Search PubMed⌕ Search

Biomedical subjects

M B Markus

Publications and source records attributed to M B Markus.

At least 55 records · Page 3Linked to original sources
PubMed1990

A survey of the intestinal nematodes of Bushmen in Namibia.

Studies in Namibia revealed prevalence rates of 63% for hookworm and 35% for Trichuris in 31 Bushmen, 4-65 years of age, who were encamped in the Kaudom Game Reserve. The study also revealed prevalence rates of 85% for hookworm, 25% for Strongyloides, and 1% for Trichuris in 103 children, 6-17 years of age, attending 5 schools in Bushmanland. The 25% Strongyloides infection rate is one of the highest prevalences recorded in southern Africa. The Trichuris infections in the Kaudom group appeared to be autochthonous, and this population did not harbor Strongyloides. Regular contact with pans in summer and boreholes at any time probably facilitated the acquisition of infections in wet and fecally polluted sites. Thirteen spurious Physaloptera infections were recorded.

Adolescent↗
PubMed1990

Typing of southern African isolates of Plasmodium falciparum using monoclonal antibodies.

Antigenic diversity among 19 southern African isolates of Plasmodium falciparum was demonstrated using a panel of 9 monoclonal antibodies. Parasites obtained from single patients were heterogeneous. The antigen composition of 9 isolates was not stable with time in culture, particularly not with respect to 4 of the monoclonal antibodies. By the end of the investigation, 70% of isolates displayed an identical antigen pattern which was markedly different to any obtained in other parts of the world. Differences may be due to geographic origin of parasites or to variation in culture conditions.

Africa, Southern↗
PubMed1988

In vitro confirmation of chloroquine-resistant Plasmodium falciparum malaria in KwaZulu.

In May 1987 and January 1988 the chloroquine sensitivity of Plasmodium falciparum in the Ubombo and Ingwavuma districts of KwaZulu was determined by a modified in vitro microtest in which the patients' plasma was replaced with non-immune human AB serum and the test plates were incubated in an atmosphere of 3% oxygen, 4% carbon dioxide and 93% nitrogen. A success rate of 74% was achieved using this technique. All of 23 successfully tested isolates from malaria patients reporting to clinics and a hospital in these areas were found to be resistant to chloroquine, schizogony being inhibited at 32 pmol per well in the majority of tests. Seventy-five per cent of the isolates obtained through active surveillance in the Ubombo district were found to be resistant in varying degrees. Malarial parasites collected from clinics and a hospital in the endemic area did not change markedly in their in vitro response to chloroquine during the 8-month period May 1987-January 1988.

Animals↗
PubMed1988

In vitro cultivation of southern African strains of Plasmodium falciparum and gametocytogenesis.

Sixteen southern African isolates of Plasmodium falciparum were successfully established and maintained in gassed tissue culture flasks which were either held in a static position or shaken. Shaken cultures yielded 48-hour growth rates ranging from 3.9-fold to 9.5-fold and peak parasitaemias ranging from 9% to 27%. Growth rates in static flasks were lower, ranging from 1.8-fold to 4.2-fold every 48 hours with peak parasitaemias ranging from 4% to 12%. Four of the established isolates were treated so as to promote gametocytogenesis and the rate of gametocyte production was found to be high. The mean percentage gametocytes by day 20-25 was 4.2% of erythrocytes, which represented approximately 50% of total parasites.

Animals↗