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Biomedical subjects

M Aiba

Publications and source records attributed to M Aiba.

At least 109 records · Page 6Linked to original sources
PubMed1984

Lymphatic system or hydro-oncotic forces. Which is more significant in drainage of pleural fluid?

Artificial pleural fluids, containing plasma protein from 0.1 to 9.0 g per 100 ml, indocyanine green (ICG) and para-aminohippurate (PAH), were injected into the pleural cavity of dogs. The concentration of these two indicators were measured at a regular time interval, and the lymphatic and non-lymphatic flows and PAH clearance were calculated by Stewart's and Takashina's equations. When the protein concentration in the pleural fluid was less than 4 g per 100 ml, the pleural fluid volume decreased at a rate of 0.20 ml/kg/hour. The decrease was due to pleural fluid drainage by the lymphatic flow, because the inward and outward non-lymphatic flows due to hydro-oncotic forces balanced each other and the net non-lymphatic flow was essentially zero. When the protein concentration was 6 g per 100 ml or more, the decrease of pleural fluid was remarkably attenuated, because of an increase of the inward non-lymphatic flow due to an increase of protein concentration in the pleural fluid. Even in these situations, the lymphatic flow was kept at the rate of 0.20 ml/kg/hour, similarly with the former conditions. From these results, it may be concluded that the lymphatic system is a principal factor, and that factors governed by hydro-oncotic elements have additive effect to that of the lymphatic system in the regulation of pleural fluid dynamics.

Animals↗
PubMed1981

Antibodies to Epstein-Barr virus-specific antigens in patients with hairy-cell leukemia.

Antibody titers to Epstein-Barr virus (EBV)-specific antigens of 19 patients with hairy-cell leukemia (HCL) were markedly elevated. All patients showed high titers of IgG anti-viral capsid antigen (VCA) reactivity equal to or greater than 320 (reciprocal titer). The anti-VCA reciprocal geometric mean titer (GTM) was 1106 in contrast to a GMT of 80 for healthy controls (p less than 0.001). No IgM anti-VCA antibody was detected, but three patients had IgA anti-VCA antibodies. Fourteen patients had elevated anti-early antigen (EA) titers (GMT = 177) which were indicative of active infections with EBV. Seven of the 14 patients demonstrated the diffuse component antibody of the early antigen (EA) complex at a GMT of 98. Anti-Epstein-Barr virus nuclear antigen (EBNA) was detected in sera of 16 patients (GMT = 64, controls GMT = 79). It is noteworthy that three patients with anti-VCA titers lacked anti-EBNA titers. These anti-body responses suggest that immunodeficiency secondary to HCL allowed reactivation of the virus.

Adult↗
PubMed1981

Spironolactone bodies in aldosteronomas and in the attached adrenals. Enzyme histochemical study of 19 cases of primary aldosteronism and a case of aldosteronism due to bilateral diffuse hyperplasia of the zona glomerulosa.

The formation of spironolactone (S) bodies, eosinophilic laminated cytoplasmic inclusions, is induced in the aldosterone-producing cells of the human adrenal cortex after the administration of spironolactone. The aim of this study was to define the enzyme histochemical characteristics of S bodies, S-body-containing cells, and the apparently hyperplastic zona glomerulosa (zG) of adrenal tissues attached to aldosteronomas. S bodies were found in 14 of 19 aldosteronomas, in 10 of 19 adrenal tissues attached to aldosteronomas, and in the adrenal tissues in a patient with aldosteronism due to bilateral diffuse zG hyperplasia. The S bodies themselves exhibited most intense 3 beta-hydroxysteroid dehydrogenase (3 beta HSD) activity but did not exhibit glucose-6-phosphate dehydrogenase (G6PD), NADP-dependent isocitrate dehydrogenase (NADP-ICDH), or succinate dehydrogenase (SDH) activity, confirming histochemically the origin of S bodies in the smooth endoplasmic reticulum. In two adenomas, S bodies were found to be surrounded by reaction products of acid hydrolase but were not found in the other adenomas and the remaining adrenal tissues. S-body-containing cells, irrespective of being neoplastic or not, showed enhanced 3 beta HSD, G6PD, and NADP-ICDH activity and weak SDH activity (Type I pattern of enzyme activity). Though zG was hyperplastic in most of the adrenal tissues attached to the adenomas, zG cells that did not contain S bodies showed the opposite pattern (Type II pattern) of enzyme activity (ie, weak 3 beta HSD, G6PD, and NADP-ICDH activity and intense SDH activity), in contrast to those in the adrenal tissues in a patient with aldosteronism due to bilateral diffuse zG hyperplasia (which showed the Type I pattern). The results are consistent with the view that hyperplastic zG cells, except S-body-containing cells, in the case of aldosteronoma are not hyperfunctioning. The latter cells may have enhanced but possibly abortive steroidogenic activity.

Adenoma↗
PubMed1980

Significance of leukocyte alkaline phosphatase in hairy cell leukemia.

Peripheral blood samples of 23 patients who had hairy cell leukemia were examined for leukocyte alkaline phosphatase scores. Leukocyte alkaline phosphatase levels were elevated in seventeen patients (74%), were normal in six (26%), and were not decreased in any patient. The mean leukocyte alkaline phosphatase score was 166 (normal range, 11-95). High leukocyte alkaline phosphatase scores correlated inversely with the absolute number of neutrophils in peripheral blood and were unrelated to other clinical factors, including age and time since splenectomy. High leukocyte alkaline phosphatase scores were also associated with a shift to more immature forms of neutrophil differentiation. These findings are consistent with the view that high leukocyte alkaline phosphatase levels in hairy cell leukemia are secondary to decreased marrow granulocyte reserves and do not necessarily indicate the existence of primary abnormalities of multiple bone marrow cell lines, including the granulocytes, in hairy cell leukemia. The role of the leukocyte alkaline phosphatase assay in the differential diagnosis of hairy cell leukemia with respect to non-Hodgkin's lymphoma, chronic lymphocytic leukemia, and other lymphoid malignancies is discussed.

Adult↗
PubMed1980

B-lineage prolymphocytic leukemia as a distinct clinicopathologic entity.

Prolymphocytic leukemia (PLL) was described originally by Galton and his colleagues as a special variant of chronic lymphocytic leukemia (CLL) with distinct clinical and hematologic features. Subsequent authors have described considerable heterogeneity in the clinical and immunologic features of PLL but have not dealt in detail with pathologic descriptions of their cases. Consequently, one might question whether PLL represents one disorder or a heterogeneous group of disorders. Three patients who satisfied Galton's original diagnostic criteria for PLL were examined by pathologic and immunologic methods. The spleens of these patients showed distinctive pseudonodular pathologic features. The malignant prolymphocytes were characterized as B-lineage by the presence of surface IgM and IgD, the expression of HLA-DR antigen, the absence of E-rosette formation, and the absence of natural killer cell activity. The existence of B-lineage prolymphocytic leukemia is affirmed as a distinct entity with unique pathologic, clinical, and immunobiologic characteristics.

Aged↗
PubMed1979

Effect of exogenous glucocorticoids given during the terminal stages on the adrenocortical histology.

The adrenal histology of 107 adult autopsy cases hospitalized within 8 days before death was examined. Thirteen (21.7%) of 60 cases given glucocorticoids within 24 hr of death showed the zona reticularis and lower zona fasciculata composed of clear type cells, while only two (4.2%) of the remaining 47 cases without corticoid administration showed the same histology. The difference was significant (p < 0.05), and it was referred to the exogenous corticoids. Administration of adrenalin and/or noradrenalin did not significantly influence the adrenocortical histology. The effect of the corticoids depended partly on the diseases from which the patients suffered: the effect was not observed in cases of severe infection or myocardial infarction, but it was shown in cases of cerebral apoplexy and miscellaneous diseases. The above results, on the basis of clear/compact cell morphology, suggest that exogenous glucocorticoids occasionally suppress the pituitary release of ACTH under the stress of dying.

Adrenal Cortex↗
PubMed1978

Enzyme histochemical and electron microscopic study of a virilizing adrenocortical adenoma.

Enzyme histochemical and ultrastructural studies of a "dexamethasone-suppressed" virilizing adrenocortical adenoma and the attached cortex revealed that tumor cells showed little activities of some lysosomal enzymes and scarcity of lipofuscins and dense bodies of lysosomal type, forming a marked contrast to the cells of zona reticularis and the virilizing adenomas previously reported. The other findings of tumor cells, such as a pattern of activities of dehydrogenases including 3beta-hydroxysteroid dehydrogenase and the morphology of mitochondria, were those of reticularis cells. The findings showed that scantiness of lipofuscins did not rule out the possibility of adenoma producing adrenal androgen, dehydroepiandrosterone. Most of the tumor cells as well as reticularis cells were positive for alkaline phosphatase, the activity of which was interpreted as the effect of ACTH stimulation.

3-Hydroxysteroid Dehydrogenases↗
PubMed1977

Correlation of alkaline phosphatase staining of cortisol-producing adrenocortical tumors with dexamethasone suppression and ACTH stimulation.

The histochemical activity of alkaline phosphatase (Al-Pase), the induction of which is one of the effects of ACTH on the adrenocortical cells, was examined in 10 adrenocortical tumors causing Cushing's syndrome and in 65 adrenal cortices. All of the compact cells in every gland, and almost all, about half, or a small proportion in the four tumors showed Al-Pase activity. These tumors decreased in steroidogenesis after the administration of dexamethasone. No compact cells exhibited the activity in six tumors, none of which was "dexamethasone-suppressible". Three of the seven attached glands examined were halfway between those of typical Cushing's syndrome and those of other than Cushing's syndrome from the viewpoint of compact/clear cell morphology. All of the tumors that had Al-Pase-positive clear cells increased in steroidogenesis after ACTH administration. These results suggested that Al-Pase activity of tumor cells was also ACTH effect and that a decrease in steroidogenesis of tumors after dexamethasone administration was due not to fluctuations but to suppression of intrinsic ACTH.

Adolescent↗