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Biomedical subjects

M Adinolfi

Publications and source records attributed to M Adinolfi.

At least 145 records · Page 8Linked to original sources
PubMed1967

Observations on the levels of gamma G, gamma A, gamma M globulins, anti-A and anti-B agglutinins, and antibodies to Escherichia coli in Down's anomaly.

The levels of gammaG, gammaA, and gammaM globulins were estimated in a group of patients with Down's anomaly and in groups of mentally retarded children and normal children, matched for age and sex, using an immunological method. Higher levels of gammaG globulin were observed in patients with Down's syndrome, in association with a small but significant lower concentration of gammaM globulin. The titres of anti-A and anti-B agglutinins and antibodies to Escherichia coli, estimated before and after treatment of serum with 2-mercaptoethanol, were found to be within normal levels. These data seem to suggest that patients with Down's syndrome do not produce ;faulty' immunoglobulins as has been previously postulated. It is suggested that the abnormal levels of immunoglobulins found in Down's anomaly are not peculiar to patients with Down's syndrome but occur in other disorders in which the reticuloendothelial system or the lymphocytes are involved.

Adolescent↗
PubMed1966

Gamma-A-blood group antibodies.

The serological characteristics of gammaA-anti-A and anti-B were studied using, as a source, either colostrum, or fractions relatively rich in gammaA obtained from selected potent antisera. gammaA-anti-A and anti-B were never hemolytic nor did they sensitize red cells to agglutination by anticomplement globulin sera. gammaA-anti-A, like gammaG-anti-A and unlike gammaM-anti-A was unaffected by heating at 56 degrees C for 3 hr. On the other hand in the following three characteristics the behavior of gammaA fell between that of gammaG- or gammaM-anti-A: sensitivity to inactivation by 2-mercaptoethanol, ease of neutralization by A substance and degree of enhancement of agglutination in a medium of serum rather than saline. The agglutination produced by gammaA-anti-A was regularly enhanced by addition of anti-gammaA-globulin serum. In searching for gammaA-blood group antibodies of other specificities the following sera were tested: anti-D (32 examples); anti-c (2 examples); anti-Le(a) or -Le(b) (3 examples); anti-K (3 examples); anti-Fy(a) (3 examples), and anti-Jk(a) (3 examples). Only 3 sera, all containing anti-D, sensitized red cells to agglutination by anti-gammaA. There were no discrepancies between results obtained with four different anti-gammaA-globulin sera. Approximately half the sera were fractionated on DEAE-cellulose, and the fractions rich in gammaA tested for their ability to sensitize red cells to agglutination by anti-gammaA; no additional examples of gammaA-antibodies were detected. One of the three examples of gammaA-anti-D appeared in the serum of a woman during the course of deliberate reimmunization. gammaA-anti-D appeared only after three intravenous injections of red cells although the gammaG-anti-D titer rose considerably after a single injection. 3 yr after a fourth injection of Rh-positive cells gammaA-anti-D, as well as gammaG-anti-D, was still present in the serum.

Blood Group Antigens↗
PubMedDate not supplied

First trimester prenatal diagnosis using transcervical cells: an evaluation.

Human trophoblastic cells can be retrieved by minimally invasive procedures from the endocervical canal between between 6 and 15 weeks gestation. The incidence with which fetal cells can be detected in transcervical cell (TCC) samples varies according to the method of collection and the molecular techniques employed for their identification. Fluorescence in-situ hybridization (FISH) and polymerase chain reaction (PCR) assays have been successfully used to detect aneuploidies and Y-derived DNA sequences in TCC samples obtained from male fetuses. Chromosome specific polymorphic DNA sequences (small tandem repeats) have also been employed to identify, by quantitative fluorescent PCR, fetal cells in TCC samples. Furthermore, Rh(D) sequences have been amplified in samples retrieved from Rh(D) negative mothers. Preliminary results also suggest that prenatal diagnoses of thalassaemia and sickle cell anaemia can be performed on clumps of cells isolated from TCC samples. Overall systematic studies allow optimism about the possibility of using TCC samples for the prenatal diagnosis of selected inherited disorders.

Cervix Uteri↗