Search PubMed⌕ Search

Biomedical subjects

M Beaudoin

Publications and source records attributed to M Beaudoin.

At least 19 recordsLinked to original sources
PubMed1997

Hemodynamic ischemic stroke during carotid endarterectomy: an appraisal of risk and cerebral protection.

PURPOSE: The purpose of this study was to validate the commonly accepted indicators of risk of ischemic stroke that indicate the necessity for cerebral protection during carotid endarterectomy (CEA), and to examine the efficacy of high-dose thiopentone sodium (thiopental) as a cerebral protection method in patients who are at high risk of intraoperative ischemic stroke. METHOD: In a prospective study of 37 CEAs performed for symptomatic stenosis > 70%, functional and clinical indicators of risk of ischemic stroke during carotid cross-clamping were identified. Functional indicators of risk were the development of ischemic electro-encephalogram (EEG) changes and stump pressure < 25 mm Hg. Clinical indicators of risk were previous ischemic hemispheric stroke and severe bilateral disease. These indicators were correlated in all patients, some of whom had two or three coexisting indicators of risk. The EEG and stump pressure were monitored continuously during carotid occlusion in all operations. Carotid occlusion times were recorded. Intraluminal shunting was eliminated in favor of high-dose thiopental cerebral protection in all patients. Neurologic outcome was deemed to measure the efficacy of thiopental protection in patients who are identified to be at risk and, hence, in need of cerebral protection. The validity of the indicators used to identify risk of ischemic stroke during CEA was assessed. RESULTS: The absolute stroke risk was found to be 29.7% for the whole group (37 patients) and 57.9% in 19 patients who had commonly accepted indications for protective shunting. The correlation of ischemic EEG changes with stump pressure < 25 mm Hg was only 27.3%, whereas the expected correlation based on well-documented reports in the literature was 100%. The lack of correlation may have been related to the prevention of ischemic EEG changes by thiopental. There were no neurologic deficits in the series. CONCLUSIONS: The absence of neurologic deficit in the study indicated that thiopental protection was effective in preventing ischemic stroke in high-risk patients and safely replaced intraluminal shunting.

Aged↗
PubMed1997

Evaluation of Interventions for Prevention of Back, Neck, and Shoulder Disorders in Three Occupational Groups.

An epidemiologic study was carried out in order to evaluate the effects of prevention programs at the workplace aimed at reducing back, neck, and shoulder morbidity among active workers. The intervention group included 275 workers in three occupational subgroups: hospital workers, warehouse workers, and office workers. The control group included 250 workers as comparable as possible to the intervention group. Comparisons were made, according to one-year changes in morbidity scores, for low back, upper back, neck, and shoulder disorders separately. An overall measure was also used. The one-year change in the overall measure was significantly different between the intervention group and the control group, indicating a positive effect of the prevention programs. Positive effects were stronger for some sites of pain and some occupational groups.

Journal Article↗
PubMed1996

Pseudoaneurysm of the proper hepatic artery with duodenal fistula appearing as a late complication of blunt abdominal trauma.

Posttraumatic pseudoaneurysms of the hepatic artery are rare and usually occur as a complication of open abdominal trauma. Even less common is the coexisting presence of enteric fistulization. We report a patient with upper gastrointestinal hemorrhage occurring 3 years after blunt abdominal trauma resulting from a pseudoaneurysm of the proper hepatic artery with duodenal fistulization. The patient was treated successfully by ligation of the proper hepatic artery and closure of the duodenal opening.

Abdominal Injuries↗
PubMed1992

Studies on a murine model for evaluation of virulence of Streptococcus suis capsular type 2 isolates.

Five different parameters, time of incubation of the culture, type of culture medium, inoculum, strain of inbred mice, and age of mice, were tested using the LD50 technique to standardize a murine model for the evaluation of the virulence of Streptococcus suis capsular type 2 isolates. A model using 28 day-old mice belonging to CF1 strain appeared to give the best results. The inoculum size was the parameter most influencing the 50% lethal dose obtained with mice. Inoculation with 1-ml volume of a bacterial suspension instead of 0.1 or 0.5 ml decreased the LD50. The standardized model was used to evaluate the virulence of some isolates of known pathogenicity for pigs. The minimum lethal dose was used in the model and it appeared that the virulence of Streptococcus suis capsular type 2 isolates can be measured from highly virulent to totally avirulent.

Animals↗
PubMed1992

Effect of neonatal degranulation on the morphological development of rat CA3 pyramidal neurons: inductive role of mossy fibers on the formation of thorny excrescences.

Intracellular peroxidase injections were made on CA3 pyramidal neurons from slices obtained from 3-60 day old control and experimental rats. Experimental rats had been irradiated at birth with gamma-rays to destroy the granule cells of the fascia dentata. Neonatal gamma-ray irradiation induced a clear cut reduction of the total number of granule cells (80% decrease at P60) and mossy fibers as revealed by Nissl and Timm stains. The number of branching points and segments, total dendritic length, and density of dendritic spines in the stratum radiatum and stratum oriens on CA3 pyramidal cells were not significantly different between control and irradiated rats. To quantify the development of thorny excrescences we measured the number and total area of the thorny excrescences per neuron at different stages of development. gamma-Ray irradiation induced a reduction in the number and total area of thorny excrescences. This effect was readily apparent by postnatal day (P) 12, and a reduction of 70% in the total area was observed at P30. In conclusion, gamma-ray irradiation destroys the majority of granule cells and induces a reduction in the development of thorny excrescences. Our data strongly suggest that this effect is directly caused by the lack of mossy fibers rather than by the irradiation itself since other parameters (i.e., the number of segments and branching points, density of non-mossy spines, and total dendritic length) were not affected. Therefore, we suggest that mossy fibers play an inductive role in the formation of thorny excrescences.

Aging↗
PubMed1992

Molecular analysis of isolates of Streptococcus suis capsular type 2 by restriction-endonuclease-digested DNA separated on SDS-PAGE and by hybridization with an rDNA probe.

This study was undertaken to assess the discriminatory value of restriction endonuclease (RE) digestion patterns of Streptococcus suis chromosomal DNA using polyacrylamide gel electrophoresis (SDS-PAGE) and DNA-rDNA hybridization. For the RE digestion patterns, DNAs were digested separately with the enzymes BamHI and BglII and the resultant fragments were separated by SDS-PAGE. An Escherichia coli rDNA probe derived from pKK3535 was used for the hybridization. Twenty-three S. suis capsular type 2 isolates recovered from diseased and clinically healthy pigs, from a human case, and from a cow were compared in this study. The majority of isolates associated with septicaemia belonged to one restriction endonuclease analysis (REA) profile group. Isolates associated with pneumonia belonged either to the REA profile group of isolates associated with septicaemia or to a second REA profile group. The REA profiles of isolates from clinically healthy animals were more heterogeneous. The REA profile of the type 2 reference strain, S735, which was originally isolated from a pig, was very different from those of the porcine and bovine isolates but similar to the profile of the human isolate. The profiles obtained after rDNA hybridization were more homogeneous. Although different patterns were detected in the 23 isolates, there was no correlation between the source of the isolate and the patterns observed with this technique.

Animals↗
PubMed1991

Computer analysis and pattern recognition of Doppler blood flow spectra for disease classification in the lower limb arteries.

In the present study, a computer processing method was developed to objectively classify disease in the lower limb arteries evaluated by noninvasive ultrasonic duplex scanning. This method analyzes Doppler blood flow signals, extracts diagnostic features from Doppler spectrograms and classifies the severity of the disease into three categories of diameter reduction (0-19%, 20-49% and 50-99%). The features investigated were based on frequency features obtained at peak systole, spectral broadening indices and normalized amplitudes of the power spectrogram computed in various positive and negative frequency bands. A total of 379 arterial segments studied from the aorta to the popliteal artery were classified using a pattern recognition method based on the Bayes model. Two classification schemes using a two-node decision rule were tested. Both schemes gave similar results, the first one provided an overall accuracy of 83% (Kappa = 0.42) and the second an overall accuracy of 81% (Kappa = 0.35) when compared with conventional biplane contrast arteriography. These performances, especially for the 0 to 19% lesion category, are better than the one obtained by the technologist (accuracy = 76% and Kappa = 0.33), based on visual interpretation of the Doppler spectrograms. To recognize hemodynamically significant stenoses (50-99% lesions), the pattern recognition system has a sensitivity and a specificity of 50% and 99%, respectively, using classification scheme I. With classification scheme II, the sensitivity and the specificity are 50% and 98%, respectively. Visual interpretation of the Doppler spectrograms leads to a sensitivity and a specificity of 50% and 98%, respectively. These results are the first to be obtained by a pattern recognition system in classifying lower limb arterial stenoses.

Adult↗
PubMed1991

Effects of neonatal gamma-ray irradiation on rat hippocampus--I. Postnatal maturation of hippocampal cells.

The axons of dentate granule cells, the mossy fibres, establish synaptic contacts with the thorny excrescences of the apical dendrite of CA3 pyramidal neurons. Dentate granule granule cells develop postnatally in rats, whereas the CA3 pyramidal cells are generated before birth. In the present studies, using unilateral neonatal gamma-ray irradiation to destroy the granule cells in one hemisphere, we have studied the effect of mossy fibre deprivation on the development of their targets. We show that such "degranulation" prevents the normal development of giant thorny excrescences, suggesting that the development of thorny excrescences in CA3 pyramidal neurons is under the control of mossy fibres. In contrast, irradiation of the hippocampus of the neonatal rat does not affect the development of the dendritic arborization of CA3 pyramidal cells and their non-mossy dendritic spines.

Animals↗
PubMed1991

Characterization of six new capsular types (23 through 28) of Streptococcus suis.

Six new capsular types of Streptococcus suis (types 23 to 28) are described. All reference strains were isolated from diseased pigs and were morphologically and biochemically similar to previously described capsular types 1 to 22. Clear and specific reactions were obtained for each of the new capsular types with three different typing techniques; no cross-reactions were detected among them or with other S. suis capsular types. Their capsular material presented similar ultrastructural characteristics, as shown by electron microscopy, and fimbriae similar to those described for other capsular types of S. suis were observed. When untypeable field isolates were tested with antisera raised against the six new capsular types, capsular type 23 appeared to be the most prevalent, representing more than 50% of all these isolates. Most isolates were recovered from cases of pneumonia, septicemia, and meningitis. Presumptive biochemical identification described for S. suis capsular types 1 to 22 may also be used for capsular types 23 to 28.

Animals↗
PubMed1991

Isolation and characterization of Streptococcus suis capsular types 9-22.

The incidence and biochemical patterns of Streptococcus suis capsular types 9-22 are presented. Of 148 untypeable (with types 1-8 antisera) isolates of S. suis recovered from diseased pigs, 10% were not capsulated. Of the remaining 134 isolates, only 53% belonged to capsular types 9-22; capsular types 22 and 9 were the most prevalent, representing 19% and 13%, respectively. Capsular type 15 (de Moor's group T Streptococcus) is reported here for the first time in North America since it was described in 1963 in Europe. Of 188 untypeable isolates recovered from clinically healthy pigs, 25% were noncapsulated. Of the remaining 141 isolates, 90% belonged to the new capsular types, and 87% were identified as 1 of 4 types: 17, 18, 19, and 21. Capsular types 12 and 20 were not detected among the Canadian isolates. Almost half of strains were arginine dihydrolase-negative, and 45% fermented mannitol, which is seldom a positive test with capsular types 1-8. Although some strains were negative with salicin or trehalose, none were negative for both sugars. Only 54% of isolates tested with 1 rapid multitest system were correctly identified as S. suis. A tentative biochemical profile that might be used with a microplate identification system is also presented. Biochemical identification using the conventional system instead of the rapid multitest system is preferable.

Agglutination Tests↗
PubMed1990

Effects of kainic acid-induced seizures and ischemia on c-fos-like proteins in rat brain.

We have analyzed the brain pattern and time-course of c-fos-like proteins expression in kainic acid-induced seizures in the rat. C-fos-like immunoreactivity increased initially in the hippocampus, notably in the dentate gyrus, at the time of the first limbic motor seizure (90 min after kainate). C-fos-like labelling progressively involved different structures of the limbic system when the rats manifested a permanent epileptic state (3-6 h). The labelling was still conspicuous 12 h after kainate treatment and progressively declined to reach control levels 48 h after kainate. This time-course is similar to that produced by kainic acid on 2-deoxyglucose consumption and correlates with the electrographic changes previously described, supporting the idea that c-fos-like immunostaining may provide a useful marker of neuronal activity, with a cellular resolution. Since anoxic-ischemic treatment produces a very slight and transient increase in c-fos-like immunostaining restricted to the fascia dentata, c-fos-like expression is seizure-related and not due to a local hypoxia or ischemia.

Amino Acid Sequence↗
PubMed1990

Does the radial arterial line degrade the performance of a pulse oximeter?

The presence of radial arterial lines may diminish distal digital perfusion. Using a pair of pulse oximeters on the index fingers of cannulated and noncannulated arms the saturation and oximeter pulse strength were assessed. Three hundred paired measurements in fifty consecutive Intensive Care patients were undertaken more than two hours after cannula insertion. The pulse strength was 19.7 (SD 6.1) and 19.7 (SD 5.7) on the cannulated and noncannulated sides while the saturation was 97.3 (SD 2.3) and 97.1 (SD 2.4) respectively. These differences are not clinically important and the study demonstrates that reliable pulse oximetry measurements may be made distal to a radial artery cannula. It is recommended that in each individual the placement of the sensor be such as to ensure the best signal and the most stable saturation readings.

Catheterization, Peripheral↗
PubMed1990

Streptococcus suis infection in swine. A sixteen month study.

A total of 349 isolates of Streptococcus suis retrieved from different tissues from diseased pigs were examined in this study. Only 48% of them could be categorized as one of serotypes 1 to 8 and 1/2. Among typable isolates, serotype 2 was the most prevalent (23%), followed by serotype 3 (10%). The majority of all isolates originated from lungs, meninges/brain, and multiple tissues. Forty-one percent of typable isolates and 33% of untypable isolates were retrieved in pure culture. Other isolates were found in conjunction with Pasteurella multocida, Escherichia coli, Actinobacillus pleuropneumoniae, Actinomyces pyogenes, and other streptococci. Typable S. suis isolates were more frequently isolated from pigs between five and ten weeks of age, while untypable isolates were mostly found in animals aged more than 24 weeks. No obvious monthly and/or seasonal variation of the prevalence of isolation of S. suis could be detected.

Animals↗