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Biomedical subjects

M Arndt

Publications and source records attributed to M Arndt.

At least 19 recordsLinked to original sources
PubMed1998

Inhibition of alanyl aminopeptidase induces MAP-kinase p42/ERK2 in the human T cell line KARPAS-299.

Inhibition of alanyl aminopeptidase (EC 3.4.11.2, aminopeptidase N, CD13) expression, or activity compromise cell proliferation in a number of cell systems [1, 2, 3, 4, 5, 6]. The underlying mechanisms and the molecular components involved have not been identified as yet. In this study we show that inhibition of alanyl aminopeptidase enzymatic activity decreases the proliferation rate of the CD13-positive T cell line Karpas-299. By using the ATLAS cDNA expression array (Clontech) we identified the p42/ERK2 MAP kinase as one downstream target of probestin, a potent inhibitor of alanyl aminopeptidase. Probestin and another specific aminopeptidase inhibitor, actinonin, in addition to their capability of inducing erk-2 mRNA levels, significantly increase p42 phosphorylation state. This is the first report on signal transduction components possibly mediating the growth-modulatory effects of alanyl aminopeptidase inhibitors.

CD13 Antigens↗
PubMed1998

Reengineering: deja vu all over again.

There is growing interest among hospitals in reengineering. It promises dramatic improvements in performance: Costs will be reduced while work processes, productivity, and patient care will all improve. A review of the health care literature on reengineering shows that little evidence exists to support its claims. This article critiques the existing literature on reengineering and addresses the conundrum hospital executives encounter when faced with the decision to adopt a new management technique--such as reengineering--in the absence of proof of its efficacy.

Cost-Benefit Analysis↗
PubMed1998

[Sore throat after use of the laryngeal mask and intubation].

The laryngeal mask was developed by Brain in 1981 and described for the first time in 1983. It has been applied a few million times worldwide since 1988. One of the main complication is soreness of the throat. Two hundred unselected patients who had gynaecological procedures under general anaesthesia--100 under laryngeal mask airway and 100 under intubation--were surveyed to ascertain the incidence of sore throats under laryngeal mask airway. Each patient was asked about soreness of the throat during the next two days. The incidence and duration of sore throats were recorded using a pain scale (0-100). After narcoses with the laryngeal mask, 63 patients had no complaints. Thirty-seven had sore throats with an average pain score of 8.1 +/- 13.8 on the day of operation. The average pain scores showed a decreasing tendency (4.2 +/- 9.5 and 0.4 +/- 2.6) for the first and second postoperative day. After intubation narcoses, we recorded sore throats significantly more frequently (p < 0.05) and the average pain score on the day of operation was significantly higher (13.2 +/- 17.5; p < 0.05) than after narcoses with the laryngeal mask. Also after narcoses with the laryngeal mask, the average pain scores decreased quickly on the first and second postoperative days (5.1 +/- 9.0 and 0.2 +/- 1.4). There were no differences regarding sore throats between narcoses with the laryngeal mask or intubation on these two postoperative days. Using both anaesthesiological methods, sore throat was not related to duration of anaesthesia or the experience of the anaesthetist.

Adolescent↗
PubMed1998

Influences on nursing care volume.

This study explores influences on nursing care volume per case in four DRGs and five hospitals. After accounting for the effects of length of stay, patient characteristics, and severity of illness, the direct effect of factors describing the nursing staff and hospital was assessed. Skill mix and standing orders each were significant in one or more DRGs, but there was no significant association between the volume of nursing care and occupancy or staff availability. The management and research implications of the findings are discussed.

Adolescent↗
PubMed1997

The activation-dependent induction of APN-(CD13) in T-cells is controlled at different levels of gene expression.

Recently, it was shown that aminopeptidase N (E.C. 3.4.11.2, CD13) is up-regulated during mitogenic stimulation of peripheral T-cells. In this study, we demonstrate that the half-life of APN mRNA was considerably prolonged in these cells leading to a 2.7-fold increase of APN transcript level. The apparent half-life time of the APN transcript was investigated by the RNA synthesis inhibitor-chase method using actinomycin D. The steady-state APN mRNA levels was determined by a competitive RT-PCR. The half-lives estimated in resting T-cells, natural killer cells and permanently growing tumour cells varied between 3.5 and 6 h. Finally, nuclear run-on assays revealed that the APN gene expression of stimulated T-cells is controlled by increased promoter activity as well. These studies suggest a control of APN gene expression at the post-transcriptional level in addition to promoter-mediated regulation.

Binding, Competitive↗
PubMed1997

Rapid mitogen-induced aminopeptidase N surface expression in human T cells is dominated by mechanisms independent of de novo protein biosynthesis.

The membrane bound metalloprotease aminopeptidase N (APN, CD13, EC 3.4.11.2) is a well established marker of normal and malignant cells of the myelo-monocytic lineage. It is also expressed by leukaemic blasts of a small group of patients suffering from acute or chronic lymphoid leukaemia. Recently, the expression of the APN gene in T cell lines as well as the induction of APN gene and surface expression in human peripheral T cells by mitogenic activation have been demonstrated. Here, by means of cytofluorimetric analysis evidence is provided, that the induction of APN surface expression is partially resistent to the action of the inhibitors of protein biosynthesis, puromycin and cycloheximide, and is not prevented by tunicamycin, an inhibitor of glycosylation. These data suggest that the rapid mitogen-induced surface expression of APN, detectable 20 hours after stimulation is dominated by mechanisms not dependent on de novo protein biosynthesis or glycosylation. As shown by simultaneous analyses, the inhibitors used did also differently modify the induction of surface expression of other inducible glycosylated leukocyte surface antigens, namely CD25, CD69 and CD95.

CD13 Antigens↗
PubMed1997

[Legal boundaries of negative and positive lists].

Positive lists comprise only a limited number of medicines destined for reimbursement by the health insurance companies. Intentions to publicize those lists at federal and provincial levels failed for constitutional reasons. Negative lists--on the other hand--as prepared by health insurance companies are admissible as long as they are--among other things--open for changes according to the scientific progress. Medicines being incorporated into a negative list can be prescribed but are not reimbursable.

Drug Approval↗
PubMed1996

Benefits and disadvantages of corporate restructuring--the hospital view.

Many hospitals have adopted a corporate structure to improve management and gain economic benefits. Our study of Massachusetts hospitals found that for only a few had these anticipated advantages materialized, while many found that the new structure entailed added bureaucracy and other disadvantages. The findings suggest that hospitals adopt or keep the structure not only for possible economic reasons but also to sustain their legitimacy in the eyes of important stakeholders.

Annual Reports as Topic↗
PubMed1996

The interconnectedness of nurses' lives. Implications for nursing management.

OBJECTIVE: This study extends knowledge of how nurses' careers interact with their personal lives and professional development. BACKGROUND: Extant research suggests that nurses' career patterns are unstable or unplanned. However, these images may be a reflection of the models that are applied. Such models are overwhelmingly normative and do not reflect the actual life experience of nurses. METHODS: A series of interviews explored how nurses make changes in their lives. Questions focused on the stimulus for change and the resources used, problems associated with changes, and the effects of changes on the nurses themselves and others. RESULTS: The results show that nurses' careers, professional development, and personal lives are interconnected. CONCLUSIONS: The concept of interconnectedness reflects the confluence of events or people and their contemplation and integration by the nurses into their lives and relationships. The results contradict normative admonitions about career planning and have implications for the development of theories on nursing careers and for nurse managers.

Adaptation, Psychological↗