[A HeLa subline with a genetic line count of 59 chromosomes].
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Animals↗
Biomedical subjects
M Bauchinger
Publications and source records attributed to M Bauchinger.
Comparative genomic hybridisation for the analysis of chromosomal imbalances in solid tumours and haematological malignancies.
Comparative genomic hybridisation (CGH) is based on a two-colour, competitive fluorescence in situ hybridisation of differentially labelled tumour and reference DNA to normal metaphase chromosomes. This new technology has made a great impact in molecular tumour pathology due to its possible application to archival specimens and the ability to create copy number karyotypes throughout the whole genome from very small amounts of DNA. If chromosomal imbalances can be correlated with a etiological and clinical features of tumours, CGH could be able to provide new prognostic and diagnostic criteria. CGH findings further provide starting points for the molecular genetic characterisation of altered chromosomal regions harbouring yet unidentified genes involved in tumorigenesis and tumour progression. An overview of the results of published CGH studies on solid tumours and haematological malignancies is presented. Methodological limitations of the CGH technology are reported, as well as future developments which will improve its use in routine analysis.
Chromosome Aberrations↗
[Time relationships of radiation-induced chromosome aberrations in humans].
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Adult↗
Chromosome changes in lymphocytes after occupational exposure to pentachlorophenol (PCP).
Chromosome analyses were carried out on peripheral lymphocytes from 22 male workers employed at a pentachlorophenol (PCP) producing factory. As compared with a group of 22 matched controls a small, but significant, increase in the frequency of dicentrics and acentrics was observed. There was no significant increase of sister-chromatid exchange (SCEs) in smoking PCP workers, as compared with smoking controls. Within the control group, smokers had a higher incidence of SCEs than non-smokers.
Air Pollutants↗
Fecapentaene causes sister-chromatid exchanges in human lymphocytes.
Fecapentaenes are potent mutagenic compounds found in human feces that are considered as potential colon carcinogens. It is demonstrated that a synthetic racemic all-trans fecapentaene-12 (fec-12) causes a strong dose-dependent increase in the frequency of sister-chromatid exchanges (SCE) in human lymphocytes exposed at different stages of the cell cycle. The SCE-inducing capacity is consistent with published results on the DNA-damaging activity of fec-12 such as formation of DNA single-strand breaks and interstrand cross-links.
Cell Cycle↗
Chromosome changes with time in lymphocytes after occupational exposure to toluene.
Chromosome analyses were carried out in peripheral lymphocytes of 27 workers exposed to toluene in a rotogravure plant. At the time of blood sampling all of them had not been exposed to toluene for at least 4 months up to 5 years. Up to 2 years after cessation of exposure to toluene a higher incidence of chromatid-type aberrations could be observed than in controls. After longer post-exposure periods the aberration yields can no longer be distinguished from background level. No differences were revealed in SCE frequencies of smoking or non-smoking workers post toluene exposure compared with the corresponding controls.
Chromosome Aberrations↗