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Biomedical subjects

M Back

Publications and source records attributed to M Back.

26 records · Page 2Linked to original sources
PubMed1994

State association-sponsored program for SBB candidates.

Concern about the closure of the only Specialist in Blood Banking (SBB) School in the State of Michigan prompted the Michigan Association of Blood Banks (MABB) to develop and administer a comprehensive series of lectures to meet the needs of technologists seeking SBB certification via the experiential route. The program consisted of more than 60 1(1/2)-hour lectures covering all aspects of blood banking (genetics, coagulation, immunology, blood group serology, blood collection and administration, and transfusion medicine) except infectious disease testing and management issues. The latter were presented during two free-standing one-day seminars (for which a fee was charged for those not registered for the lecture series). Educational techniques were covered in a self-study module. Two lectures were presented one morning each week from March through December, with no presentations during July. Lecturers were local volunteer experts, and detailed learning objectives were established for each topic. Each registrant was charged a nominal fee ($100 for MABB members; $150 for nonmembers). There were 32 registrants for the lecture series, with additional registrants for the seminars. Total revenue was $5,100, and expenses amounted to approximately $2,400, mostly for faculty honoraria and catering for the two seminars. The popularity of our program reflects the need for alternatives to formal SBB schools.

Journal Article↗
PubMed1989

Neural plasticity in vivo: opioid sensitivity of memory develops gradually after a septal lesion.

Neuronal plasticity can manifest itself in alterations in the sensitivity of memory to the effects of drugs. After the production of a brain lesion, the memory processing of a passive-avoidance task in mice gradually becomes sensitive to the effect of morphine, i.e., an improvement in retention performance is seen after 6 weeks, but not after 1 or 2 weeks. The results presented demonstrate that, even if they lead to no discernible changes in behaviour, plastic processes can still be detected by means of behavioural tests.

Animals↗
PubMed1988

An amplification technology for improving sensitivity when measuring components in biological samples.

A new technology for improving the sensitivity in measuring components in biological samples is described. The method is based on the use of spherical microbeads (detection beads) which contain a large amount of immobilized enzyme and a reagent with biospecific affinity for the component to be detected. These microbeads have been used in a 'sandwich reaction' for visualization of P-fimbriated Escherichia coli which has a known receptor structure (Gal(alpha 1-4)Gal(beta)). In the initial step the bacteria were enriched on a solid support (e.g., a plastic film or beads (greater than 150 microns)) to which the receptor structure had been covalently bound. In the next step detection beads coupled with enzyme and receptor structure were added and finally a chromogenic substrate for the enzyme was used for visualization. A sensitivity of 10(5) bacteria/ml was reached. The detection beads are of general utility and might be useful for the detection of lectins on other pathogens.

Agglutination Tests↗
PubMed1985

Effects of acetoxycycloheximide and anisomycin on approach and escape responses to hypothalamic stimulation.

The effects of acetoxycycloheximide (AXM) (7 mg/kg) and anisomycin (ANI) (25 and 150 mg/kg) on approach and escape responses induced by hypothalamic stimulation were studied in a situation in which male Swiss mice could initiate a continuous brain stimulation train by pressing a lever at one end of a box (ON-lever) and terminate this stimulation train by pressing a lever at the other end of the box (OFF-lever). AXM and ANI induced a large decrease in ON-OFF responses regardless of the level of stimulation presented (a low level just above the stimulation threshold and a high level which was twice the low level). Correspondingly, a significant increase was observed in the mean time during which animals remained unstimulated (NST) whereas mean time during which animals remained stimulated (ST) increased only slightly. The relative NST increase after treatment was always greater than the relative ST increase either at sites where before treatment NST was greater than ST or at sites where NST was shorter than ST. However, the animals in this latter electrode placement group (ST greater than NST) continued to shuttle and to make lever-pressing responses on the ON-lever, whereas the animals in the other placement group (ST less than NST) did not. These results are discussed in terms of possible interactions between disruptions of reinforcing systems induced by protein inhibitors and their effects on memory.

Animals↗
PubMedDate not supplied

Eccentric iliac artery stenosis: a canine model for angioplasty.

A canine model was developed to induce eccentric iliac artery stenosis. Lesions were induced by exposing the medial aspect of the right and left common iliac arteries in four animals to direct argon laser energy (LA). A 2.5-mm diameter side-firing laser probe was used to deliver 10 W of power in 5-s intervals. Twenty-five pulses were delivered over a 5-cm length of artery with an approximate spot size of 0.8 mm2. Selected arteries (n = 2, LA + Ca) were injected in the same laser injury site with calcium chloride (10% solution). Injections were accurately accomplished using an angioscopically guided guidewire/needle device. In all cases, the internal elastic lamina was broken with focal disruptions of the media. At 9 and 14 days, the LA (n = 3) and LA + Ca vessels (n = 1) had minimal stenoses (< 5% area reduction). These vessels contained edematous media with replacement of necrotic smooth muscle by fibrous tissue. At 23 days, the LA (n = 1) and LA + Ca (n = 1) vessels contained 22% and 33% luminal reductions, respectively, with moderate neointimal proliferation and no evidence of calcification. At 56 days the LA vessels (n = 2) contained 30 and 42% luminal area stenoses with marked neointimal hyperplasia. This preliminary data suggests that controlled arterial wall injury with LA irradiation can induce eccentric fibrointimal lesions in a canine model.

Angioplasty↗