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Biomedical subjects

M Aslam

Publications and source records attributed to M Aslam.

At least 163 records · Page 9Linked to original sources

Reevaluation of anaerobic nitrite production as an index for the measurement of metabolic pool of nitrate.

The use of anaerobic nitrite production as an index for the measurement of metabolic pool of nitrate was reevaluated using primary leaves of 7-day-old barley and 10-day-old soybean seedlings. The seedlings were grown in nutrient solutions containing 5 to 15 millimolar nitrate. The nitrate-free in vivo assay system of nitrate reductase was used for measuring the production of nitrite. Both the duration and extent of nitrite production were dependent on the level of endogenous nitrate in the tissue. At cessation of nitrite production, 30 to 50% of the endogenous nitrate was reduced to nitrite. Nitrate from the tissue leaked continuously into the surrounding medium so that, at cessation of nitrite production, nitrate supply from the tissue was exhausted. The cessation of nitrite production, therefore, may have been caused by the depletion of endogenous nitrate from the tissue. It is concluded that anaerobic nitrite production is not a valid index for the measurement of the size of the metabolic pool of nitrate.

Journal Article↗

Sartan (cancer) and its treatment in Unani Medicine.

The knowledge of Sartan (cancer) in the Unani (Greco-Arabian) systems of medicine can be traced to ancient times (131-200 A.D.). Like all other diseases its etiology has also been attributed to the combustion and imbalance of certain humors in the body. Unani simples-plants, animals and minerals-and formulations used for the treatment of the disease have been tabulated with recipes, methods of preparation, doses (wherever available) and modes of administration. The claims have been reviewed in the light of research work conducted in India during the last three decades.

History, Ancient↗

Influence of light and ambient carbon dioxide concentration on nitrate assimilation by intact barley seedlings.

The influence of light, dark, and ambient CO(2) on nitrate assimilation in 8- to 9-day-old barley seedlings was studied. To develop the photosynthetic apparatus fully, the seedlings were grown in nitrogen-free Hoagland solution for 5 days in darkness followed by 3 days in continuous light.The seedlings reduced nitrate and nitrite in both light and dark, although more slowly in darkness. The slower nitrate reduction in darkness was not due to decreased uptake, since the steady-state internal concentration of nitrate was doubled. The faster nitrate reduction in light was attributed to recent products of photosynthetic CO(2) fixation supplying reducing energy, possibly by shuttle reactions between chloroplasts and cytoplasm. In carbohydrate-deficient tissue, it appeared that recently fixed photosynthate could supply all of the energy required for nitrate reduction. When sufficient metabolites were present in the green tissue, light was not obligatory for the reduction of nitrate and nitrite.

Journal Article↗

Surma and lead poisoning.

Blood lead concentrations were measured in 62 Asian children, of whom 37 had definitely had surma applied to their eyes and 25 were thought not to have done. The mean concentration in those who had not used surma was 0.98 +/- SD 0.42 mumol/1 (20.3 +/- 8.7 microgram/100 ml) compared with 1.65 +/- 0.68 mumol/4 (34.2 +/- 14.1 microgram/100 ml) in those who had. Analysis of 29 different samples of surma showed 23 of them to be composed largely of lead sulphide. We conclude that the use of surma is associated with high blood lead concentrations. In our cases most of it had been obtained abroad, and hence government restrictions might be ineffective in limiting its use: a better method of prevention might be to inform the leaders of Asian communities of the risks.

Asia↗

Effect of l-Canavanine on Nitrate Reductase in Corn Roots.

l-Canavanine inhibits the appearance of nitrate reductase (NADH-nitrate oxidoreductase, EC 1.6.6.1) in both root tips and mature root sections of corn (Zea mays L.). Ten-fold more canavanine was required to cause a 50% reduction in the level of nitrate reductase activity (NRA) in root tips than in mature root sections. For example with one particular batch of seeds 500 mum canavanine was effective in root tips whereas only 50 mum was required in mature root sections. In root tips arginine (1 mm) completely reversed the effect of 1 mm canavanine. In mature root sections higher concentrations of arginine (approximately 5 mm) were required for a complete reversal of the canavanine effect. Additions of canavanine to roots after a period of 3 hours with 5 mm KNO(3) resulted in a loss of NRA. NO(3) (-) protected nitrate reductase from this inactivation in both root tip and mature root sections.

Journal Article↗