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Biomedical subjects

M Asano

Publications and source records attributed to M Asano.

At least 541 records · Page 30Linked to original sources
PubMed1981

A new enzymatic method for the demonstration of choline in human seminal stains.

A new, simple method for the identification of seminal stains based on the reaction of choline oxidase with choline is presented. The key reaction is the production of hydrogen peroxide by the oxidase action on choline, whereby hydrogen peroxide reacts with N-ethyl-N-(3-methylphenyl)-N'-acetylethylenediamine and 4-aminoantipyrine (ampyrone) in the presence of peroxidase to produce a purple color so that seminal fluid can be identified. Some data are presented on the sensitivity, stability, and specificity of the present method.

Alcohol Oxidoreductases↗
PubMed1981

Differences in predominant energy substrate in relation to the resected hepatic mass in the phase immediately after hepatectomy.

Changes in the energy substrate utilized by the remnant liver after hepatectomy were studied in relation to the hepatic energy status in 25%, 70%, and 93% hepatectomized rabbits. In 25% hepatectomized rabbits, the energy charge ((ATP + 1/2ADP)/(ATP + ADP + AMP)) level of the remnant liver remained unchanged after hepatectomy and was not affected by portal infusion of (+)-octanoylcarnitine, an inhibitor of fatty acid oxidation. In contrast, the energy charge level of the remnant liver decreased rapidly after 70% hepatectomy and reached the lowest level of 0.77 at 12 to 24 hr after hepatectomy (p less than 0.01). At this time, the energy charge level was further decreased to 0.45 by (+)-octanoylcarnitine (p less than 0.001). It returned to near normal 4 days later. At this time, the effect of (+)-octanoylcarnitine was less evident, with the restoration of the energy change. In 93% hepatectomized rabbits, the energy charge level of the remnant liver decreased rapidly and steeply in the phase immediately after hepatectomy. It is suggested that the remnant liver metabolism switches to predominant utilization of fatty acid as an energy source when the energy charge decreases; it then becomes able to utilize glucose with the restoration of energy charge level. In addition, it is emphasized that hyperosmolar glucose solution or insulin administration during the early period after major hepatectomy should be reconsidered because of their inhibition of the mobilization of free fatty acid from adipose tissue.

Animals↗
PubMed1980

Immobilization of enzymes for medical uses on plastic surfaces by radiation-induced polymerization at low temperatures.

The immobilization of some medically useful enzymes were studied by means of radiation-induced polymerization at -78 degrees C. Glucose oxidase and glucose peroxidase were immobilized in the form of thin membranes inside polyvinyl chloride tubes and on polyethylene films; these membranes showed considerable activity yield, as well as good activity retention. Two effective methods were adopted to improve the surface properties of the base materials and to facilitate firm immobilization by coating: that is, an undercoating method followed by radiation curing of the undercoating and an irradiation grafting method with a monomer. Both were tested with good results. An immobilization of urokinase was also carried out successfully by similar methods. The thrombogenicity of the immobilized urokinase showed a remarkable effect on thombus formation.

Adhesiveness↗
PubMed1980

gamma-Glutamyltransferase test as a new tool for identification of seminal stains.

A simple qualitative method for identification of seminal stains based on a high activity of gamma-glutamyltransferase (gamma-GTP) in human semen is described. It employs the release of alpha-naphthylamine from N-gamma-glutamyl-alpha-naphthylamide by the gamma-GTP action: alpha-naphthylamine couples with Fast Garnet GBC salt to produce a strong brownish-red color. The data on its simplicity, specificity, and stability show that the present method is suitable for medicolegal examination of seminal stains as a preliminary test.

Adolescent↗
PubMed1980

General formulas of the estimated likelihood ratio Y/X in the diagnosis of paternity of a deceased putative father.

When the putative father is dead his probable genotype, essential for estimate of likelihood ratio in the diagnosis of paternity, should be deduced from his relatives. In the present paper are described the general method for such deduction of probable genotype and the derivation of the formula of likelihood ratio. 10(4) examples of the diagnosis of paternity of deceased father are examined using a Monte Carlo method and the distributions of relative frequencies of log(Y/X) are calculated for the true father and non-father. These results indicate that the present method of estimation of Y/X from relatives is quite useful for the diagnosis of paternity of a deceased putative father.

Blood Group Antigens↗
PubMed1980

Diagnosis of paternity for cases without mother and without both mother and putative father based on blood group findings from the relatives.

Formulas of the estimated likelihood ratio Y/X are derived for cases without mother as well as those without both mother and putative father, by using blood group findings of their relatives. The distribution curves of the relative frequencies of log(Y/X) for these cases are calculated with respect to 10(4) families which are created by a Monte Carlo simulation. The extent of success in the paternity diagnosis is clarified by the statistical analysis based on these distribution curves. According to the above analysis, fairly high chance of success can be obtained in the diagnosis of such ambiguous cases without the plaintive mother and/or the putative father, if there relatives are alive. It is also concluded that the genetic information as to the parents of the deceased person increases the exclusion probability, whereas that as to the spouse and children increases the fraction of log(Y/X) greater than 1 for non-father, corresponding to the fraction where the Essen-Möller value is less than 9%.

Blood Group Antigens↗