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Biomedical subjects

M Bader

Publications and source records attributed to M Bader.

At least 145 records · Page 8Linked to original sources
PubMed1990

Authoritarianism and attitudes toward AIDS victims.

This study investigated the relationship of authoritarianism and attitudes toward AIDS victims in three samples. One hundred fifty-eight students at Oregon State University participated, including 101 students from the United States, 25 from Japan, and 32 from other Asian societies. The survey instrument included the 20-item Attitudes Toward AIDS Victims (ATAV) Scale, the 18-item F Scale, the Form A, and 14-item Formal Content of Dogmatism Scale. Results showed slight but significant correlations between the ATAV and F (r = .17, p less than .044) and Formal Content of Dogmatism (r = .20, p less than .023) Scales for the United States sample. Highly significant differences were found in the predicted direction among the three samples on authoritarianism, F = 43.94, p less than .001.

Acquired Immunodeficiency Syndrome↗
PubMed1989

Characterization of an inhibitor of protein synthesis initiation from mouse erythroleukemia cells.

This study describes the partial purification of a translational inhibitor from mouse erythroleukemia (MEL) cells. It is present in MEL cells induced to erythroid differentiation and in uninduced cells in approximately equal amounts. The inhibitor blocks initiation but not elongation of in vitro protein synthesis in the rabbit reticulocyte lysate and in extracts prepared from induced or uninduced MEL cells. Nuclease-resistance, heat-sensitivity and the chromatographic behaviour of the inhibitor indicate that it is a protein with a relative molecular mass of approx. (45-70).10(3). The inhibitor has no eIF-2 alpha phosphorylating activity and does not affect the formation of the ternary complex [eIF-2.GTP.Met-tRNAf] nor the binding of Met-tRNAf to the 40 S ribosomal subunit. The inhibitor interferes with the binding of mRNA to the 43 S preinitiation complex, independent of the presence of the m7GTP cap of the mRNA.

Animals↗
PubMed1989

Binding of mRNA by an oligopeptide containing an evolutionarily conserved sequence from RNA binding proteins.

Several proteins with an affinity to RNA contain a conserved sequence of 8 amino acids which is postulated as being important for RNA binding. An oligopeptide of 11 amino acids containing this sequence is shown to bind 32P-labelled globin mRNA in a filter binding assay. High concentrations of heparin compete for this binding. 10 other peptides with different sequences do not exhibit affinity to RNA in this assay. These results support the relevance of the conserved peptide sequence in the binding of proteins to RNA.

Amino Acid Sequence↗
PubMed1989

Differential effect of hemin-controlled eIF-2 alpha kinases from mouse erythroleukemia cells on protein synthesis.

Cultured mouse erythroleukemia (MEL) cells can be induced to erythroid differentiation by a variety of chemical agents. This differentiation process is marked by the onset of globin mRNA and hemoglobin synthesis. In rabbit reticulocytes, globin synthesis is regulated by a hemin-controlled translational inhibitor (HCI) which acts via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (eIF-2). From both uninduced and induced MEL cells, hemin-controlled eIF-2 alpha kinases have been partially purified. They resemble HCI with respect to their chromatographic behaviour and their sensitivity towards physiological concentrations of hemin (5-10 microM). Further purification on phosphocellulose, however, reveals that the eIF-2 alpha kinase from uninduced MEL cells is chromatographically distinct from HCI, whilst the eIF-2 alpha kinase activity from induced MEL cells represents a mixture of the former and the HCI-type eIF-2 alpha kinase. The latter inhibits protein synthesis in a fractionated system from rabbit reticulocytes which is free of, but sensitive to, HCI, whereas the eIF-2 alpha kinase from uninduced MEL cells does not show any inhibitory activity. This observation is supported by the finding that induced MEL cells respond in vivo to iron depletion with a shut-off of protein synthesis (as do rabbit reticulocytes), whilst uninduced MEL cells do not.

Animals↗
PubMed1989

Xanthogranulomatous pyelonephritis.

Four patients with xanthogranulomatous pyelonephritis are presented; all patients were male and their symptoms were not urinary but nonspecific. Three of the patients are from a low socioeconomic class and did not have adequate medical services; therefore, we suggest that the disease is underreported.

Child, Preschool↗
PubMed1987

[Skin changes in chronic alcoholism with special reference to the zinc and biotin content of the serum].

We report on 52 dermatologic patients suffering from chronic alcoholism. The skin changes observed mainly consisted of erythrosis interfollicularis colli, icterus of the sclerae, teleangiectatic reddening of the face, and diminished secondary hair. In 29 out of 44 sera investigated, we found elevation of the gamma-glutamyl transferase. In 17 patients, serum samples were analyzed for zinc. In 16 cases, the zinc levels were slightly decreased but still within the normal range, i.e. below 100 micrograms/dl. The biotin levels in the 48 serum samples examined varied very much. In 6 patients, however, we found definitely lowered values. Our results support the view that routine investigation of serum zinc and biotin levels might be valuable in patients suffering from chronic alcoholism associated with cutaneous changes. If lowered values are found, a substitution therapy might be of benefit at least in some patients.

Adult↗
PubMed1987

Serum CA 125 in epithelial ovarian cancer. A longitudinal study.

Plasma levels of CA 125 were determined in 113 patients with ovarian cancer of epithelial origin. Of these, 69 patients had CA 125 measured before the first laparotomy and 84.6% of them had a CA 125 level greater than 35 U/ml. In 87 of the 113 patients whose tumour was producing CA 125, a good correlation was observed between the CA 125 levels and the clinical follow-up: 95.7% of the patients in remission had levels less than 35 U/ml, whereas all the patients with no change or with a progressive disease had levels greater than 35 U/ml. Furthermore in recurrent disease the levels of CA 125 were also increased (greater than 35 U/ml) in 92.3% of the patients. Thus, CA 125 measurements at regular intervals are of great clinical value in following the evolution of a tumour or the success of a therapy, but unfortunately do not allow detection of an ovarian tumour at an early stage.

Adenocarcinoma↗
PubMed1987

[The contraction stress test by nipple stimulation: an alternative to the oxytocin test].

Breast stimulation as a "contraction stress test" has been proposed as an alternative test to oxytocin to check the well being of the fetus. 162 breast stimulation tests were carried out in 146 patients with an indication for a "contraction stress test". The stress rate was 66.6%. 8% of the tests showed hyperstimulation without any fetal complications. There was no difference between the types of patients where the test was successful, failed or resulted in hyperstimulation. Where the tests failed, oxytocin tests were carried out. The time needed for these was, on the whole, double that taken for the breast stimulation tests. The two techniques can be comparable, both from correlation of the results with the indication and the outcome for the infant. The mothers tolerated the breast stimulation test very well indeed.

Adult↗
PubMed1986

A (re)initiation-dependent cell-free protein-synthesis system from mouse erythroleukemia cells.

Cultured mouse erythroleukemia cells (MEL cells) can be induced in vivo to erythroid differentiation which is marked by the onset of globin mRNA and haemoglobin synthesis. When these cells are briefly exposed to hypertonic growth medium prior to lysis, the resulting post-mitochondrial supernatants show a high in vitro protein-synthesis activity. Amino acid incorporation is linear up to 60 min; more than 80% of this is due to (re)initiation, as shown by the inhibition with edeine. Extracts from induced cells reach only a third of overall incorporation as compared to extracts from uninduced cells. This reduction of the protein-synthesizing capacity is also observed in vivo. Polyacrylamide gel electrophoresis shows that extracts from uninduced cells faithfully translate their endogenous mRNA, whereas in extracts from induced cells, non-globin protein synthesis is reduced and globin is preferentially synthesized. Haemin (40 microM) as well as purified eukaryotic initiation factor 2 (eIF-2) from rabbit reticulocytes enhance amino acid incorporation in both kinds of extracts, which suggests that both uninduced and induced MEL cells contain a haemin-controlled eIF-2 alpha kinase. This system should be useful for studying the mechanisms controlling protein synthesis in a nucleated differentiating cell.

Animals↗