Search PubMed⌕ Search

Biomedical subjects

M Baquero

Publications and source records attributed to M Baquero.

At least 37 records · Page 2Linked to original sources
PubMed1996

[Diagnosis of 4 cases of intestinal microsporidiosis in AIDS patients].

BACKGROUND: Most of the latest clinical and epidemiologic studies indicate that microsporidiosis and above all Enterocytozoon bieneusi cause approximately 7-50% of otherwise unexplained diarrhea in HIV-infected patients. Four cases of intestinal microsporidiosis in AIDS-patients are reported. METHODS: Detection of spores of microsporidiosis in stool samples from HIV-infected patients was performed by the following techniques: 1) light microscopy, using Kokoskin's modification of the Weber trichrome stain; 2) electron microscopy, for confirmation (only available in one case), and 3) indirect immunofluorescence assay (IIF), performed on all positive samples. Rabbit polyclonal antibodies anti-Encephalitozoon cuniculi, anti-E. hellem and anti-E. (Septata) intestinalis were used to eliminate the possibilities of presence of microsporidiosis other than E. bieneusi. RESULTS: Detection of spores of microsporidiosis by trichrome stain was positive in all samples from the 4 patients. By elimination, IIF confirmed the presence of Enterocytozoon bieneusi in all 4 cases. E. bieneusi was identified by electron microscopy in the only case available. Three patients had CD4+ lymphocyte counts < 100 x 10(6)/l and one < 500 x 10(6)/l. All had AIDS and three of them were homosexual. Two patients remained positive for microsporidiosis for almost 5 months. CONCLUSIONS: Despite the very few cases described in Spain, these preliminary data lead us to believe that intestinal microsporidiosis should be considered as an possible cause of diarrhea in our country also, and mainly in acute immunodeficiency patients.

Acquired Immunodeficiency Syndrome↗
PubMed1995

[Celiac disease with occipital calcifications: 2 late cases].

The association of celiac disease, epilepsy and occipital calcifications with initial clinical manifestations of epilepsy during the first two decades of life with an often progressive and variable course and clinical expression of malabsorption has recently been described. Two cases of celiac disease with occipital calcifications and a presentation with neurologic symptoms in adulthood are reported. The first case is that of a 40-year-old male who presented recurrent and alternating pure brachial monoparesis and later acute abdominal pain following which celiac sprue was diagnosed. The second case is that of a 53-year-old woman diagnosed with celiac sprue 20 years before, presenting permanent myoclonus in the lower limbs which were progressive in severity, ataxic march and generalized tonoclonic seizures. Both patients had bilateral occipital calcifications on CT and celiac disease was demonstrated on biopsy. The first case also showed marked signal alteration in the white matter on MRI. Celiac disease with cerebral calcifications presents also in adulthood with atypical clinical manifestations. Suspicion of celiac disease may be confirmed by non-invasive methods such as antigliadin and antiendomysium antibody determination. CT imaging is characteristic.

Adult↗
PubMed1994

Progressive bleeding in spontaneous thalamic hemorrhage.

Progressive bleeding is uncommon in spontaneous intracerebral hemorrhage. We describe two cases of spontaneous thalamic hemorrhage with evidence of active bleeding or rebleeding detected by noncontrast CT. The first patient was a 47-year-old man with a history of hypertension and cocaine abuse. The second patient, a 73-year-old man, had no valuable risk factors. It is noteworthy that most well-documented instances of continued bleeding or rebleeding in spontaneous intracerebral hemorrhage occur in the thalamus. The natural evolution of spontaneous intracerebral hemorrhage after the first hours of stroke remains to be elucidated.

Aged↗
PubMed1992

[Evidence of infection by HIV-2 in Madrid (1991)].

The presence of antibodies against HIV-2 was analyzed in serum samples from high-risk individuals for retrovirus infection, who were seropositives for HIV-1. Out of a total of 842 samples, 344 presented positive absorbency and 16, threshold ranges, in an enzymatic immunoassay (EIA) designed to detect simultaneously antibodies against HIV-1 and HIV-2. The Western Blot (WB) analysis confirmed the presence of antibodies against HIV-1 in 324 samples with positive or threshold EIA, while it was indetermined in 15 samples. WB was negative in 11 samples. In the 15 samples with indetermined WB and in the 16 samples with threshold EIA, the presence of antibodies against HIV-2 was investigated with an specific WB and a test designed with synthetic peptides (Pepti-lav). Three samples met the isolated seropositivity criteria for HIV-2. Those samples were from 3 african immigrants living in Madrid, which, until now, were asymptomatic, although one of them presented biological signs of immunodeficiency. None of them referred high-risk infectious practices, such a homosexuality or drug-addiction, although all of them referred heterosexual contacts in their native countries. Samples from native subjects meeting serum positivity criteria for HIV-2 were not identified.

AIDS Serodiagnosis↗
PubMed1990

[Lymphocyte subpopulations in the evolution of brucellosis].

We have sequentially studied for one year CD3+, CD4+, CD8+, CD11b+, and CD20+ lymphocyte subpopulations in 52 brucellosis patients whose diagnosis was confirmed by hemoculture. We observe at diagnosis a decreased percentage of CD4+ lymphocytes with an increase in CD8+ and inverted CD4+/CD8+ ratio, which was greater in patients with an evolution longer than 4 weeks (p less than 0.05) returning to normal very slowly. The percentage of monocytes was also increase at diagnosis (p less than 0.01) and at their second month CD3+ an CD20+ subpopulations did not suffer any changes during the follow up period. Patients were divided into two groups according to whether one or two anti-Brucella IgG peaks were observed in ELISA throughout the evolution. Patients in whom IgG titer decreased uninterruptedly, the inversion of the ratio during the first two months was greater, while those patients who suffered a relapse showed a simultaneous new decrease in CD4/CD8 ratio (p less than 0.05).

Antibodies, Bacterial↗
PubMed1989

Changes in IgM and IgG antibody concentrations in brucellosis over time: importance for diagnosis and follow-up.

Changes in concentrations of IgM and IgG antibodies to Brucella were monitored for at least 13 mo by an enzyme-linked immunosorbent assay (ELISA) in 52 patients with culture-positive brucellosis. Two main patterns were observed. After an initial peak, 29 patients (56%) had a steady drop in their IgG levels, whereas 23 (44%) had more than one peak over time. All patients with a chronic form of brucellosis or a relapse were in the second group. In most cases, Brucella antibodies, although falling to low levels, remained measurable. Cutoff levels for IgM and IgG were calculated after considering serum antibody concentrations in people who had recovered from an infection. A separate normal range was established for occupationally exposed workers. On admission, sera from all patients contained Brucella antibody levels greater than established cutoff levels. Our results show that ELISA is an excellent method for diagnosis and follow-up of brucellosis.

Brucellosis↗
PubMed1989

[Bacteremia caused by Salmonella non-typhi in patients infected with HIV].

All the cases of HIV infected patients diagnosed in our department during 1987 of Salmonella bacteremia are included in this report. Out of a total of 10 patients with both conditions, six were diagnosed of AIDS, three of AIDS related complex, and one presented an asymptomatic HIV infection. The mean age was 32.9 years (range 22 to 56). Eight of them were males, seven were intravenous drug addicts, and three were homosexual. Fever was observed in all cases and diarrhea in four of them. Salmonella enteritidis was isolated from the blood culture of nine patients and salmonella typhimurium from one. Salmonella enteritidis was isolated from the stool culture of 5 patients and Salmonella typhimurium and Campylobacter from the stool culture of one patient. Of the 10 patients 6 have died, 4 of them within the three weeks after the diagnosis of Salmonella non-typhi bacteremia, 2 have not relapsed and two patients were lost during follow up.

Acquired Immunodeficiency Syndrome↗