as hopeless as has been suggested and therefore one must rely on very radical surgery.

Adenoma↗
PubMed1975

Serum levels of colony stimulating factor(s) and the growth of bone marrow cells in diffusion chambers.

Fluctuations in the body fluids of long-ranged humoral substance(s) capable of stimulating the growth of bone marrow granulocytic and macrophage-like cells in diffusion chamber cultures in vivo, was observed after whole body irradiation of mice. The fluctuation pattern was similar to that of the in vitro colony stimulating factor(s) of the sera of irradiated mice which indicates a relation between in vivo and in vitro active factor(s).

Animals↗
PubMed1975

The influence of mouse sera on colony formation and on the production of colony stimulating factor in vitro.

Results of studies on the in vitro production and release of colony stimulating factor (CSF), by bone associated cells cultivated in the presence or absence of mouse sera, are presented. Tissues from three strains of mice were shown to be equally effective in the production of CSF in vitro. Priming of the tissue donors by endotoxin 4 hours previously, or by irradiation 1, 7 or 15 days previously, significantly increased the CSF production. The addition of serum from normal mice to the culture medium had no effect on CSF production by tissue from normal mice but regularly inhibited CSF production by bones from irradiated animals. The inhibition was not due to the toxic effect of the sera on the CSF producing cells and was more pronounced in the cultures of NMRI than in the C57BL tissues. Some inhibition was also observed in cultures of tissue from endotoxin-primed NMRI mice. Extraction of the sera with ether removed the inhibitory material. The sera which inhibited the CSF production in vitro also inhibited the growth of granulocytic and macrophage colonies in soft agar cultures but did not inhibit the growth and DNA synthesis of cultured L-cells.

Animals↗