Stabilization of nucleic acids in whole blood: an alternative to Guthrie cards.
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Biomedical subjects
Publications and source records attributed to M Anhalt.
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The lux genes of Xenorhabdus luminescens, a symbiont of the nematode Heterorhabditis bacteriophora, were cloned and expressed in Escherichia coli. The expression of these genes in E. coli was qualitatively similar to their expression in X. luminescens. The organization of the genes is similar to that found in the marine luminous bacteria. Hybridization studies with the DNA that codes for the two subunits of luciferase revealed considerable homology among all of the strains of X. luminescens and with the DNA of other species of luminous bacteria, but none with the nonluminous Xenorhabdus species. Gross DNA alterations such as insertions, deletions, or inversions do not appear to be involved in the generation of dim variants known as secondary forms.
Massive diuresis after relief of urinary tract obstruction has been divided into 3 categories: 1) salt, 2) urea and 3) water. Measurements of the urinary electrolytes and urine osmolality establish the character of the diuresis and facilitate in the fluid management of these patients. A clinical example of the 2 most common varieties, salt and urea, is presented in conjuction with the method of fluid replacement.
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