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Biomedical subjects

M Aymard

Publications and source records attributed to M Aymard.

At least 145 records · Page 8Linked to original sources
PubMed1983

[Experimental swine influenza. Comparison of the pathogenicity of swine-type wild influenza viruses and strains of human type H3 N2 whose reservoir is swine].

Intra-nasal inoculation of 10-day-old piglets, free of specific pathogenic flora, with swine influenza virus A/H1 N1 and A/H3 N2 produces lesions of viral pneumonia without detectable clinical signs. Following indirect contamination all the strains studied were able to multiply in the respiratory tract; in those cases where no macroscopic lesion was seen, microscopic examination revealed variable modification of the pulmonary tissue depending on the virus causing infection. Influenza virus type H3 N2 are naturally found in many groups of pigs and can therefore play an important role in the respiratory pathology of the hog. Experimental results when compared to findings in the field indicate that the severity of the disease is linked to associated etiological factors as well as to the virulence of the viral strains themselves.

Animals↗
PubMed1983

[Epizootic swine influenza on animal farms in Brittany].

Since 1976, seroepidemiological surveys using radial haemolysis and isolation trials from samples collected from pigs at the abattoir showed that Brittany was an important reservoir of swine subclinical infections due to the human Influenza A viruses H3N2. Influenza A H1Sw N1Sw subclinical infections sporadically occurred from 1979 to 1981. In January 1982 an epizootic started in Finistère which spread from west to east. The H1Sw and N1Sw antigens of 28 stains isolated from 1979 to 1982 were studied with ferret and rabbit anti sera and appeared to be variants of the previous strains (1976-1967-1961-1930). A reinforced serological survey in pigs demonstrated that both Influenza A viruses H1Sw N1Sw and H3N2 simultaneously spread in herds. There is no actual prove of recent human infections by A H1Sw N1Sw virus.

Adolescent↗
PubMed1983

[Rubella and congenital cataracts].

Between April 3, 1977 and June 6, 1980, 97 children with congenital cataracts and 97 evenly matched control children were examined in order to clarify the biological criteria of congenital rubella and to estimate its importance in the etiology of congenital cataracts. Although the age limit had been fixed at 60 months, the 1978 rubella outbreak accelerated the immunization in young children and resulted in difficulties in interpreting some results. All children presenting with congenital cataracts associated with clinical symptoms of rubella embryopathy displayed anti-rubella antibodies including anti-rubella IgM up to the 13th month: they represent 16% of cases with congenital cataracts in this series. The rubella etiology could not be proven in children with clinically isolated congenital cataracts.

Antibodies, Viral↗
PubMed1982

Echovirus 11 dense particles: isolation and preliminary characterization.

Dense particles (density 1.44 g/ml) were isolated during purification in CsCl of echovirus 11 produced in HeLa cells. The dense particles had similar antigenic properties and similar RNA and protein composition to standard (density 1.33 g/ml) echovirus 11 particles. They differed from standard particles in their higher buoyant density, lower infectivity and slightly smaller diameter. In contrast to other picornavirus dense particles, echovirus 11 dense particles were present as a major component of virus population. This high ratio of dense particles was found only in virus from HeLa cells which produced non-haemagglutinating echovirus 11. Haemagglutinating echovirus 11 from primary monkey kidney (MK) cells did not contain any detectable levels of dense particles.

Centrifugation, Density Gradient↗
PubMed1982

Detection of virus-specific IgA antibodies in serum of kidney transplant patients with recurrent cytomegalovirus infection by enzymeimmuno and radioimmunoassay techniques.

The feasibility of using human cytomegalovirus (CMV)-specific IgA antibody determinations as a signal for early detection of recurrent CMV infections in eight renal transplant recipients was analyzed. Solid phase radioimmunoassay (RIA), enzyme-liked immunosorbent assay (ELISA) and immunoperoxidase assay (IPA) techniques were used for IgA antibody determinations. In parallel, IgG antibodies to CMV were studied by immunoperoxidase assay. A significant rise of CMV-specific IgG antibody titre was observed in all of these patients between 5 and 53 weeks post-transplantation. CMV-specific IgA antibody production was detected close to the time a rise in CMV IgG antibody was observed in seven out of eight patients studied by RIA and ELISA, and in six out of eight patients studied by IPA. In two patients specific CMV IgA antibodies were detected by all three methods before a significant rise of CMV IgG antibody titre was demonstrated. In these patients CMV IgA was detected by RIA earlier than by ELISA and IPA. The potential application of CMV-specific IgA antibody determination for early detection of recurrent CMV infection in renal transplant patients is discussed.

Adult↗
PubMed1982

Viral pollution of seawater at Barcelona.

A virological study of sea water was carried out at Barcelona (Spain) in June and September 1979. Three beaches were sampled in June; the September samples were taken in front of an outlet at 2, 5, 20 and 1,000 m offshore. All of the samples from the 3 beaches contained viruses, at rates varying from 0.12 to 1.72 MPNCU/l. Vaccinal poliovirus type 3 and coxsackie B5 predominated. For the offshore samples, 55% were positive with rates ranging from 0.15 to 0.36 MPNCU/l. Vaccinal poliovirus type 3 was clearly predominant. There was no significant difference in viral pollution rates between 2m and 1,000 m offshore.

Enterovirus B, Human↗
PubMed1981

Detection of specific IgA antibodies in serum of kidney transplant patients with recurrent cytomegalovirus infection.

59 sera of 10 immunosuppressed renal allograft recipients who experienced recurrent cytomegalovirus (CMV) infection were analyzed by enzyme-linked immunosorbent assay (ELISA) for CMV IgA antibodies and by the complement-fixation (CF) test. A significant rise of CF titer was evident 4-53 weeks post-transplantation. 9 patients produced CMV IgA in high titers at about the time of CF antibody rise was observed. 1 patient did not produce IgA antibodies to CMV. In 3 of the 9 patients, specific CMV IgA antibodies were detected before the rise if CF titer was demonstrated. CMV IgA antibodies were found to persist for as long as 6 weeks post-transplantation. The potential application of ELISA detection of CMV-specific IgA antibodies as an early indication of CMV infection in kidney transplant patients is discussed.

Adult↗