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Biomedical subjects

M Adolphe

Publications and source records attributed to M Adolphe.

At least 91 records · Page 5Linked to original sources
PubMed1982

Effect of sodium cis-beta-4-methoxybenzoyl-beta-bromacrylate (Cytembena) on HeLa cell kinetics.

The treatment of HeLa cells with various concentrations of sodium beta-4-methoxybenzoyl-beta-bromacrylate (Cytembena) results in inhibition of growth and modification of cell cycle distribution. These phenomena were observed at concentrations between 7.5 x 10(-5) and 2.5 x 10(-5) M. The estimation of DNA content by flow cytometry showed an important shift in the distribution of cycling cells with a relative decrease of G0 + G1 cells and a striking accumulation of G2 + M cells. According to our experimental conditions, the blocking up in G2 + M is irreversible at 7.5 and 5 x 10(-5) M.

Acrylates↗
PubMed1982

Effects of oxazaphosphorine cytostatics on granuloid progenitor cell (CFUc) proliferation in mice.

The effects of oxazaphosphorine cytostatics were studied on granulocytic/monocytic colony forming cells from mice bone marrow in methylcellulose culture. Cyclophosphamide, ifosfamide, trofosfamide and two secondary metabolites show a weak activity in vitro (Inhibitory Dose--50% (ID50) between 5 X 10(-4) M and 5 X 10(-5) M). By contrast, a high cytostatic activity was observed with phosphoramide mustard and especially with hydroperoxycyclophosphamide (ID50: 2.5 X 10(-6) and 4 X 10(-7) M). These results suggest that these metabolites are active. The differentiation of the three types of colonies (granulocytic, monocytic and mixed) showed no specific effect of the drugs for a given series. After in vivo treatment, cyclophosphamide, ifosfamide and trofosfamide induce an important decrease of nucleated bone marrow cells. This decrease is maximum on the third day and regresses when the treatment is interrupted. On the first day of the culture an inhibition of the proliferation of granulocytic/monocytic progenitor cells is observed. An important statistically significant stimulation of these same progenitor cells is however noted later.

Alkylating Agents↗
PubMed1981

[Comparison of the antimitotic properties of cyclophosphamide and its biotransformation products on meristema plant cells and animal cells in in vitro culture].

N-mustard diamide phosphoric and above all 4-hydroperoxycyclophosphamide are clearly more cytotoxic than same kind, which is in accordance with the fact that these compounds are the active metabolites of cyclophosphamide. On the contrary, 4-ketocyclophosphamide and carboxyphosphamide, secondary metabolites inapt to be activated, are not more cytotoxic than cyclophosphamide. No tolerance does occur with these metabolites, contrary to cyclophosphamide itself.

Animals↗
PubMed1981

Effect of three alkylating antimitotic agents and their metabolites on in vitro monogranulocytic colony forming cells from mouse bone marrow.

The effects of oxazaphosphorine cytostatics were studied on monogranulocytic colony forming cells from mouse bone marrow in methylcellulose culture. Cyclophosphamide, ifosfamide, trofosfamide and two secondary metabolites showed a weak activity (ED50 between 5 x 10(-4) mol/l and 5 x 10(-5) mol/l). On the contrary, a high toxicity was observed with phosphoramide mustard and especially hydroperoxycyclophosphamide (ED50: 2.5 x 10(-6) mol/l and 4 x 10(-7) mol/l). These results suggest that these metabolites are the carriers of cytotoxic specificity. The differentiation of the three types of colonies (granulocytic, monocytic and mixed) revealed no specific effect of the drugs for a given series. On the other hand, the staining of colonies revealed some mitotic abnormalities (agglutination bridge, micronuclei and cytodieretic alteration).

Alkylating Agents↗
PubMed1981

Fibroblast cultures in experimental disturbed metabolic conditions. effects of anti-inflammatory drugs.

Fibroblasts in normal metabolic conditions constitute a simple cellular model for the study of anti-inflammatory drugs in culture, but do not take into account the factors related to the acute inflammatory reaction. A new approach was tried in submitting the fibroblasts to the action of a mouse peritoneal exudate or selected activated macrophages. Disrupted peritoneal material was inoculated to cultures in a volume representing a number of cells corresponding to 25 or 50% of fibroblast concentration at day 0 of the experimental time. Three anti-inflammatory drugs were retained to study their effects on proliferation and metabolic parameters: hydrocortisone, indomethacin and a newly-described molecule, the methoxybenzalthiosemicarbazone (MBT). At day 0, 1, and 2, fibroblasts were counted and total cellular protein and cellular glycolytic activity were determined. 4.5 X 10(-5) M hydrocortisone and MBT protected the fibroblast from the antiproliferative action of peritoneal exudate whereas in experiments using activated macrophage homogenate, all drugs exerted a protective and stimulatory effect.

Animals↗
PubMed1980

[Inhibitory effect of cytembena (sodium-cis-beta-methoxybenzoyl-beta-bromoacrylate) on the cell kinetics of HeLa cells in culture].

The inhibiting effect of Cytembena on HeLa cell kinetics has been demonstrated and analyzed. The percentage of cycling cells decreases, according to the concentration, between 7.5 and 2.5 x 10(-5) M. Estimation of DNA by cell flow cytophotometry shows an important shift in the distribution of cycling cells with a relative decrease of G1 cells and a very important accumulation of G2 cells. According to our experimental conditions, the blocking up in G2 is irreversible only at 7.5 x 10(-5) M.

Acrylates↗
PubMed1980

Studies on in vitro colony formation by mouse bone marrow cells using different inflammatory pleural exudates. Relation to colony stimulating factor (CSF).

An inflammatory exudate obtained in Swiss mice 3 hours after intrapleural injection of dextran was able to increase the number of cultivated peritoneal macrophages in S phase. This exudate was also able to stimulate the formation of colonies from mice bone marrow progenitors of macrophages and granulocytes in methylcellulose culture. The stimulating activity of this acute inflammatory exudate was compared with that of colony stimulating factor (GM.CSF). The qualitative and quantitative results showed that the biological activity of the mitogenic factor of this inflammatory exudate, inflammatory mitogenic factor (IMF) was very close to GM.CSF at optimal concentration, but when used at the same concentration, the exudate was less active than GM.CSF. A stimulating activity was also found with another inflammatory pleural exudate induced by calcium pyrophosphate. The comparative kinetics of the action of the two exudates on CFUc appeared alike but the rise was earlier with calcium pyrophosphate. These results suggest the release of a growth factor for monocyte-macrophage in different acute inflammatory exudates.

Animals↗
PubMed1980

Effect of in vitro treatment with indomethacin on mouse granulocyte-macrophage colony-forming cells in culture (CFUC). Possible role of prostaglandins.

The effect of indomethacin (6 mg/kg daily orally for 2, 3 or 4 days) on myelopoiesis was studied in mice by estimating 1) differential cell counts of bone marrow, 2) proliferation of the CFUC in presence of GM-CSF, 3) proliferative state of CFUC after tritiated thymidine suicide. After 4 days of treatment, a rise in the recognizable myeloid precursors, an increase of both the colony forming capacity and the number of CFUC in S-phase were observed. These data suggest that indomethacin produced an hyperplasia of the committed stem cell compartment. The decrease of PGE2 amounts in bone marrow cells following treatment with indomethacin could explain the hyperplasia observed. These in vivo results are in accordance with the in vitro data which show that PG could control the proliferation and differentiation of myeloid progenitor cells.

Animals↗
PubMed1979

Effect of human chorionic gonadotrophin on phagocytic activity and proliferative capacity of rat peritoneal macrophages in culture.

Human chorionic gonadotrophic hormone (hCG) decreased phagocytosis of rat peritoneal macrophages in culture and also inhibited their capacity to be stimulated by an inflammatory exudate. Both effects were related to the concentration used. These experimental results suggest that elevated levels of hCG may play a role in the prevention of the rejection of the foetal allograft by the maternal host.

Animals↗
PubMed1978

Effect of in vivo treatment by dexamethasone and indometacin on DNA synthesis in rat macrophages in culture stimulated by inflammatory exudate.

The acute inflammatory exudate obtained 4 hr after intrapleural injection of dextran in rats is able to induce DNA synthesis and division of normal rat macrophages in culture. The influence on this phenomenon of two types of pretreatment of rats with dexamethasone or indometacin has been investigated. Exudates of rats treated with dexamethasone decreased the DNA synthesis in control macrophages. On the contrary, exudates of rats treated with indometacin had no effect on this DNA synthesis. These results could be explained by the different actions of the two anti-inflammatory agents on cellular migration in pleural exudates. In macrophages harvested from rats treated with dexamethasone or indometacin the DNA synthesis induced by inflammatory exudates was decreased. This identical response elicited by these two agents could be related to their direct antimitotic activity on macrophages.

Animals↗