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Biomedical subjects

M B Shtark

Publications and source records attributed to M B Shtark.

At least 37 records · Page 2Linked to original sources
PubMed1993

[The effect of monoclonal antibodies on evoked potentials and on the development of long-term posttetanic potentiation of the hippocampus].

A panel of hybridoma clones producing monoclonal antibodies (Mabs) to the antigens of synaptosomes from rat brain cortex was generated. In immunocytochemical experiments 5F5-B6 Mabs stained stratum moleculare in hippocampus and cerebellum of adult rats intraventricular introduction of 5F5-B6 Mabs was shown to block LTP formation in fascia dentata and to change the evoked potential and population spike amplitude.

Animals↗
PubMed1991

Scientific and clinical biofeedback in the USSR.

This paper describes the history of biofeedback research and application in the USSR. The approach of USSR scientists to the study of basic mechanisms of biofeedback and the status of clinical applications of biofeedback in the Soviet Union are described. Trends in the publication of biofeedback-related papers in Russian are presented, and the present "state of the art" described. Promising prospects for biofeedback research and application are discussed.

Biofeedback, Psychology↗
PubMed1990

[Monoclonal antibodies to individual antigens of the nervous system of the snail, Helix pomatia].

Seven positive hybridoma clones were chosen by immunoenzyme analysis amons 103 clones obtained by hybridization of NSO plasmocytoma cells and splenocytes from BALB/C mice, immunized with snail's nervous system antigens. Specific binding of Mabs with neuron cytoplasmic antigens was indicated on cryostat sections of visceral, pedal and cerebral ganglia. The Mabs obtained could be used for the study of physiological role of antigens identified.

Animals↗
PubMed1990

[The mechanisms regulating pulse-wave spreading time in a biological feedback regimen in patients with borderline arterial hypertension and in healthy subjects].

The regulatory mechanisms of pulse transit time (PTT) during biofeedback training were studied by noninvasive cardiovascular monitoring in borderline hypertensive and normotensive subjects. Hemodynamic parameters were used as indirect indices of autonomous nervous system activity. All the patients were capable of increasing PTT. Systolic blood pressure demonstrated significant correlation with PTT. The effects were mediated by the different combinations of sympathetic and parasympathetic drives. In the initial 5 sessions, there was the most significant decrement of sympathetic, especially beta-adrenergic activity, expressed in the reduction of the myocardial contractility and cardiac output. In the retraining period (6 to 12 sessions), the leading role was played by the reduction of the arterial tone and total peripheral resistance. Respiratory arrhythmia rose in both cases as a result of the increased parasympathetic activity.

Adult↗
PubMed1989

[A microcomputer system of biotechnical feedback].

A microcomputer system has been constructed and developed for biofeedback research and clinical biofeedback training. Hardware and software enable on-line data acquisition, storage and real-time analysis with visual feedback of chosen parameters, while changes in other physiological variables are recorded and monitored. The software handles control of experimental sessions, data reduction, statistics, data base management and output document forming. Computer analysis of pulse transit time biofeedback training with multiple cardiovascular monitoring in experimental and clinical setting outlined contributions of sympathetic and parasympathetic influences on observed patterns of cardiovascular modifications.

Biofeedback, Psychology↗
PubMed1988

[Electrophysiological and neurochemical study of long-term sensitization in the snail].

The long-term sensitization of avoidance reflex was produced in snail Helix pomatia, which led to the remarkable increase in the pneumostome closures period. The formation of long-term sensitization is also accompanied by increase in excitability of command neurons of this reflex. One of the possible mechanisms of this phenomenon is the depolarization of these cells. The quantitative redistribution of water-soluble proteins with relative mobility 0.54 0.42 0.40 was also observed in the identified neurons, both included in the avoidance reflex (command neurons) and non-included (bursting neurons, nerve cells of pool D). The protein with the relative mobility of 0.75 was unique for the nerve cells of neurosecretory pool D in sensitized snails, and was never found in control animals.

Action Potentials↗
PubMed1988

Alterations in calmodulin and S-100 protein content of hippocampal slices during long-term potentiation.

The content of calmodulin and S-100 protein in fractions of rat hippocampal slices was assayed by solid phase radioimmunology and radial immunodiffusion, respectively. One hour after tetanization (electrical stimulation of area dentata granular cells and recording from CA3 pyramids) an inverse translocation of these Ca++-binding proteins was observed: an increase in the calmodulin content in the water-soluble and a decrease in the Lubrol-soluble fractions, while an increase in S-100 protein in the Triton-soluble and a decrease in the water-soluble fractions occurred. The results are suggestive of a regulatory function of these proteins in events during repetitive stimulation of a synaptic input. The calmodulin increase in the cytosolic compartment may reflect the involvement of Ca++-calmodulin dependent intraneuronal metabolic processes underlying the induction and/or temporary maintenance of neuronal functional changes occurring after repeated or intense synaptic activity. The elevated S-100 protein level in the membrane compartment might be interpreted in terms of functionally induced redistribution in that neuronal cells are provided with additional amounts of S-100 protein originating from the surrounding glial cells which store large amounts of soluble S-100 protein.

Animals↗
PubMed1985

Hibernators' brain: protein synthesis in the neocortex and the hippocampus.

[3H]leucine incorporation into 16 electrophoretic protein fractions from the neocortex and the hippocampus and their relative content have been estimated in various physiological states of ground squirrels: in torpor, in 3 transition phases, in long-term wakefulness and a fortnight after forced arousal in winter. The changes are greater in the neocortex than in the hippocampus. Incorporation is not the same in naturally awake animals and forced awake animals.

Animals↗
PubMed1985

[Microchromatographic study of proteins in hippocampal field CA3 during prolonged posttetanic potentiation in surviving sections].

A study was made of 3H-leucine incorporation into water- and tween-soluble proteins of the hippocampal CA3 field under the development of prolonged post-tetanic potentiation (PPTP) because of stimulation of mossy fibers. The mixture of supernatant proteins was assayed by ion-exchange microchromatography while each fraction was evaluated for the radioactive label incorporation. In the course of the assay of the chromatograms obtained, no significant alterations in the protein spectrum were found during the development of the PPTP. Substantial changes were identified in the rate of the labeled leucine incorporation in the acid part of the spectrum represented by acid proteins and polypeptides including brain specific ones. It is assumed that the development of the PPTP in the hippocampal pyramids is associated with activation of the proteins under consideration.

Animals↗
PubMed1984

[Water-soluble proteins of the subesophageal ganglionic complex in the edible snail in the early stages of learning].

During the early stages of conditioned defensive reflex formation, a dramatic increase in the acidic protein concentration can be seen in the snail CNS (the mobility 0.58 in polyacrylamide gel) with reference to water-soluble proteins of the animals' ganglions under study. Such an increase occurs at the time when the majority of neurons are involved in the learning process. The rise of the protein content is also seen upon the snail arousal, 2-6 h after being awake. It is suggested that the high level of the protein correlates with the appearance of the orientation reflexes. At the cellular level this phenomenon appears to be linked with increased excitability of a great number of neurons.

Animals↗
PubMed1983

[Effect of D,L-dopa on the content of protein antigens in rat brain structures].

Cross immunoelectrophoresis was used to study the effect of administering the noradrenaline precursor D,L-DOPA on the content of water-soluble antigens in the hypothalamus, cerebellum and frontal cortex of rats. The content of 3 out of the 10 antigens under study significantly increased in the hypothalamus and that of 2 in the cerebellum after intraperitoneal injection of D,L-DOPA. The content of these antigens was greater in the structures with a high noradrenaline level.

Animals↗
PubMed1982

[Neurospecific antigen D in the postnatal ontogeny of rat brain structures].

The time course of antigen D similar to well-known neurospecific proteins 14-3-2 and antigen alpha was studied during postnatal ontogenesis of brain structures of Wistar rats: Cross immunoelectrophoresis was made use of to measure the content of antigen D in the visual cortex, hippocamp and nucleus caudatus of rats aged 7, 14, 21 and 30 days and 1 year. Changes in the levels of two antigen D components, slow- and fast-migrating (SMC and FMC) were recorded to show that their content in the test structures increased most intensely from the 14th to the 21st day of postnatal development. In all the brain structures of tendency was recorded towards the increased SMC/FMC ratio during postnatal ontogenesis, that may point to an important role of FMC for bain functions in maturing and adult animals.

Aging↗
PubMed1981

S100, a brain-specific protein: localization and possible role in the snail nervous system.

Immunofluorescence techniques were used to show that S100 is present on the surface of neuronal and glial membranes of Helix pomatia in vitro. By the method of rocket immunoelectrophoresis of aqueous , Trition, and n-pentanol extracts of snail nervous tissue, S100 was demonstrated to be mainly in the membrane fraction. Anti-S100 antiserum inhibited the electrical activity of identified neurons, pointing to a relationship of this process with ionic channels of the excitable membrane. The effect of anti-S100 antiserum on the membrane was potential dependent and controlled by the Ca2+ concentration.

Action Potentials↗
PubMed1979

[Localization of neurospecific protein-antigens on the surface membrane of neurons and glia of grape shails].

The existence of cross (common) proteins-antigens on the membrane of nervous and glial cells of Helix pomatia in vitro was proved by Coons' method of immunofluorescence. The presence of the neurospecific protein S-100 on the membrane of these cells and antigenous heterogeneity of the membranes of the neuronal cell population were established. The antigen concentration was shown to vary on the somatic and axonal membranes. The distribution pattern of specific luminescence indicates potential qualitative and/or quantitative differences in the content of neurospecific proteins in various portions of the neuronal membrane.

Animals↗