Biomedical subjects

M B Ducharme

Publications and source records attributed to M B Ducharme.

36 records · Page 2Linked to original sources

PubMed1996

The effect of postural changes on body temperatures and heat balance.

Early studies have demonstrated that rectal temperature (T(re)) decreases and mean skin temperature (Tsk) increases in subjects changing their posture from standing to supine, and vice versa. Such changes have important implications insofar as thermal stress experiments are conducted and interpreted. However, the extent of these changes between steady-state conditions is not known. In addition, it is not known whether thermal balance is also affected by postural changes. To examine these questions, 11 healthy males were exposed to a thermoneutral air environment (28.2-28.5 degrees C and 40% relative humidity) in various postures at rest. Body temperatures, heat losses, and metabolic rate were measured. Subjects wore shorts only and began in an upright posture (standing or sitting at an inclination of 7.5 degrees) on a customized tilt-table. They were tilted twice, once into a supine position and then back to the original upright position. Each tilt occurred after steady state was satisfied based on the subject's circadian variation of T(re) determined previously in a 4.25 h control supine trial. Times to supine steady state following the first tilt were [mean(SE)] 92.6 (6.4) and 116.6 (5.1) min for the standing and sitting trials, respectively. Times to upright steady state following the second tilt were 107.9 (11.4) and 124.1 (9.0) min. Mean steady-state T(re) and Tsk were 36.87 (0.07) and 34.04 (0.14), 37.47 (0.09) and 33.48 (0.14), and 37.26 (0.05) and 33.49 (0.10) degrees C for supine, standing, and sitting, respectively. Thermal balance was attained in all steady-state conditions, and allowing for a decrease in the weighting factor of T(re) for mean body temperature in the upright postures, it also appears that thermal balance was preserved between changes in posture. These results are consistent with no perceived changes by the subjects in their thermal comfort and skin wetness.

Adult↗

PubMed1996

Influence of granisetron on thermoregulation during exercise in the heat.

BACKGROUND: A NATO project group has an interest in selecting an antiemetic agent that not only is effective in the prevention of emesis induced by chemical agents or radiation exposure but also has minimal, if any, side effects. Granisetron is the second candidate drug of a class of selective serotonin antagonists that has been shown to be an effective antiemetic agent for patients receiving radiation or chemotherapy treatment. The present study was designed to evaluate whether a single 2-mg oral dose of granisetron influenced temperature regulation during exercise in a hot and relatively dry environment. HYPOTHESIS: Based on our previous findings with the other candidate drug, ondanseton, we hypothesized that granisetron would not influence temperature regulation. METHODS: Nine unacclimatized males performed a drug and placebo trial in a double-blind manner. The sessions involved walking on a treadmill at 4.8 km.h-1 with a 2% elevation for a maximum of 3 h at 40 degrees C and 30% relative humidity while wearing combat clothing. RESULTS: Granisetron was associated with a small (0.2 degrees C) but significant elevation in mean skin temperature at the beginning and after 2 h of exercise. However, there was no difference between trials for the 1.6 degrees C increase in rectal temperature. Also, body heat gain (406 +/- 97 and 407 +/- 103 k) for the placebo and drug trial, respectively) and whole body sweat rates (0.72 +/- 0.10 and 0.73 +/- 0.10 kg.h-1 for the placebo and granisetron trial, respectively) were not different. Tolerance times also were not different for the placebo (157.4 +/- 16.7 min) and drug (159.4 +/- 20.4 min) sessions. CONCLUSIONS: For the environmental conditions used in this investigation, we would accept the null hypothesis that a single 2 mg oral dose of granisetron does not influence temperature regulation during exercise.

Antiemetics↗

PubMed1996

Granisetron shows no pro-arrhythmic effect in normal subjects during or after exercise in a hot environment.

BACKGROUND: 5-HT3 receptor antagonists are being evaluated as possible agents to prevent nausea and vomiting associated with radiation exposure in a non-clinical military setting. Because of concern about potential cardiovascular toxicity and the observation that certain developmental 5-HT3 antagonists produced undesirable effects, all drugs in this class are being carefully scrutinized for possible adverse cardiac effects. METHOD: In this study, nine subjects underwent ambulatory ECG monitoring for an average of 21.6 h after a 2-mg oral dose of granisetron or placebo in a double-blind crossover protocol. Monitoring included a 3-h period of submaximal exercise in a 40 degrees C environment. RESULTS: Although isolated ventricular and supraventricular ectopic activity, sinus bradycardia, and pauses were found, there were no sustained arrhythmias observed in either the placebo or granisetron conditions. CONCLUSION: Although the generalizability of this study is limited by the small number of subjects, these observations add to the body of evidence confirming the lack of cardiovascular toxicity of granisetron.

Administration, Oral↗

PubMed1995

Effect of triazolam on responses to a cold-water immersion in humans.

Managing alertness of soldiers during sustained operations is a source of serious concern for military unit commanders. A frequently employed strategy is to induce sleep before an operation, especially operations requiring prolonged travel. Sleep-inducing drugs could have an action on thermoregulation through their effect on alertness and a possible direct effect on the brain. The goal of this study was therefore to evaluate the effect of a commonly prescribed triazolam (Halcion) on thermoregulatory responses to cold-water immersion. Eight subjects were immersed twice in 18 degrees C water for up to 90 min in the morning; once after ingesting 0.25 mg triazolam (TRZ) the prior evening, and again after placebo (PLB) treatment. There were no significant differences between trials for mean duration of the immersion, the change in rectal temperature and mean skin temperature. Total metabolic heat production was similar for both conditions: 767 +/- 107 vs. 781 +/- 105 kj.m-2 for TRZ and PLB, respectively. The results should be considered in light of a large variation among the subjects in sensitivity to TRZ, which was unrelated to biometrical characteristics such as surface area-to-mass ratio, lean body mass, % body fat, and physical fitness. Although not statistically significant, there was a trend for a smaller increase in plasma free fatty acid and glycerol concentrations after water immersion with TRZ. The results suggest that the ingestion of a single dose of triazolam 11 h prior to a cold-water immersion is not likely to accelerate the rate of onset of hypothermia. Individual sensitivity, however, may predispose some sensitive subjects to negative effects in this regard.

Adipose Tissue↗

PubMed1994

Comparison of forced-air patient warming systems for perioperative use.

BACKGROUND: Perianesthetic hypothermia is common and produces several complications, including postoperative shivering, decreased drug metabolism and clearance, and impaired wound healing. Forced-air warming transfers more than 50 W to the body and is an efficient method for either preventing or reversing decreases in core temperature. METHODS: The authors compared the efficacy of four complete forced-air warming systems: (1) Bair Hugger 250/PACU Patient Warming System with 300 Warming Cover (Augustine Medical, Eden Prairie, MN); (2) Thermacare TC1000 Power Unit with TC1050 Comfort Quilt (Gaymar Industries, Orchard Park, NY); (3) WarmAir 130 Hypothermia System with 140 Warming Tube (Cincinnati Sub-Zero Products, Cincinnati, OH); and (4) WarmTouch 5000 Patient Warming System and 503-0810 CareQuilt (with the connecting hose compressed [short] and extended [long]) (Mallinckrodt Medical, St. Louis, MO). Six minimally clothed male volunteers were studied supine in a 24.5 degrees C environment. Cutaneous heat flux and skin temperature was measured at 14 area-weighted sites using thermal flux transducers. After 20-min control periods, volunteers were warmed for 40 min in each condition. A cotton blanket was placed over each cover. Power units were placed at the foot end of the bed, started cold, and set at maximum temperature and flow settings. All units reached maximum efficiency within 20 min. RESULTS: Total heat transfer with the Bair Hugger system (95 +/- 7 W) was greater (P < 0.05) than with WarmTouch (short hose 81 +/- 6 W and long hose 68 +/- 8 W), Thermacare (61 +/- 5 W), and WarmAir (38 +/- 6 W) systems. Each cover also was tested on a common power unit (Bair Hugger 200). Total heat transfer was greater (P < 0.05) with the Warming Cover (Bair Hugger) (88 +/- 8 W), followed by the Comfort Quilt (Thermacare) (56 +/- 6 W), CareQuilt (WarmTouch) (50 +/- 7 W), and the Warming Tube (WarmAir) (43 +/- 6 W). CONCLUSIONS: The advantages of the Bair Hugger system and Warming Cover are evident in areas that are important for heat transfer from the periphery to the body core (chest, axilla, abdomen, and upper legs).

Adult↗

PubMed1994

Role of blood as heat source or sink in human limbs during local cooling and heating.

The objective of the present study was to investigate the relative contribution of the convective heat transfer in the forearm and hand to 1) the total heat loss during partial immersion in cold water [water temperature (Tw) = 20 degrees C] and 2) the heat gained during partial immersion in warm water (Tw = 38 degrees C). The heat fluxes from the skin of the forearm and finger were continuously monitored during the 3.5-h immersion of the upper limb (forearm and hand) with 23 recalibrated heat flux transducers. The last 30 min of the partial immersion were conducted with an arterial occlusion of the forearm. The heat flux values decreased during the occlusion period at Tw = 20 degrees C and increased at Tw = 38 degrees C for all sites, plateauing only for the finger to the value of the tissue metabolic rate (124.8 +/- 29.0 W/m3 at Tw = 20 degrees C and 287.7 +/- 41.8 W/m3 at Tw = 38 degrees C). The present study shows that, at thermal steady state during partial immersion in water at 20 degrees C, the convective heat transfer between the blood and the forearm tissue is the major heat source of the tissue and accounts for 85% of the total heat loss to the environment. For the finger, however, the heat produced by the tissue metabolism and that liberated by the convective heat transfer are equivalent. At thermal steady state during partial immersion in water at 38 degrees C, the blood has the role of a heat sink, carrying away from the limb the heat gained from the environment and, to a lesser extent (25%), the metabolic and conductive heats. These results suggest that during local cold stress the convective heat transfer by the blood has a greater role than that suggested by previous studies for the forearm but a lesser role for the hand.

Adolescent↗

PubMed1994

Influence of ondansetron on thermoregulation during exercise in the heat wearing combat clothing.

Ondansetron is a selective serotonin antagonist which has been shown to be an effective antiemetic agent for patients undergoing radiation or chemotherapy treatment. The Canadian Forces together with other NATO Countries have an interest in selecting an antiemetic agent that not only is effective in the prevention of emesis induced by chemical agents or radiation exposure, but also has minimal, if any, side effects. The purpose of this study was to examine the influence of a single 8-mg oral dose of the drug on thermoregulation during exercise in a hot (40 degrees C, 30% relative humidity) environment. Ten unacclimatized males performed a drug and placebo trial in single-blind random order. The sessions involved walking on a treadmill at 4.8 km.h-1 with a 2% elevation for a maximum of 3 h. Subjects wore combat clothing during the trials. Total exposure time was similar for the placebo (177 +/- 6 min) and drug (172 +/- 11 min) trials. Also, the rate of sweat production (0.64 +/- 0.1 and 0.66 +/- 0.1 kg.h-1 for placebo and drug, respectively) and body heat gain (303 +/- 112 and 305 +/- 110 kj for the placebo and drug, respectively) were not different between trials. Rectal temperature increased 1.48 +/- 0.40 degrees C for the placebo and 1.47 +/- 0.37 degrees C for the ondansetron trial. Finally, there was no difference in the mean skin temperature response which increased in both conditions to 37.1 +/- 0.5 degrees C. Under the conditions of this experiment, there is no evidence to suggest that the ingestion of ondansetron influences thermoregulation in a hot environment.

Adult↗

PubMed1993

Is energy substrate mobilization a limiting factor for cold thermogenesis?

Energy substrate mobilization has been suggested as being a limiting factor for the rate of cold-induced thermogenesis (M), and consequently in delaying hypothermia. The evidence supporting this hypothesis in humans, however, is not convincing and the hypothesis has yet to be tested in a rigorous manner using a full heat balance analysis (partitional calorimetry). The goal of this study was therefore to re-investigate whether enhancing energy substrate mobilization by feeding cold-exposed subjects would improve M and affect heat debt (S; the minute-by-minute balance of M and heat losses) as well as rectal (Tre) and mean skin temperatures (Tsk). Nine healthy semi-nude fasted subjects were exposed to 5 degrees C (3 h at rest, 1 m.s-1 wind) on three occasions following the ingestion at min 0 and 90 of either: (1) a placebo, (2) 710 kJ of pure carbohydrates (100%-CHO), or (3) 710 kJ of a high-carbohydrate bar (High-CHO). As expected in the cold, Tre and Tsk decreased whereas M, S and heat losses increased (P < 0.01). However, there were no differences between treatments, including the final Tre [mean (SEM); 36.4 (0.2); 36.5 (0.3) and 36.5 (0.2) degrees C for the placebo, 100%-CHO and High-CHO tests, respectively]. During the 100%-CHO treatment, rates of carbohydrate oxidation were the highest and fat oxidation the lowest (P < 0.05), whereas the High-CHO treatment caused smaller changes.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗

PubMed1993

Heat flux transducer measurement error: a simplified view.

Heat flux transducers (HFTs) provide a simple and direct measurement of body heat exchange. Regrettably, HFTs perturb the heat flux at the measurement site, resulting in underestimations of the true heat flux. Equations to correct the discrepancy are available, but most require high-precision temperature measurements above and/or below the transducer and/or deep within the body tissues. Because this is not always feasible, the equations are of limited practical benefit. A theoretical basis for the magnitude of the correction factor in relation to the thermal resistances of the materials both above and below the HFT has been developed and has been verified experimentally. The theory is presented in a graph that can be used to drive the HFT correction factor directly or as a guide to know that heat flux was measured within a certain accuracy. This may obviate the use of complicated procedures and equations to perhaps needlessly apply a small correction factor to HFT data.

Biophysical Phenomena↗

PubMed1993

Adequacy of food rations in soldiers during an arctic exercise measured by doubly labeled water.

To investigate the adequacy of food rations to supply energy needs in cold-temperature environments, caloric expenditure and intake and body composition changes were measured in a group of infantrymen during a 10-day field exercise in the Canadian Arctic. Energy expenditure was measured by the doubly labeled water method (n = 10), and caloric intake was measured by complete food intake records (n = 20). Body composition was determined by isotope dilution (n = 10) and bioelectrical impedence analysis (n = 20) on days 0 and 10. Baseline isotopic enrichment shifts due to geographical relocation were also monitored (n = 5). Mean body weight decreased 0.63 +/- 0.83 (SD) kg over the study period (P < 0.005), although fat-free and fat mass compartment changes were not significant. Baseline isotopic changes were -4.65 +/- 2.54 and -0.48 +/- 0.07 /1000/day for deuterium and 18O, respectively. Mean baseline corrected energy expenditure level was 4,317 +/- 927 kcal/day. Self-reported caloric intakes obtained from food records were 2,633 +/- 499 kcal/day (61.0% of expenditure). Rations packs contained 4,350 kcal/day. Results suggest that 1) food intake was significantly underreported and 2) the energy needs of most subjects were being met by rations and available supplements.

Arctic Regions↗

PubMed1992

Forearm temperature profile during the transient phase of thermal stress.

The transient temperature response of the resting human forearm immersed in water at temperatures (Tw) ranging from 15 to 36 degrees C was investigated. Tissue temperature (Tt) was continuously monitored by a calibrated multicouple probe during the 3-h immersions. Tt was measured every 5 mm, from the longitudinal axis of the forearm to the skin surface. Skin temperature, rectal temperature, and blood flow (Q) were also measured during the immersions. The maximum rate of change of the forearm mean tissue temperature (Tt, max) occurred during the first 5 min of the immersion. Tt, max was linearly dependent on Tw (P less than 0.001), with mean values (SEM) ranging from -0.8 (0.1) degrees C.min-1 at 15 degrees C to 0.2 (0.1) degrees C.min-1 at 36 degrees C. The maximum rate of change of compartment mean temperature was dependent (P less than 0.001) on the radial distance from the longitudinal axis of the forearm. The half-time for thermal steady state of the forearm mean tissue temperature was linearly dependent on Tw between 30 and 36 degrees C (P less than 0.01), with mean values (SEM) ranging from 15.6 (0.6) min at 30 degrees C to 9.7 (1.2) min at 36 degrees C and not different between 15 and 30 degrees C, averaging 16.2 (0.6) min. There was a significant linear relationship between the half-time for thermal steady-state of the compartment mean temperature and the radial distance from the longitudinal axis of the forearm for each value of Tw tested (P less than 0.001). The data of the present study suggest that the forearm Q is an important determinant of the transient thermal response of the forearm tissue during thermal stress.

Adult↗

PubMed1991

Cyclic intramuscular temperature fluctuations in the human forearm during cold-water immersion.

The purpose of the present study was to investigate the intramuscular temperature fluctuations in the human forearm immersed in water at 15 degrees C. Tissue temperature (Tt) was continuously monitored by a calibrated multicouple probe during 3 h immersion of the forearm. The probe was implanted approximately 90 mm distal from the olecranon process along the ulnar ridge. Tt was measured every 5 mm, from the longitudinal axis of the forearm (determined from computed tomography scanning) to the skin surface. Along with Tt, rectal temperature, skin temperature and heat loss of the forearm were measured during the immersions. Five of the six subjects tested showed evidence of cyclic temperature fluctuations in the forearm limited to the muscle tissue. The first increase of the muscle temperature was observed 75 (SE 6) min after the onset of the immersion, and the duration of the cycle averaged 36 (SE 3) min. The maximum increase of the muscle temperature, which ranged between 0.4 degrees C and 1.0 degrees C, was measured at the axis of the forearm, and was inversely correlated to the circumference of the subject's forearm (P less than 0.05). No corresponding increases of the skin temperature and heat loss of the forearm were observed for the complete duration of the immersion. These data support the hypothesis of a significant contribution of the muscle vessels during cold-induced vasodilatation in the forearm.

Adolescent↗

PubMed1991

Finite-element solution of thermal conductivity of muscle during cold water immersion.

The in vivo or effective thermal conductivity (keff) of muscle tissue of the human forearm was determined through a finite-element (FE) model solution of the bioheat equation. Data were obtained from steady-state temperatures measured in the forearm after 3 h of immersion in water at temperatures (Tw) of 15 (n = 6), 20 (n = 5), and 30 degrees C (n = 5). Temperatures were measured every 0.5 cm from the longitudinal axis of the forearm to the skin approximately 9 cm distal from the elbow. Heat flux was measured at two sites on the skin adjacent to the temperature probe. The FE model is comprised of concentric annular compartments with boundaries defined by the location of temperature measurements. Through this approach, it was possible to include both the metabolic heat production and the convective heat transfer between blood and tissue at two levels of blood flow, one perfusing the compartment and the other passing through the compartment. Without heat exchange at the passing blood flow level, the arterial blood temperature would be assumed to have a constant value everywhere in the forearm muscles, leading to a solution of the bioheat equation that greatly underpredicts keff. The extent of convective heat exchange at the passing blood flow level is estimated to be approximately 60% of the total heat exchange between blood and tissue. Concurrent with this heat exchange is a decrease in the temperature of the arterial blood as it flows radially from the axis to the skin of the forearm, and this decrease is enhanced with a lowered Tw.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗

PubMed1991

In vivo thermal conductivity of the human forearm tissues.

The effective thermal conductivities of the skin + subcutaneous (keff skin + fat) and muscle (keff muscle) tissues of the human forearm at thermal steady state during immersion in water at temperatures (Tw) ranging from 15 to 36 degrees C were determined. Tissue temperature (Tt) was continuously monitored by a calibrated multicouple probe during a 3-h immersion of the resting forearm. Tt was measured every 5 mm from the longitudinal axis of the forearm (determined from computed-tomography scanning) to the skin surface. Skin temperature (Tsk), heat loss (Hsk), and blood flow (Q) of the forearm, as well as rectal temperature (Tre) and arterial blood temperature at the brachial artery (Tbla), were measured during the experiments. When the keff values were calculated from the finite-element (FE) solution of the bioheat equation, keff skin + fat ranged from 0.28 +/- 0.03 to 0.73 +/- 0.14 W.degrees C-1.m-1 and keff muscle varied between 0.56 +/- 0.05 and 1.91 +/- 0.19 W.degrees C-1.m-1 from 15 to 36 degrees C. The values of keff skin + fat and keff muscle, calculated from the FE solution for Tw less than or equal to 30 degrees C, were not different from the average in vitro values obtained from the literature. The keff values of the forearm tissues were linearly related (r = 0.80, P less than 0.001) to Q for Tw greater than or equal to 30 degrees C. It was found that the muscle tissue could account for 92 +/- 1% of the total forearm insulation during immersion in water between 15 and 36 degrees C.

Adipose Tissue↗

PubMed1991

Tissue temperature profile in the human forearm during thermal stress at thermal stability.

The purpose of the present study was to investigate the effect of a range of water temperatures (Tw from 15 to 36 degrees C) on the tissue temperature profile of the resting human forearm at thermal stability. Tissue temperature (Tti) was continuously monitored by a calibrated multicouple probe during 3 h of immersion of the forearm. The probe was implanted approximately 9 cm distal from the olecranon process along the ulnar ridge. Tti was measured every 5 mm, from the longitudinal axis of the forearm (determined from computed tomography scanning) to the skin surface. Along with Tti, skin temperature (Tsk), rectal temperature (Tre), and blood flow were measured during the immersions. For all temperature conditions, the temperature profile inside the limb was linear as a function of the radial distance from the forearm axis (P less than 0.001). Temperature gradient measured in the forearm ranged from 0.2 +/- 0.1 degrees C C cm (Tw = 36 degrees C) to 2.3 +/- 0.5 degrees C cm (Tw = 15 degrees C). The maximal Tti was measured in all cases at the longitudinal axis of the forearm and was in all experimental conditions lower than Tre. On immersion at Tw less than 36 degrees C, the whole forearm can be considered to be part of the shell of the body. With these experimental data, mathematical equations were developed to predict, with an accuracy of at least 0.6 degrees C, the Tti at any depth inside the forearm at steady state during thermal stress.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗

PubMed1991

Physiological responses of the human extremities to cold water immersion.

Five subjects immersed their hands and feet twice during 1 hour sessions in a calorimeter bath, filled with water at 25 degrees C. Before immersion the hand (33.0 +/- 2.2 degrees C) was warmer than the foot (30.6 +/- 2.1 degrees C) and so was the blood flow: respectively 5.8 +/- 5.2 versus 0.7 +/- 0.3 ml.min-1.100 mo tissue-1 as determined by strain gauge plethysmography and 10.8 +/- 5.1 versus 6.7 +/- 5.3 by the doppler method. At the end of the immersion period the blood flow had decreased a factor of 2.9 (doppler) and 4.3 (plethysmography). The local heat transfer, determined by heat flux transducers, was highest immediately after immersion. The maximum was higher at the ventral sides than at the dorsal sides of the hand (225 +/- 93 versus 186 +/- 61 W.m-2 and the foot (178 +/- 53 versus 160 +/- 57 W.m-2). The total heat transferred to the water by hand or foot during the 60 minutes of immersion, determined by calorimetry, was 47 +/- 21 and 36 +/- 36 kJ respectively and was not statistically different due to considerable interindividual differences. Interindividual differences were also found for heat flux, rectal and local skin temperature and blood flow. These differences will have to be taken into account if a comparison is made with data from subjects with local cold injuries.

Adolescent↗

PubMed1990

Errors in heat flux measurements due to the thermal resistance of heat flux disks.

Questions have been raised regarding the effect of the thermal resistance of heat flux transducers (HFTs) on the thermal flux from the skin. A model capable of simulating a large range of "tissue" insulation (variable-R model) was used to study the effect of the underlying tissue insulation on the relative error in heat flux due to the thermal resistance of the HFTs. The data show that the deviation from the true value of heat flux increases as the insulation of the underlying tissue decreases (r = 0.99, P less than 0.001). The underestimation of the heat flux through the skin measured by an HFT is minimal when the device is used on vasoconstricted skin in cool subjects (3-13% error) but becomes important when used during vasodilation in warm subjects (29-35% error) and even more important on metallic-skin mannequins (greater than 60% error).

Body Temperature Regulation↗

PubMed1988

A multicouple probe for temperature gradient measurements in biological materials.

An easy-to-make, sensitive, thin, flexible, multisensor probe for in vivo tissue temperature profile measurement is described. It is essentially a multijunction thermocouple (i.e., a multicouple) of type-T composition. Enamel-insulated copper wires (38 gauge) were soldered 5 mm apart to one common uninsulated constantan wire (36 gauge) and introduced into a polyethylene tube sealed at one end. The total outside diameter of the multicouple probe is less than 1 mm, and the maximum number of junctions using the specified wire sizes is approximately 16. This design permits the instantaneous measurement of a tissue temperature profile at 5-mm intervals over a distance of approximately 8 cm. An extensive calibration for the thermal conductivity effect (k effect) along the multicouple wires by means of a limb model is presented. The results show that the temperature readings of the individual junctions are significantly affected by the k effect when a thermal gradient exists along the multicouple, as is usually the case during tissue temperature measurements. However, calibration of the multicouple for the k effect yields a measurement accuracy of +/- 0.1 degree C under a wide range of gradients. This probe can be implanted in tissues to measure thermal gradients under different physiological conditions.

Animals↗