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Biomedical subjects

M Andersson

Publications and source records attributed to M Andersson.

At least 469 records · Page 26Linked to original sources
PubMed1984

Splenic macrophages during pregnancy in the mouse.

Pregnant CBA/Ca mice at mid-gestation showed a two-fold increase in the relative content of splenic macrophages, a change which was suggested to be due to enhanced migration. The proportion of spleen cells carrying a high density of Fc-receptors (assayed by EA-rosetting) was similarly increased in pregnant mice. The splenic content of non-lymphoid strongly esterase-positive cells was dramatically elevated at mid-gestation, but this increase was shown basically to be due to elevated numbers of esterase-staining erythroid blast cells. The pattern of the increase of Ig-secreting spleen cells was compared with that of macrophages and erythroid blast cells, and possible unspecific interaction at tissue-level is discussed.

Animals↗
PubMed1984

Genomic hybridization with class II transplantation antigen cDNA probes as a complementary technique in tissue typing.

Hybridizations of HLA-DR and DC transplantation antigen beta chain cDNA probes to restriction enzyme digested genomic DNA were examined in regard to their potential in tissue typing. DNA from cells, typed by cellular techniques to be homozygous for the specificities Dw 1 to 8, gives rise to unique fragment patterns for each specificity. Hybridizations to DNA from unrelated individuals serologically typed to be DR identical occasionally reveal genetic differences in the class II antigen region, notably with the DC beta cDNA probe. In contrast, hybridizations to DNA from monozygotic twins displayed completely identical patterns with both types of probes. From these data it seems reasonable to conclude that genomic hybridizations with class II antigen probes will be useful in the definition of new class II antigen loci and alleles, in determination of paternity, in studies of class II antigen linked diseases and as a complementary method in conventional tissue typing.

Alleles↗
PubMed1984

Isoelectric focusing followed by silver staining. A suitable method for routine investigation of cerebrospinal fluid proteins.

Cerebrospinal fluid (CSF) and serum samples from 361 consecutive patients were investigated. The protein pattern of each sample was examined by isoelectric focusing (IEF) of unconcentrated CSF followed by silver staining and by agar gel electrophoresis of concentrated CSF stained with amido black. 347 paired samples were compared. The results were the same in 83% of the samples. Discordant results were found in 10% of the samples. and in 7% additional information was achieved by one of the methods. IEF was superior to agar gel electrophoresis for the identification of oligoclonal immune reactions of the central nervous system. Tau globulin increase was seen in a few CSF samples, more often with IEF and then associated with other changes of the pattern. IEF of unconcentrated CSF followed by silver staining only requires 20 microliters CSF, while 3-4 ml CSF is needed for the agar gel electrophoretic method. It was concluded that IEF has several advantages compared to agar gel electrophoresis and is suitable as a routine method in clinical laboratories.

Adolescent↗
PubMed1984

Serum acute-phase glycoproteins and immune complexes in relation to smoking and respiratory symptoms: an epidemiological study.

A nephelometric assay of concanavalin A binding was used to measure serum acute-phase glycoproteins in young conscripts, in middle-aged males, and in adults with persistent respiratory symptoms. The findings in conscripts, irrespective of the smoking status, did not differ from those in blood donors, whereas, a strikingly higher portion (38%) of the middle-aged heavy smokers exhibited elevated values. In subjects with persistent respiratory symptoms the corresponding figure was even higher (52%), but no difference was recorded between smokers and non-smokers. Elevated amounts of circulating immune complexes, as measured by conglutinin-binding assay, were detected in 6% of the middle-aged males and in 20% of the subjects with persistent respiratory symptoms.

Acute-Phase Proteins↗
PubMed1983

Determination of plasmin-alpha 2-antiplasmin complex in plasma samples by means of a radioimmunoassay.

Immunization of a goat with partially reduced and S-carboxymethylated plasmin B-chain-alpha 2-antiplasmin complex resulted in a large population of antibodies with rather high specificity towards the complex. These antibodies do not react with plasminogen or plasmin in complex with other inhibitors than alpha 2-antiplasmin. However, they react fully with native alpha 2-antiplasmin, but a 200-fold higher concentration, as compared to plasmin-alpha 2-antiplasmin complex, is needed to obtain a similar displacement curve in a double-antibody radioimmunoassay. The results indicate a conformational change in the vicinity of the reactive site in alpha 2-antiplasmin, as a result of complex formation with plasmin. A method for determination of plasmin-alpha 2-antiplasmin complex in plasma has been elaborated using the described radioimmunoassay. About 1.5 mg plasmin-alpha 2-antiplasmin complex/l can be detected, which equals the condition when about 1% of the alpha 2-antiplasmin in plasma is in complex with plasmin. In normal individuals plasmin-alpha 2-antiplasmin complex could be detected only rarely. However, patient with acute processes, as evidenced by high fibrinogen levels, surgical patients postoperatively or patients with malignancy have often detectable levels.

Antifibrinolytic Agents↗
PubMed1983

Viral antibody screening system that uses a standardized single dilution immunoglobulin G enzyme immunoassay with multiple antigens.

We present an enzyme-linked immunosorbent assay (ELISA) system for the simultaneous determination of immunoglobulin G antibodies directed against several viruses. Antibodies to up to eight different viruses could be determined for three different sera on one microtitration plate. After subtraction of the absorbance values obtained with the control antigens, the viral antigen absorbancies were expressed as percentages of the absorbance obtained with a pooled immunoglobulin standard. This value, the relative antibody activity, was rapidly calculated by means of a computer directly connected to the ELISA photometer and was stored on magnetic disks, thereby facilitating seroepidemiological studies. The reproducibility of the relative antibody activity was calculated to at best +/- 3.6% (standard deviation) in an intraassay test and to at worst +/- 20.4% (standard deviation) in an interassay test. Each serum was analyzed only at a dilution of 1/75. The sensitivity of this single-dilution ELISA (SD-ELISA) method for the detection of titer rises was compared with those of conventional methods, mostly complement fixation but also hemagglutination inhibition. A total of 142 of 155 (92%) paired sera showing fourfold complement fixation or hemagglutination inhibition rises also showed significant results in SD-ELISA. A total of 22 of 57 (39%) significant relative antibody activity rises were significant in complement fixation or hemagglutination inhibition. Overall, up to twice as many significant titer rises could be detected with SD-ELISA. Most of these seemed to have a sound correlation with clinical data. The specificity of SD-ELISA was found to be similar to that of complement fixation, with some cross-reactions occurring between herpes simplex and varicella-zoster virus antigens and between parainfluenza viruses. We have found SD-ELISA to be a valuable clinical virological tool that supplements conventional serology.

Antibodies, Viral↗
PubMed1983

Central nervous system involvement in early syphilis. Part 1. Intrathecal immunoglobulin production.

The immunological activity in the cerebrospinal fluid (CSF) was studied in 47 patients with early syphilis. The immunoglobulin production within the central nervous system (CNS) was estimated by analysing immunoglobulin G and albumin in CSF and serum and constructing an IgG index according to Tibbling et al. and calculating daily CNS IgG synthesis, according to Tourtellotte. In 10 (21%) of the 47 patients, intrathecal immunoglobulin production was observed. The immunoglobulin production within the CNS was significantly correlated to the presence of syphilis antibodies and to pleocytosis. Eight of the patients demonstrated oligoclonal bands on electrophoresis. Total protein usually did not reveal an increased CNS immunoglobulin production but reflected a blood-brain barrier lesion. One year after penicillin treatment, 2 out of 9 patients still had slight intrathecal immunoglobulin production.

Adult↗
PubMed1983

Central nervous system involvement in early syphilis. Part II. Correlation between auditory brain stem responses (ABR) and cerebrospinal fluid abnormalities.

Twenty-six consecutive patients with secondary or early latent syphilis, all with normal neurological findings, were studied with auditory brainstem responses (ABR), cerebrospinal fluid (CSF) analysis and an assay for immune complexes in serum and CSF. Seven of the 26 patients studied had a pathological ABR pattern and 14 had one or more pathological CSF tests. Fifteen patients had circulating immune complexes in serum. No patient had demonstrable immune complexes in CSF. A significant correlation was found between an abnormal ABR pattern and pathological CSF findings--but not between pathological ABR and presence of immune complexes. ABR seems to be a sensitive method for detection of early asymptomatic CNS involvement in syphilis.

Adolescent↗
PubMed1983

Ectopic pregnancy in the liver. Report of a case and angiographic findings.

A 23-year-old woman underwent laparotomy due to physical signs of intra-abdominal bleeding. A 3 X 3 cm bleeding mass adherent to the liver surface was found. Microscopic examination of the removed encapsulated tumour demonstrated an ectopic pregnancy of the liver. Angiography performed on the 10th postoperative day showed a hypervascular lesion in the right liver lobe. The angiographic findings are similar to those previously described in cases of tubal pregnancies.

Adult↗
PubMed1982

Reversed-phase ion-pair chromatography of tetracycline, tetracycline analogs, and their potential impurities.

Methods are presented for the separation of tetracycline, tetracycline analogs, and their potential impurities by reversed-phase ion-pair chromatography. The mobile phase consisted of a phosphate buffer with tripropylamine or N,N-dimethyloctylamine as counterions and acetonitrile as the organic modifier. The chromatographic properties of the tetracyclines were significantly improved by addition of the tertiary amines. The best result was obtained with N,N-dimethyloctylamine as the counterion, which gave good separation efficiency and peak symmetry for most of the substances studies. Addition of the tertiary amines to the mobile phase also significantly affected the capacity factors of the tetracyclines. Stability studies of the tetracyclines showed a fast degradation of chlortetracycline to isochlortetracycline and of lymecycline to tetracycline in phosphate buffer solutions of different pH. The purity of pharmaceutical preparations of tetracyclines was also investigated.U

Acetonitriles↗
PubMed1981

Epidural morphine for postoperative pain relief.

Thirty-three patients were randomly assigned to two groups to study the analgesic potency, duration of action and side effects of epidural and intramuscular morphine after hip surgery. Two milligrams of preservative-free morphine chloride in 10 ml of normal saline in the epidural space was compared to 10 mg of intramuscularly administered morphine. There was a more rapid onset of action after intramuscular morphine. However, the quality of pain relief was substantially higher and the duration of action markedly longer after epidural morphine. The total dose required in the epidural group was 3.6 mg and in the intramuscular group 41 mg during the 15-h observation period. The side effects of epidural morphine were few and mild, the most embarrassing being urinary retention (20%). Nausea and/or vomiting was less common after epidural morphine (20% versus 55%). Pruritus or respiratory depression which have been reported previously were not encountered. However, it is recommended that preservative-free solution are used to avoid itching and that the patients are monitored, as respiratory depression may occur long after administration of epidural opiate.

Aged↗
PubMed1980

Longitudinal studies of anthropometric data and body composition. The population study of women in Götenberg, Sweden.

Anthropometric data and body composition, estimated from total body potassium and total body water determinations, were studied in 1968 to 1968 and 1974 to 1975 in a population sample of middle-aged women. Altogether 1302 women participated in the anthropometric studies and 79 women in the body composition studies on both occasions. Body height decreased with age both secularly and biologically. The reduction of body height was greater at higher ages. Body weight increased with age, the change being partly a cohort effect and partly an effect of age. Evidence of a reduced muscle mass in the arms with age was found. Body cell mass increased with age in some age strata. There was an increase in subcutaneous fat with age, both in the arms and in the trunk. Body fat did not change significantly with age in the age strata studied. A change in body weight was accompanied by a smiliar change in both body fat and body cell mass.

Adult↗