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Biomedical subjects

M Appel

Publications and source records attributed to M Appel.

At least 73 records · Page 4Linked to original sources
PubMed1980

Effect of streptozotocin diabetes on sialic acid content and glycoprotein binding of isolated hepatocytes.

The possible correlation between sialic acid content of hepatocyte plasma membranes, and their binding capacity was investigated. For that purpose, [3H] asialotransferrin binding was tested with hepatocytes from both normal and streptozotocin treated rats. The observed decrease in binding capacity was parallel to a decrease in the sialic acid content Insulin therapy restored simultaneously normal blood glucose level, hepatic membrane sialic acid content and binding capacity.

Animals↗
PubMed1980

A comparison of measles and canine distemper virus polypeptides.

The polypeptides induced by canine distemper virus (CDV) strains have been characterized by polyacrylamide slab gel electrophoresis of infected cell lysates labelled with 35S-methionine, 14C-amino acids, 3H-glucosamine and 3H-mannose, or 32P-orthophosphate. Seven virus-induced polypeptides have been asigned the following nomenclature and mol. wt.: a large polypeptide L (180,000); a large glycoprotein G (77,000); a nucleocapsid-associated protein P (73,000); the nucleocapsid protein N (60,000); the smaller glycoprotein F0 (59,000); a membrane protein M (35,000) and a small polypeptide S (15,000). During pulse-chase experiments with 3H-glucosamine and 14C-amino acids the intensity of the F0 band decreases and that of the F1 and F2 bands increases; the H polypeptide band becomes more diffuse and the S-protein disappears. The N- and P- but not the M-proteins have been found to be phosphorylated. The polypeptide pattern of the Onderstepoort strain of CDV has been compared with that of two other CDV and with 17 measles and subactue sclerosing panencephalitis (SSPE) strains. Differences in the mobilities of various polypeptides have been observed between CDV and measles and SSPE strains; however, the only consistent difference is the mol. wt. of the M-protein of CDV strains which is smaller by 2000 than that of MV and this may be a biochemical marker to distinguish CDV from measles and SSPE virus strains.

Animals↗
PubMed1978

Maintenance of 2,3-DPG and ATP levels in blood stored for 30 days at a constant pH obtained by variation of CO2.

A novel procedure for storing blood at a controlled pH consists in collecting blood in a pH 8.20, Tris-CPD solution and storing it in a special recipient including a gas permeable membrane under a CO2 atmosphere. The recipient is placed in an atmosphere of variable CO2 content, so that the initial alkalinity of the preservative is balanced by dissolved CO2, the proportion of which is diminished when lactate production increases with storage. 2,3-DPG and ATP were studied at three different pH levels of approximately 7.25, 7.45, and 7.65 at 4 degrees C. The best pH for the simultaneous maintenance of 2,3-DPG and ATP was 7.65. Under these conditions, 2,3-DPG is maintained at its initial level and ATP at 55% of its initial level at the 30th day. Lactate production is linear and hemolysis moderate.

Adenosine Triphosphate↗
PubMed1978

The canine contagious respiratory disease complex (kennel cough).

Several infectious agents are involved in the kennel cough complex in dogs. They include canine parainfluenza virus (SV5), canine adenovirus 2, Bordetella bronchiseptica, and possibly several mycoplasma species. The importance of each of these agents in the disease syndrome is discussed as well as possible prevention or treatment.

Adenoviridae Infections↗
PubMed1976

Biochemical heterogenity of the left ventricular myocardium: transmural and base-apex gradients on H.E.P. contents of the well oxygenated perfused dog heart.

An investigation into the contents (mumol/g w.w.) of the creatine phosphate (CP) and adenosine triphosphate (ATP) of various myocardial regions was performed on the empty beating isolated blood perfused dog heart. Across the myocardial wall, CP concentration was higher in the outer portion (9.03 +/- 0.18) than in the inner portion (8.59 +/- 0.20) (P less than 0.05). In addition to this transmural gradient, a longitudinal gradient was found: tissue contents of both CP and ATP decrease significantly from the base (CP: 9.70 +/- 0.28, ATP: 5.84 +/- 0.20) to the apex (CP: 8.69 +/- 0.27, ATP: 5.57 +/- 0.17) (P less than 0.01 and P less than 0.05 respectively). The gradients of metabolites in the well oxygenated empty beating heart suggest that a permanent metabolic adaptation to less adequate regional O2 reserves is physiologically operating in the subendocardial layer and the apex region of the left ventricle.

Adenosine Triphosphate↗
PubMed1975

Sporadic bovine meningo-encephalitis-isolation of a paramyxovirus.

Isolation of a viral agent (107) directly from brain explants of a 15-month-old heifer with symptoms of a sporadic encephalomyelitis is described. The virus shares properties with the paramyxovirus family. It grows in a variety of cell cultures from different species, and induces nuclear and cytoplasmic inclusion bodies in infected cells. Nucleocapsids measuring 17 nm in diameter were found in the nucleus and cytoplasm of these cells when studied electron microscopically, thus indicating a close relationship of the agent to the measles-distemper-rinderpest group. No infectious virus was released from infected cells, although alignment of nucleocapsids was observed beneath the cell membrane, and no hemagglutinating activity could be detected with the methods employed. The 107 agent was compared serologically with parainfluenza viruses type 1, 2 and 3, simian virus 5, mumps and Newcastle disease virus (NDV), two bovine respiratory syncytial viruses and measles/subacute sclerosing panencephalitis, distemper and rinderpest viruses, always using 107 virus infected CV1 cells and antiserum of the different viruses in indirect FA tests. Positive FA reactions were observed only with two sera obtained from SSPE patients with high antibody titer to SSPE virus, and with one rabbit-anti-rinderpest serum. The titers of these sera to 107 virus, however, were significantly lower than those against homologous viruses. Five out of 9 sera from randomly selected healthy cattle showed antibody titers between 1:10 and 1:80 to 107 virus in FA tests. The significance of these results is discussed with respect to the epidemiology of SSPE in children and its possible implication with rinderpest in Europe.

Animals↗
PubMed1975

Canine adenovirus type 2-induced immunity to two canine adenoviruses in pups with maternal antibody.

Twenty-four Beagle pups with high levels of maternal antibody to canine adenovirus type 1 (CAV-1) and canine adenovirus type 2 (CAV-2) were oronasally inoculated with CAV-2 at 4 weeks of age. The CAV-2 was isolated from pharyngeal swabs on postinoculation days 2 through 6. In spite of the infection, maternal antibody continued to decrease for 4 to 8 postinoculation weeks, and then homologous CAV-2 neutralizing antibody and, to a lesser extent, CAV-1 neutralizing antibody began to increase. When these pups were challenge inoculated with CAV-1 and CAV-2 at a time when maternal antibody to CAV-1 would normally have disappeared, they were immune. In addition, 3 pups with maternal antibody to CAV-1 and CAV-2 were intramuscularly inoculated with CAV-2 at 3 weeks of age. Virus was not isolated from these pups, and maternal antibody decreased at a normal rate. These pups were not immune to challenge inoculation with CAV-1 and CAV-2.

Adenoviridae↗
PubMed1965

A study of Toxoplasma infection in chickens and cats on a family farm.

Presumptive evidence of toxoplasmosis was obtained following histological and serological studies of tissues from chickens and cats kept on a family farm. This was confirmed by transmission of the infection to experimental laboratory animals and back to chickens. The significance of the low grade serological reaction in one of the persons living on the farm was not clear.

Animals↗
PubMed1965

Hog cholera. V. Demonstration of the antigen in swine tissues by the fluorescent antibody technique.

The fluorescent antibody technique was employed to detect hog cholera virus in tissue sections of various organs from experimentally infected swine. The method proved to be highly sensitive and infection could be detected in these animals as early as three days after inoculation with the virus. Best results were obtained when tissues were collected from young animals in advanced disease rather than from sows or from pigs in the early febrile phase. Tonsil, spleen and lymph node were the tissues of choice and were most satisfactory when removed from freshly killed animals rather than from those that had died.

Animals↗
PubMed1965

Hog cholera IV. Detection of the virus in tissue culture preparations by the fluorescent antibody technique.

The fluorescent-antibody technique was employed for detection of hog cholera virus in tissue cultures inoculated with spleens of infected animals. As controls, cultures were also inoculated with material from normal swine and from those infected with other agents. In the first series 71 of 73 infected spleens, or 97 per cent, were detected. There were no false positive reactions among the controls. Results obtained with the second series of pigs showed that spleens collected during advanced stages of the disease were more satisfactory specimens than those collected earlier during the high temperature phase of infection. Findings with the third series of older swine indicated that their spleens were less satisfactory as a source of virus than those from young pigs. Tissues from freshly killed animals provided better specimen material than those from animals which had died.

Animals↗