Search PubMed⌕ Search

Biomedical subjects

M Arnaud

Publications and source records attributed to M Arnaud.

At least 73 records · Page 4Linked to original sources
PubMed1987

[Osteochondromatosis of a distal interphalangeal articulation].

The authors report a case of an unusual site for chondromatosis in the hand: at the distal interphalangeal joint of the third ray. Swelling is the most important sign, without a history of trauma. A study of the literature revealed twelve publications of osteochondromatosis localised to the hand and only five at the interphalangeal joints of the fingers. The authors stress the necessity of a pathological examination and early surgery before joint destruction begins.

Aged↗
PubMed1987

Bacillus subtilis sucrose-specific enzyme II of the phosphotransferase system: expression in Escherichia coli and homology to enzymes II from enteric bacteria.

Sucrose is transported into Bacillus subtilis cells by way of a phosphotransferase system, which consists of a specific enzyme II, a nonspecific enzyme I, and a histidine-containing phosphocarrier protein. Mutations in the sacP locus abolish the specific transport of sucrose. The B. subtilis sacP gene was cloned and expressed in Escherichia coli, and transformed cells could transport and phosphorylate sucrose. This indicates that the sacP gene product is enzyme II of the sucrose phosphotransferase system of B. subtilis. The nucleotide sequence of the sacP gene was determined and was found to overlap with the sacA gene at the tetranucleotide ATGA, which may allow a translational coupling between sacP and sacA. The two genes are therefore probably organized in an operon structure with the promoter located 5' to sacP gene. The deduced amino acid sequence gave a Mr of 48,945 for the sucrose-specific enzyme II polypeptide. The amino acid sequence was compared to that of three other known enteric bacterial enzymes II (beta-glucoside-specific enzyme II, mannitol-specific enzyme II, and glucose-specific enzyme II). Homology was found with beta-glucoside enzyme II, and well conserved regions were identified through the comparison of the proteins.

Amino Acid Sequence↗
PubMed1987

The major acid-soluble proteins of Bacillus subtilis spores: partial amino acid sequence and forespore location of their mRNAs.

In Bacillus subtilis the alpha, beta, gamma and delta components comprise 80-90% of the total acid-soluble spore proteins (ASSPs). Sequence analysis demonstrates that alpha and beta share 32 of their first 36 amino acids and are closely related to the A and C ASSPs of Bacillus megaterium spores, confirming the results of analysis of their cloned genes. Despite the difference in apparent size of gamma and delta, they have identical N-terminal sequences (37 residues). Unless gamma and delta derive from very recently duplicated genes, it appears that gamma is derived from delta, either in vivo or during isolation. Although the sequenced regions of gamma and delta have no homology to alpha and beta, outside of the previously recognized pentapeptide recognition sequence for the spore endopeptidase, they share 10 and 15 residue peptides flanking this sequence with ASSP B of B. megaterium, but in reverse order. At least two groups of ASSPs have, therefore, been conserved between B. subtilis and B. megaterium: the multigene AC alpha beta family and the B gamma (delta) group. Sequence conservation in each group implies selection for functions in addition to storage. Both the alpha and beta components of B. subtilis ASSPs and their mRNAs are located in the forespore compartment of cells at t5.5 of sporulation, the time of most rapid ASSP synthesis. The sizes of these transcripts (250-350 bp) and their ability to direct the in vitro synthesis of ASSPs of mature size, indicate that genes for these ASSPs are monocistronic, consistent with dispersed map location. Synthesis of ASSPs is, therefore, coordinately controlled by selective transcription in the forespore.

Amino Acid Sequence↗
PubMed1987

[Cauda equina syndromes in ankylosing spondyloarthritis, detected urologically].

The authors stress the importance of sphincter disorders especially bladder, in these patients. These disorders may compromise the vital prognosis. An accurate neuro-urological work-up is absolutely necessary. It will guide the functional rehabilitation. Which, as a rule, will improve the patients' comfort and prevent the occurrence of urinary complications, with the use, as in the case reported, of non-sterile intermittent self-catheterization.

Aged↗
PubMed1986

[Stereotypy in infantile autism].

Using observations of the stereotyped productions in six cases of autism, the author has studied the different functions of the stereotypies. First of all the stereotypy is a containing envelope which protects by means of an archaic modality of motoric control, and filters exchanges with the exterior by the flexibility of its production. Moreover, the stereotypy is centered on selective sensations, bringing in to play body regions which are revealed in the first sensory experiences of the infant. This production of sensations is individualized by everyone in a particular and significant way of being in the world. Stereotypy, like an insatiable search for sensations never lost, never found, is the corporal pursuit of personal wholeness, always in question. Stereotypy, while curtailing the subject, can also be a generator because it is a personal anchor, an evocation of originating experience. It should in any case be understood as a possible mode of irreducible communication.

Autistic Disorder↗
PubMed1985

DNA methyltransferases in normal and avian sarcoma virus-transformed rat cells. Quantitation of 5-methyldeoxycytidine in DNA and enzyme kinetics study.

In rat kidney cells transformed by avian sarcoma virus (B77 strain) DNA is hypomethylated (2.61 +/- 0.07%) when compared to DNA extracted from normal cells (3.33 +/- 0.11%) as revealed by high-performance liquid chromatography analysis. Kinetics studies showed that no significant differences could be detected between DNA methyltransferase activities from normal and transformed cells with regard to apparent Vmax, apparent Km for S-adenosylmethionine (2.32 X 10(-6) M and 6.64 X 10(-6) M respectively) and apparent Ki for S-adenosylhomocysteine (9.2 X 10(-7) M and 7.8 X 10(-7) M respectively), when unmethylated duplex DNA was used as second substrate. Equivalent ratios of S-adenosylmethionine over S-adenosylhomocysteine were measured in each cell type and DNA methyltransferase activities from both sources were found to be strictly additive. These results show that the hypomethylation of DNA detected in transformed cells is related neither to alterations of enzymatic activities extracted from nuclei nor to unbalanced S-adenosylmethionine/S-adenosylhomocysteine ratios.

Animals↗
PubMed1984

Characterization of the precursor form of the exocellular levansucrase from Bacillus subtilis.

Expression of the cloned levansucrase gene (sacB) was demonstrated in E. coli minicells by assay of the enzyme in crude extracts, SDS-polyacrylamide gel electrophoresis and immunoblotting. The existence of a precursor form of the enzyme of MW 53000 was also demonstrated and confirmed by the DNA sequence corresponding to the NH2 terminal region of the protein.

Amino Acid Sequence↗
PubMed1984

[Therapeutic or palliative embolization aimed at analgesia for bone metastases of renal origin].

In addition to the chemotherapy, surgery and physical treatments usually employed in the treatment of bony secondaries from renal tumours, the authors propose embolization. They have performed this procedure in 4 patients, using a mixture of isobutyl-2-cyanoacrylate and lipiodol. Arteriography is performed before and after the embolization which is carried out under local anaesthesia. Disappearance or relief of pain was obtained in each case. This technique in no way changes the prognosis of the disease, but it can improve the quality of life of these patients. A review of the literature reveals that metastases other than renal have already been embolized, as have primary malignant bone tumours. The authors describe their method, the incidents associated with treatment, the contra-indications and the supposed mechanism of the analgesic action of embolization.

Aged↗
PubMed1984

[Trisomy 12(pter----q12) and monosomy 21(pter----q21). A propos of a case].

The authors report an observation of a child with both trisomy 12(pter----q12) and monosomy 21(pter----q21). It is thus possible to detect the clinical signs which can be attributed to trisomy 12p and to monosomy 21ql respectively. The authors point out the originality of the maternal translocation which differs from the translocations affecting these two chromosomes previously described in the literature. Finally, the rarity of the type of adjacent-2 segregation is shown, and discussed according to the literature already published.

Chromosome Aberrations↗