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Biomedical subjects

M Bastide

Publications and source records attributed to M Bastide.

At least 55 records · Page 3Linked to original sources
PubMed1980

[Evaluation of total serum complement by radial immunohemolysis in allergic asthma].

The role of the complement system in human allergic asthma has been studied by serum complement evaluation by radial immuno-hemolysis. Various groups were tested: 50 normal subjects, 49 allergic patients, 6 pollen sensible patients during and after pollinic season. Complement was evaluated during and after allergen challenge (11 patients). No significative variations were established neither during the challenge nor after (15 mn, 45 mn, 1 h).

Asthma↗
PubMed1979

Taxonomic significance of yeast sphaeroplast release after enzymatic treatment of intact cells.

Treatment of whole yeast cells with a mixture of a reducing agent and 1,3-beta-glucanase isolated from Basidiomycete QM806 led to the production of sphaeroplasts from ascomycetes, from some fungi imperfecti, but not from basidiomycetes. Association of 1,3-beta-glucanase with a second enzyme, 1,4-alpha-glucanase, from Trichoderma viride, was required for sphaeroplast release from some, but not all, basidiomycetes and fungi imperfecti. The ability of yeast cells to liberate sphaeroplasts following appropriate enzymic treatment is proposed as a taxonomic criterion for differentiating basidiomycetous from ascomycetous yeasts and for classifying fungi imperfecti yeasts.

Ascomycota↗
PubMed1976

[Action of various beta(1-3)-D-glucanases on the wall of yeasts: taxonomic applications].

Purified beta-(1-3)-D-glucanases (from Aspergillus nidulans, Badidiomycetes sp. QM 806, Trichoderma viride) are used to release protoplasts from various yeasts. Two of them may prove taxonomic correlation. The enzyme of Basidomycetes sp. releases protoplasts from Ascomycetes, the enzyme of T. viride releases protoplasts from Ascomycetes and Heterobasidiomycetes; none of them acts on the Basidiomycetes.

Ascomycota↗
PubMed1976

[Remarks on the modifications of cell wall during the sporulation of Saccharomyces cerevisiae Hansen (author's transl)].

Evolution of cell wall during sporulation was studied by means of scanning electron microscopy and by immunological techniques. Experiments were done simultaneously with a strain a/alpha able to sporulate and a strain alpha/alpha unable to sporulate. Under such conditions it was possible to clarify whether the changes observed were related to the sporulation or to the culture conditions. Cell wall structure modifications during sporulation were not obvious morphologically but have been revealed by immunological methods. During vegetative growth, antigenic sites of strains a/alpha and alpha/alpha were different. During incubation in the sporulation medium, antigenic structure of the cell wall was modified. Some antigenic sites seem to be specific of sporulation.

Cell Wall↗
PubMed1975

[The enzymatic hydrolysis of cell wall applied to yeasts classification (author's transl)].

The rigid structure of yeast cell wall may be a taxonomic criterion. Using the Helix pomatia juice (with mercaptoethylamine hydrochloride) as a polyenzymatic system, we have always obtained protoplasts from ascomycetous yeasts and never from basidiomycetous yeasts tested. Then, we have applied this enzymatic analysis to the Fungi imperfecti yeasts and, according to this test, separated genus looking like basidiomycetous behaviour: Trichosporon, Pityrosporum, Rhodotorula, Cryptococcus and non fermentative Candida (group II). Oppositely, we have put together the Fungi imperfecti yeasts looking like ascomycetous behaviour: Kloeckera, Trigonopsis, Brettanomyces and fermentative Candida (group I). With the second lytic system made of mercaptoethylamine-HCl and beta-(1-3)-D-glucanase, we have selected ascomycetous yeasts giving protoplasts: Saccharomycoidea with genus Saccharomyces, Kluyveromyces, Pichia, Hansenula, Citeromyces, Debaryomyces; we have joined Saccharomycodes and Hanseniaspora to this second group. The other ascomycetous yeasts studied did not give protoplasts with the definite lytic system made of purified beta-(1-3)-D-glucanase and thiol. These results seem to demonstrate that the structure of cell wall can act as an important taxonomic criterion.

Ascomycota↗
PubMedDate not supplied

[Pharmacokinetic study of a new benzyl-1 isoquinoline derivative (458 L) after oral administration in men].

Pharmacokinetic study of 458 L. via oral route, was performed in twelve normal volunteers, in a randomized crossover design of three galenic forms. Plasma concentrations were determined, after extraction, by HPLC, with spectrofluorimetric detection. Plasmatic kinetics were fitted to a bi-exponential function with a distribution half-life of 1h and an elimination half-life of 11.5 to 14.7 h according to the form. Pharmacokinetic parameters evaluated as area under the curve (AUC), maximum plasma concentrations (Cmax), relative bioavailability (F'), have shown strong interindividual differences, specially in women group, and this with tobacco smoking.

Administration, Oral↗