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Biomedical subjects

M Bard

Publications and source records attributed to M Bard.

137 records · Page 8Linked to original sources
PubMedDate not supplied

Membrane fluidity alterations in a cytochrome P-450-deficient mutant of Candida albicans.

A cytochrome P450-deficient mutant of the pathogenic fungus, Candida albicans, which accumulates exclusively 14 alpha-methylsterols in place of the normal end product sterol, ergosterol, was examined for alterations in membrane fluidity by electron paramagnetic resonance. The results using four nitroxyl spin labels indicated that exponential phase cultures of the mutant strain, D10, had a uniformly more rigid membrane than similarly grown wild type. Since D10 shows a sterol spectrum similar to that of wild type cells treated with imidazole and triazole antifungal agents, many of the physiological effects reported as the result of azole application may be the result of alterations in membrane fluidity.

Animals↗
PubMedDate not supplied

Polyene resistance in ergosterol producing strains of Candida albicans.

Following nitrous acid mutagenesis, one nystatin- (nyl) and two amphotericin B (AB)-resistant (ab1 and ab2) mutants of Candida albicans were isolated and characterized. The three mutants plus a previously described cytochrome P450-deficient mutant (D10) of this organism were analyzed for polyene cross resistance. Cross resistance was noted for ny1 and D10 but not for ab1 and ab2. Sterol analysis indicated that ny1 was a delta 8-delta 7 isomerase mutant while ab1 and ab2 showed wild type sterol profiles. Fatty acid analysis showed no significant differences for ab1, ab2, and ny1 compared to wild type while D10 showed more pronounced differences. AB- and Triton X-100-induced potassium leakage studies indicated that ab1 and ab2 are resistant to low AB levels and ny1 is resistant to higher AB levels. In contrast, ab1 and ab2 were more resistant to detergent-induced potassium leakage than the wild type or mutants ny1 and D10. Significant differences in growth rate, ethanol sensitivity, and response to Tergitol were also noted among the resistant strains. The data indicate a different mechanism of action for the two polyenes and indicate a resistance mechanism for ab1 and ab2 based on subtle alterations of membrane structure rather than sterol substitution.

Amphotericin B↗
PubMedDate not supplied

Physician executives report high job satisfaction. Summary of findings from a survey of senior physician executives.

The role of the senior physician executive is well established in American hospitals and health systems. There is little research, however, on overall physician executive job satisfaction, their perceptions of their organizational role and job performance, or their views of the medical staffs with which they work. A recent survey of physician executives examined these and other areas. It found physician executives to be quite satisfied with their jobs. What follows is a summary of the findings. An article based on the survey will be featured in a future issue of The Physician Executive.

Data Collection↗
PubMedDate not supplied

[Synovial sarcoma].

Explore the source record for details and available documents.

Adolescent↗
PubMedDate not supplied

[Radio-anatomical guides for the dorsal puncture-biopsy].

This paper describes a specific prone oblique view very helpful to thoracic vertebral bone biopsy under fluoroscopic control. In this position the costal head and neck axis gives the direction of the procedure. A puncture between two ribs following this direction easily avoids both the pleura and spinal canal. This thoracic biopsy of the vertebral body and disc under fluroscopic control becomes a reliable diagnostic tool.

Biopsy, Needle↗