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Biomedical subjects

M Arya

Publications and source records attributed to M Arya.

At least 91 records · Page 5Linked to original sources
PubMed1975

The effect of chronically administered cannabis extract on the female genital tract of mice and rats.

Daily administration of cannabis extract (mice: 1 mg/day, rats: 5 mg/day for a period of 64 days) results in a cessation of ovarian cyclic activity as judged by vaginal smear and the absence of corpora-lutea in the ovaries of treated rats and mice. Distinct effects are produced upon uterine tissue. Uterine glands were regressed and the stromal edema was conspicuous. Cannabis extract resulted in a decreased uterine RNA, glycogen, and sialic acid concentration. It also brings about a significant depletion in the level of adrenal ascorbic acid.

Adrenal Glands↗
PubMed1975

The effects of chemical occlusion of vas deferens on the testicular function of dog: a preliminary study.

A new approach to rapid male sterilization was studies by giving a single injection of sclerosing chemical agents (10% silvernitrate, 3.6% Formaldehyde in ehanol, 5% potassium permagnate, 100% ethanol, and 3.6% Formaldehyde) directly into the vas deferens to determine whether blocking the vas has any adverse effects on the testicular function. 2 - Chemical occlusion of vas is quite effective in producing a block in the vas deferens of dogs. 3 - Ethanol injection in the vas deferens caused an atrophy of the testis. Extensive necrossis and exfoliation of the seminiferous elements were conspicuous. These changes increased in severity in 3.6% Formaldehyde in ethanol, 5% potassium permagnate, and 10% silver nitrate solution injected dogs. 4 - Decrease in the testicular contents of RNA, Protein and sialic acid, four weeks after vas occlusion were associated with degenerative changes of the spermatogenic elements. 5 - Testicular cholesterol and total lipids were increased following vas occlusion. 6 - Reduced sialic acid levels in the testis of vas occluded dogs indicates an inhibition of androgen production, which is further reflected in reduced leydig cell function. 7 - The role of vas deferens in determining the fate of a given testis is being discussed.

Animals↗
PubMed1975

Biochemical and histological changes in testes and epididymis of dog after vasectomy.

1. Changes in the histological structure and biochemical composition have been studied in the testes and epididymis of dog after unilateral and bilateral vasectomies. 2. Testes and epididymis weight did not change after 30 days of unilateral vasectomy. Whereas a significant decrease was observed after 60 and 120 days of unilateral and bilateral vasectomy. Tests and epididymis weights returned to normal after 180 days of bilateral vasectomy. 3. Histologically testes and epididymis were normal after 30 days of vasectomy. After 60 and 120 days of unilateral and bilateral vasectomy. Testes and generative changes. Seminiferous tubule and Leydig cell nuclear diameter reduced. Sixty to seventy percent tubules recover after 180 days of bilateral vasectomy. 4. Decrease in RNA, protein, sialic acid contents and increase in alkaline phosphatase activity, total lipids, cholesterol coincided with the absence of spermatozoa and atrophic changes in the testes. 5. The early degenerative changes were caused due to back pressure of testicular fluid (Vare et al, 1973).

Alkaline Phosphatase↗
PubMed1975

The effect of ligation of cauda epididymidis and vasectomy on testicular function in the adult gerbil (Meriones hurrianae).

1. The effects of ligation of caput, cauda epididymides and vasectomy were studied in adult gerbils. 2. The operations were performed unilaterally, the testis and epididymides on the contralateral side serving as controls. 3. Ligation of cauda epididymides decrease testicular weight, whereas caput ligation did not change the testis weight. Accessory sex glands were reduced in size. 4. Ligation caused a drastic degeneration of the spermatogenic cells. There was a complete disruption of testicular function. Leydig cell hypertrophy was conspicuous. Caput and cauda ligation led to degenerative changes in the epididymides. Epididymal epithelium was regressed and the lumen was devoid of spermatozoa. 5. Caput and cauda ligation inhibited the synthesis of RNA, protein and sialic acid in testis and epididymides and depleted the fructose concentration in the seminal vesicle. 6. Vasectomy did not cause any alteration in sperm production during the 8 week period on the lighted side. The cytology and the biochemistry of the testis and epididymides appeared to be normal.

Age Factors↗
PubMed1975

Reversible changes in the testes and epididymides of dog treated with alpha-chlorohydrin.

1. Chronic administration of alpha-chlorohydrin (8 mg/kg body wt for 30 days) caused lesions in the testis of dog. The changes in the germ cells were degenerative. The seminiferous tubule and Leydig cell nuclear diameter were reduced. 2. Epididymal cell height was greatly reduced and the stereocilia had disappeared completely. The lumen was devoid of spermatozoa. 3. Alpha-chlorohydrin administration inhibited the RNA and sialic acid contents in the testes and epididymides of dog. Total cholesterol and lipids/g of testes were increased significantly after alpha-chlorohydrin administration. 4. These effects were reversible. Repopulation of testis tubules occurred following a period of 100 days recovery in dog. Numerous spermatogonia and sperm develop and traverse the epididymides. The RNA, sialic acid, cholesterol and total lipids of testes and epididymides returned to subnormal levels. 5. The possibility of using alpha-chlorohydrin as male contraception is indicated.

Contraceptive Agents, Male↗
PubMedDate not supplied

Lectin-binding pattern of bull testis and epididymis.

Seven rhodamine-conjugated lectins (PNA, RCA I, SBA, Con A, WGA, UEA I, DBA) were used to study the distribution of glycoproteins in the testis and epididymis of immature, juvenile, and adult bulls. A marked change was found in the staining pattern of the lectins in the seminiferous tubules during acrosomal development, and the Sertoli cells seemed to have a cyclic affinity for some of the lectins. The distribution of lectin staining in six regions of the bull epididymis showed some typical differences that were associated with the secretory and absorptive functions of the organ. Region 1 was characterized by strong surface and villous staining and a patchy reaction in the principal cells. Regions 2 and 3 showed a strongly reactive apical Golgi zone and secretory material. In regions 4 and 5, the Golgi zone was subapical but strongly reactive with most lectins, while in region 6 a weakly reactive apical Golgi zone was found. During sexual maturation, an increasing number of basal cells with a strong affinity for some lectins was found at the periphery of the epithelium in regions 2 to 6. These regions also had lectin-stained material along the basal border of the principal cells. These findings suggest that the basal cells may be active in the digestion of absorbed material and that they derive from the principal cells, which may be active in transporting absorbed material to them. The staining pattern of the spermatozoa changed during their transit through the epididymis. The degenerating cells in the testis and epididymal tubules also showed an altered affinity for the lectins.

Acrosome↗
PubMedDate not supplied

A 3D medical image database management system.

We describe the design and implementation of QBISM (Query By Interactive, Spatial Multimedia), a prototype for querying and visualizing 3D spatial data. Our medical image application is focused on the brain mapping requirements for multimodality relationships across multiple subjects. It incorporates data describing both structure and function. It includes data structures that describe anatomy, physiology, coordinates using rendered imagery and statistical output. The system is built on top of the Starburst DBMS extended to handle spatial data types, specially, scalar fields and arbitrary regions or space within such fields. In this paper we list the requirements of the application, discuss the logical and physical database design issues, and present timing results from our prototype. We observed that the DBMS' early spatial filtering results in significant performance savings because the system response time is dominated by the amount of data retrieved, transmitted, and rendered.

Brain Mapping↗
PubMedDate not supplied

Distribution of lectin binding in rat testis and epididymis.

Seven lectins (PNA, RCA I, SBA, Con A, WGA, UEA I, DBA) conjugated with rhodamine were employed to analyse the staining pattern of glycoproteins with varying sugar residues in the testis and epididymis of adult Wistar rats. Some lectins (UEA I, SBA, DBA) gave rather specific staining of the mature acrosome, while others (PNA, RCA I) showed affinity for the early stages of acrosome formation or had a wide affinity for germinal and non-germinal cells and structures (Con A, WGA). In the epididymis the sperm mass had a homogeneous staining reaction with some lectins (PNA, RCA I, Con A, WGA, DBA) which also showed a rather strong reaction on the epithelial surface. It was concluded that this reaction is at least partially due to the secretory products synthetized by principal, apical, narrow and light cells of the epididymal epithelium. Some differences in the staining pattern of these cells were recorded indicating specialization of the cells for the production of distinct glycoproteins. The staining pattern of the interstitial and intertubular compartment of the testis and epididymis was also recorded.

Animals↗
PubMedDate not supplied

Lectin staining of rat testis and epididymis: effect of cyproterone acetate and testosterone.

Control and castrated (C) adult rats were treated with cyproterone acetate (CA), testosterone (T) or their combination (CA + T) for 15 days and the affinity of the testicular and epididymal tissue for seven rhodamine-conjugated lectins was analysed in fluorescence microscopy. In the testis CA caused the appearance of degenerating cells in the basal part of the seminiferous epithelium. These cells (spermatogonia, early spermatocytes) appear to be the most vulnerable germinal cells to anti-androgen treatment and this effect could be prevented by simultaneous administration of T. Castration caused a marked reduction in lectin staining of principal and light cells of distal caput (Dcp) and distal cauda (Dcd) epididymidis accompanied by the disappearance of the intratubular sperm mass. In castrated animals CA caused a moderate and T as well as CA + T a marked increase in the stainability of these cells and the appearance of homogeneous secretory material in the tubules. This material gave moderate or strong affinity for most of the lectins. Some minor differences were found in the staining pattern of the cells and the secretory material in Dcp and Dcd as well as after different treatments. This is consistent with local and qualitative differences in the epididymal secretory and absorptive activity, which can be further modified by the hormonal milieu.

Animals↗
PubMedDate not supplied

Effect of castration on lectin staining in rat epididymis.

Seven rhodamine-conjugated lectins (PNA, RCA I, Con A, WGA, UEA I, SBA, DBA) were used to follow the staining pattern of the rat epididymis at different time points after castration. The affinity of the intratubular sperm mass for the lectins increased rapidly with concurrent augmentation of the staining in the principal cells but a decline of the reaction in the light cells. The light cells showed some differences in their response to castration, which was compatible with secretory/absorptive activity in caput and absorptive activity in cauda. The active phase of sperm mass destruction and epithelial involution was accompanied by local accumulation of macrophages and round cells, which also acquired an increased affinity for most of the lectins. It is concluded that the androgen-deprived epididymis is rapidly programmed for autolytic and phagocytic processes, which include the destruction of macromolecules including glycoproteins of the spermatozoa.

Animals↗