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Biomedical subjects

M Ando

Publications and source records attributed to M Ando.

At least 1,135 records · Page 63Linked to original sources
PubMed1979

[A study of the obstacles to reduction in congenital dislocation of the hip (author's transl)].

Investigation on ten patients with typical congenital dislocation of the hip generalized joint laxity indicated the changes in the antero-inferior part of the acetabulum, which are mainly adhesion and narrowing produced by the capsule pulled superoposteriorly, as the major obstacle for successful reduction. Inverted limbus, pulvinar mass and ligamentum teres may represent secondary obstacles mostly due to iatrogenic procedures. The purpose of the closed reduction is to get rid of the obstacles during the conservative treatment. The cause of the failure of conservative treatment of congenital dislocation of the hip is a misjudgement of true reduction, allowing enough cavity for the femoral head in the antero-inferior part of the joint without the presence of the capsule between the femoral head and the acetabular cartilage. To get better understanding of the true reduction, tangential view in the arthrography of the hip joint gives us more precise data about the antero-inferior part of the joint because the acetabulum faces the joint cavity laterally and anteriorly.

Arthrodesis↗
PubMed1979

Formaldehyde dehydrogenase from Pseudomonas putida. Purification and some properties.

Formaldehyde dehydrogenase was isolated and purified in an overall yield of 12% from cell-free extract of Pseudomonas putida C-83 by chromatographies on columns of DEAE-cellulose, DEAE-Sephadex A-50, and hydroxyapatite. The purified enzyme was homogeneous as judged by disc gel electrophoresis and was most active at pH 7.8 using formaldehyde as a substrate. The enzyme was also active toward acetaldehyde, propionaldehyde, glyoxal, and pyruvaldehyde, though the reaction rates were low. The enzyme was NAD+-linked but did not require the external addition of glutathione, in contrast with the usual formaldehyde dehydrogenase from liver mitochondria, baker's yeast, and some bacteria. The enzyme was markedly inhibited by Ni2+, Pd2+, Hg2+, p-chloromercuribenzoate, and phenylmethanesulfonyl fluoride. The molecular weight of the enzyme was estimated to be 150,000 by the gel filtration method, and analysis by SDS-polyacrylamide gel electrophoresis indicated that the enzyme was composed of two subunit monomers. Kinetic analysis gave Km values of 67 microM for formaldehyde and 56 microM for NAD+, and suggested that the reaction proceeds by a "Ping-pong" mechanism. The enzyme catalyzed the oxidation of formaldehyde accompanied by the stoichiometric reduction of NAD+, but no reverse reaction was observed.

Aldehyde Oxidoreductases↗
PubMed1978

Transfer of 2,4,5,2',4',5'-hexachlorobiphenyl and 2,2-bis-(p-chlorophenyl),1,1,1-trichloroethane (p,p'-DDT) from maternal to newborn and suckling rats.

Pregnant rats were given a small dose of 14C-2,4,5,2',4',5'-hexachlorobiphenyl (HCB) and 3H-DDT intraperitoneally. The transfer of HCB and DDT through the placenta and milk was then investigated. Transfer through the placenta was 2.7 and 1.5% (respectively) of the initial doses; transfer through milk was 39.2 and 21.5%. HCB is obviously more transferable than DDT through the placenta and milk, the ratio of the amount of HCB transferred through milk to the amount transferred through the placenta agrees with that for DDT. Concentrations of HCB and DDT in the whole suckling rat increases rapidly and is similar to the sigmoidal growth curve and change in lipid concentration. Therefore, the concentrations of the chemicals in the maternal tissue generally decrease in comparison with those of nonpregnant rats.

Animal Population Groups↗
PubMed1978

Activation of alveolar macrophages exposed to lavage-procured immunoglobulin G obtained from normal rabbit lungs.

Pulmonary washings from rabbits were freed of cells and added to the monolayers of homologous alveolar macrophages (AM). At 1 h after incubation with the pulmonary washings, many more cells adhered to glass, spread out, and showed enhanced Nitro Blue Tetrazolium reduction. The maximal effect of the pulmonary washings on AM activation was obtained 12 h after incubation. The AM activated by the pulmonary washings showed a higher capacity to inhibit the growth of intracellular BCG, and that capacity was correlated with the intensity of Nitro Blue Tetrazolium reduction by the AM. Gel filtration of the pulmonary washings through Sepharose 4B yielded five fractions. The factor that activated the AM functions was in fraction 4. When the immunoglobulin G in the fraction was removed by an immunoadsorbent column, AM activity was abolished. The effect of the immunoglobulin G was dose dependent, and minimal responses to 10(6) cells per ml were obtained at a protein concentration of 20 mug/ml. Lymphokines had no effect on AM activation with respect to the morphological alterations and Nitro Blue Tetrazolium reduction during the 24-h observation time. In summary, AM from normal rabbits were soon activated markedly by lavage-procured immunoglobulin G, but not by lymphokines.

Animals↗
PubMed1978

Distal type of aortopulmonary window. Report of 4 cases.

We have studied 14 patients with aortopulmonary window (10 male and 4 female, age range 1 month to 41 years). Four of these had a distal defect with characteristic haemodynamic and angiographic features. Aortopulmonary window may be classified into 3 types: type I (proximal) defects occur in the proximal part of aortopulmonary septum; type II (distal) defects occur in the distal part of the aortopulmonary septum adjacent to the right pulmonary artery; the type III defect is a combination of types I and II. In type I, injection of contrast media into the aortic root opacifies the main pulmonary trunk and then both pulmonary arteries. In type II, the right pulmonary artery is preferentially opacified simulating the finding of right pulmonary artery arising from the ascending aorta. In one case of type II, injection into the right ventricle showed preferential flow to the left pulmonary artery, because of the large shunt of unopacified blood into the right pulmonary artery, but in both types I and II the left and right pulmonary arteries are usually opacified simultaneously after injection into the main pulmonary trunk. In type I either transaortic or transpulmonary closure is the appropriate surgical procedure. In types II and III, the transaortic approach provides better exposure and facilitates the operative repair.

Aorta, Thoracic↗
PubMed1978

The mode of inheritance in idiopathic cardiomyopathy, preliminary report.

Genetic analyses of 565 first-degree relatives of 99 patients with ICM suggests that ICM has both simple dominant and recessive modes of inheritance. There was no clinical form of ICM specific to the mode of inheritance, both hypertrophic and congestive types almost equally observed both modes of inheritance. Two pairs of identical twins who suggested the simple recessive mode were reported.

Adolescent↗